In vitro Regeneration of Ginger (Zingiber officinale Roscoe)

Abstract

An efficient and reproducible in vitro regeneration protocol was established for two varieties of ginger (Z. officinale Roscoe) namely, BARI Ada-1 and Chinese ginger accession number SG876). In case of BARI Ada-1 best result was obtained on MS supplemented with 2.0 mg/l BAP, 0.5 mg/l Kn and 0.5 mg/l NAA. In this combination, 95% rhizome bud explants responded within 6 - 8 days and mean number of shoots per explant was 8.79 ± 0.42. On the other hand, Chinese ginger showed best (90%) shoot regeneration response from the same explants on the same medium and hormonal combinations but in exchange of 0.25 mg/l NAA. In this hormonal composition shoot initiation started within 7 - 8 days of culture and mean number of shoots/explant was 6.83 ± 0.71 after 24 - 27 days of culture. Maximum root induction (90 and 80%) was found on MS supplemented with 0.5 mg/l IBA and 0.5 mg/l NAA in case of BARI Ada-1 and Chinese ginger, respectively. The in vitro regenerated plantlets were successfully transplanted into the soil after acclimatization.

In vitro propagation of red ginger (Zingiber officinale Roxb. var. Rubrum) in different concentrations of sucrose and growth regulator

“…In vitro propagation method has been used to generate a massive amount of disease-free clonal plants rapidly [2,12]. Rapid micropropagation of ginger has developed through direct organogenesis [13,14,15,16] and callus culture [10,11,17], however, in vitro plant tissue culture is highly influenced by medium formulations such as sucrose and growth regulator. The current study aimed to determine the influence of sucrose concentration and growth regulators to articulate an effective protocol for shoot proliferation, elongation, and formation of in vitro roots of red ginger.…”