In vitro regeneration of Pinus brutia Ten. var. eldarica (Medw.) through organogenesis

Bello-Bello, Jericó Jabín; Lourdes, Iglesias-Andreu; Lázaro, Sánchez-Velásquez; José, C; Santana-Buzzy, Nancy

doi:10.5897/ajb12.2180

Cited by 8 publications

(4 citation statements)

References 27 publications

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“…Plant in vitro culture has greatly contributed to study their growth and development and the influence of the environmental factors on them [4], as well as the large scale propagation of threatened species [5]. However, micropropagation of woody plant species in general has a number of problems, such as a difficult rooting of shootlets [6], shoot tip necrosis [7], phenolization [8,9], hyperhydricity [10] and a low morphogenetic capacity of the in vitro shoots [11]. In P. friedrichsthalianum, one of the main problems for in vitro propagation of is the presence of contaminants during culture initiation and the subsequent low viability of explants to proceed to the multiplication phase.…”

Section: Introductionmentioning

confidence: 99%

Argovit™ silver nanoparticles reduce contamination levels and improve morphological growth in the in vitro culture of Psidium friedrichsthalianum (O. Berg) Nied.

et al. 2020

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Psidium friedrichsthalianum (O. Berg) Nied. (Cas) is a guava species from Myrtaceae family whose fruits are very attractive for their organoleptic properties, its economic and nutritional importance and its use as rootstocks. One of the main problems for in vitro propagation of Cas is contamination, and silver nanoparticles (AgNPs), are the most useful in the treatment of plant tissues due to their microbicidal action. Thus, the microbicidal effect of Argovit™ AgNPs (suspension in water, with PVP 18.8 wt% and metallic silver 1.2 wt%, in spheroids form of 35 ± 15 nm, hydrodynamic diameter of 70 nm and zeta potential of − 15 mV) was evaluated either, through the disinfection of Cas shoot tips explants or by dispensing a liquid layer of AgNPs onto the semisolid culture medium during the in vitro establishment phase. The effect of Argovit™ silver nanoparticles in the multiplication rate and the foliar area of Cas was also evaluated. Application of AgNPs 50 mg/L directly to shoot tips during 5 min yielded a contamination rate of 40%, while culture medium sterilization with 5 mg/L of AgNPs as a permanent bilayer reduced shoot contamination rate to 50% compared to 80% for the controls. In addition, Argovit™ silver nanoparticles also enhanced foliar area by 560% (from 0.073 to 0.409 cm 2) and multiplication rate by 180% (from 1.286 to 2.286 shoots/explant). The study concluded that the best way for in vitro use of AgNPs on propagation of P. friedrichsthalianum, is as sterilization agent of the culture media because produce additional advantage of enhancing the leaf area and multiplication rate of the shoots.

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Section: Introductionmentioning

confidence: 99%

Argovit™ silver nanoparticles reduce contamination levels and improve morphological growth in the in vitro culture of Psidium friedrichsthalianum (O. Berg) Nied.

et al. 2020

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“…Subsequently clonal propagation by organogenesis was achieved in Pseudotsuga menziesii (Gupta and Durzan, 1987), Pinus spp. (Diego et al, 2008(Diego et al, , 2010Cortizo et al, 2009;Bello-Bello et al, 2012;de Oliveira et al 2012), Larix deciduas (Ewald 2007), and Sequoia sempervirens (Boulay, 1978;Arnaud et al, 1993;Ahuja, 1996Ahuja, , 2017Korban and Sul, 2007). However, somatic embryogenesis has been more successfully exploited for the clonal propagation of a large number of conifer tree species using embryonal tissues and other explants.…”

Section: Introductionmentioning

confidence: 99%

Fate of forest tree biotechnology facing climate change

Ahuja

2021

Silvae Genetica

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Woody plants have been cultured in vitro since the 1930s. After that time much progress has been made in the culture of tissues, organs, cells, and protoplasts in tree species. Tree biotechnology has been making strides in clonal propagation by organogenesis and somatic embryogenesis. These regeneration studies have paved the way for gene transfer in forest trees. Transgenics from a number of forest tree species carrying a variety of recombinant genes that code for herbicide tolerance, pest resistance, lignin modification, increased woody bio-mass, and flowering control have been produced by Agrobacterium-mediated and biolistic methods, and some of them are undergoing confined field trials. Although relatively stable transgenic clones have been produced by genetic transformation in trees using organogenesis or somatic embryogenesis, there were also unintended unstable genetic events. In order to overcome the problems of randomness of transgene integration and instability reported in Agrobacterium-mediated or biolistically transformed plants, site-specific transgene insertion strategies involving clustered regularly interspaced short palindromic repeats (CRISPR-Cas9) platform offer prospects for precise genome editing in plants. Nevertheless, it is important to monitor phenotypic and genetic stability of clonal material, not just under greenhouse conditions, but also under natural field conditions. Genetically modified poplars have been commercialized in China, and eucalypts and loblolly pine are expected to be released for commercial deployment in USA. Clonal forestry and transgenic forestry have to cope with rapid global climate changes in the future. Climate change is impacting species distributions and is a significant threat to biodiversity. Therefore, it is important to deploy Strategies that will assist the survival and evolution of forest tree species facing rapid climate change. Assisted migration (managed relocation) and biotechnological approaches offer prospects for adaptation of forest trees to climate change.

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“…Therefore, many countries have sought to cultivate and raise it for the purposes of afforestation of arid areas and combating desertification and as windbreaks and afforestation in parks, roads and public squares, and it is raised as Christmas trees [2]. The method of propagation by seeds is the most common method to propagation of conifers in general as it is the easiest and cheapest, and is used as the main means of propagation of Pinus eldarica [3].…”

Section: Introductionmentioning

confidence: 99%

Effect of stratification and dipping with different concentrations of gibberellic acid in the vegetative and root growth characteristics of Pi-nus eldarica Medw.

Basheer

Ali

2021

JKAS

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This study was conducted on the seeds of Pinus eldarica during the period from February 2020 to September 2021 to find out the effect of cold stratification at (⁰4C) for (60 days) and different concentrations of gibberellic acid (0, 200,400 mg. L-1) in the traits of the vegetative and root growing seedlings. The results showed the superiority of the stratified seeds for the characteristics (seedling height, stem diameter, number of branches, number of leaves, root length, dry weight of the vegetative growth, dry weight of the root system). The results of dipping in gibberellic acid showed a superior concentration (400 mg.L-1) for traits (seedlings height, leaves number, length of the main root, dry weight of the shoot, dry weight of the root system). The concentration of 200 mg.L-1 for the two traits (stem diametet, branches number) was superior. The results of the binary interaction of stratification and gibberellic acid showed superiority of the stratified seeds with two concentrations (400 mg.L-1 and 200 mg.L-1) for traits (seedling height, eaves number, length of main root, dry weight of shoot, dry weight of root) and (Stems diameter Branches number) respectively.

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In vitro regeneration of Pinus brutia Ten. var. eldarica (Medw.) through organogenesis

Cited by 8 publications

References 27 publications

Argovit™ silver nanoparticles reduce contamination levels and improve morphological growth in the in vitro culture of Psidium friedrichsthalianum (O. Berg) Nied.

Argovit™ silver nanoparticles reduce contamination levels and improve morphological growth in the in vitro culture of Psidium friedrichsthalianum (O. Berg) Nied.

Fate of forest tree biotechnology facing climate change

Effect of stratification and dipping with different concentrations of gibberellic acid in the vegetative and root growth characteristics of Pi-nus eldarica Medw.

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