In vitro regeneration of mature Pinus sylvestris buds stored at freezing temperatures

Andersone, Una; Ievinsh, Gederts

doi:10.1007/s10535-005-1284-y

Cited by 7 publications

(2 citation statements)

References 12 publications

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“…Moreover, many biochemical changes occur during culture of mature tissues resulting in rapid tissue browning followed by deterioration of cellular ultrastructure and necrosis. Also, high percentages of infections usually occur during culture of mature tissues (Andersone and Ievinsh 2005). Plant regeneration from axillary or quiscent meristems is a widely used method as it results in formation of genetically identical individuals.…”

Section: Discussionmentioning

confidence: 99%

Plantlet regeneration from fascicular buds of seedling shoot apices of Pinus roxburghii Sarg

Kalia¹,

Arya²,

Kalia³

et al. 2007

Biologia plant.

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Shoot apices of Pinus roxburghii Sarg were cultured on Murashige and Skoog's medium (MS) supplemented with cytokinins 6-benzyladenine (BA), kinetin and N-benzyl-9-(2-tetrahydropyranyl) adenine (BPA) alone and in combination with auxin, -napthaleneacetic acid (NAA). Of the three cytokinins tested at varying concentrations, medium supplemented with 10 μM BA was found optimal in respect of explant responsiveness (97.22 %) and average number of buds induced per explant (7.42). The concentration of cytokinins in the induction medium had a profound effect on rate of elongation of induced buds on MS basal medium containing 0.5 % activated charcoal. Further, shoots induced on lower concentrations of BA increased up to 2.4 times in length in 4 weeks. Decapitation of the explant enhanced the rate of axillary bud elongation. Proliferating shoot cultures were established by sub-culturing the axillary shoots on MS supplemented with 10 μM BA. Shoots 2 -3 cm in length were suitable for culturing as more buds were induced on them compared to longer or shorter shoots. Root primordia were induced on 70.83 % shoots when transferred to ½ MS medium supplemented with 5.0 μM NAA. Elongation of root primordia (60 %) was achieved in liquid ½ MS basal medium. The plantlets were successfully transferred to soil after hardening; the time period from initiation of shoot buds to transplantation being 20 -22 weeks.

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Section: Discussionmentioning

confidence: 99%

Plantlet regeneration from fascicular buds of seedling shoot apices of Pinus roxburghii Sarg

Kalia¹,

Arya²,

Kalia³

et al. 2007

Biologia plant.

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“…The onset of these developmental changes appears to make the tissues temporarily more responsive to in vitro stimuli (Bonga 1997(Bonga , 2004. This process was stimulated by 3-month storage at freezing temperatures, which presumably acts as a mild stress (Bonga 1997;Andersone and Ievinsh 2005). An unusual feature of the L. decidua somatic embryos was that initially they were incapable of switching from the embryogenic to the vegetative mode, with new adventitious embryos forming from various parts of the primary and secondary ones before finally developing proper shoots (Bonga 1996).…”

Section: Position and Timingmentioning

confidence: 99%

Recalcitrance in clonal propagation, in particular of conifers

Bonga

Klimaszewska

Aderkas

2009

Plant Cell Tiss Organ Cult

173

136

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Despite major advances in forest biotechnology, clonal regeneration by somatic embryogenesis or organogenesis is still difficult for many woody species and is often limited to the use of juvenile explants. Adventitious regeneration of plants from gymnosperms older than zygotic embryos, and frequently even from highly immature zygotic embryos, is often difficult or has not yet been achieved. A number of experimental approaches that could eventually lead to overcoming recalcitrance are suggested in this review. When cloning trees of various ages, it is important to determine first which part of the individual contains the most responsive cells and at what time of the year these cells are in the most responsive state. This allows selection of the most useful explants. In hardwood trees and a few gymnosperms, responsive tissues are found in root or stump sprouts and in tissues near the site of meiosis at about the time that meiosis takes place. Another potentially active area is the shoot apex with most or all of its leaf or needle primordia removed. Apomixis is a natural form of clonal regeneration but occurs naturally in only one gymnosperm species. As the genetic mechanism of apomixis has been in part elucidated, the induction of apomixis by experimental means may soon be possible. The cytoplasm plays a major role in the expression or repression of nuclear genes that control embryogenesis. Expression of nuclear genes can be manipulated by nuclear transfer into de-nucleated cells (e.g., the cytoplasm of egg cells). Cytoplasmic control also plays a role in regeneration by androgenesis, asymmetric cell division and cell isolation. A short overview is presented of the genetic mechanisms involved in embryo initiation, maturation and germination and how manipulation of these mechanisms by genetic transformation could help in overcoming recalcitrance. It is expected that rapid development in the fields of research areas discussed in this review will over time eliminate the problem of recalcitrance in many instances where it is currently prevalent.

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In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir.

2006

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An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and 5.2 shoots per leaf explant were achieved when cultured on a medium containing 10 microM 6-benzylaminopurine (BAP) and 2.5 microM 1-naphthaleneacetic acid (NAA). Shoot organogenesis was improved further when the leaf explants were pre-incubated at low temperature, and 80.6% of shoot regeneration frequency was recorded with 9.3 shoots per leaf explant at 4 degrees C by 5-day pretreatment period. Up to 87.0% of the regenerated shoots formed complete plantlets on a medium containing 2.5 microM indole-3-acetic acid (IAA) within 28 days, and 85.2% of the regenerated plantlets survived and grew vigorously in greenhouse condition. The phytochemical profile of the micropropagated plants was similar to that of wild plants. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of the elite medicinal plant.

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In vitro regeneration of mature Pinus sylvestris buds stored at freezing temperatures

Cited by 7 publications

References 12 publications

Plantlet regeneration from fascicular buds of seedling shoot apices of Pinus roxburghii Sarg

Plantlet regeneration from fascicular buds of seedling shoot apices of Pinus roxburghii Sarg

Recalcitrance in clonal propagation, in particular of conifers

In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir.

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