In vitro regeneration of Allophylus serratus. Roxb (Kurz), an important medicinal plant

Abstract

Background Allophylus serratus Roxb (Kurz.) is a medicinal plant with various therapeutic properties. It has been used for the treatment of various alignments such as elephantiasis, edema, fractured bones, in bone dislocations, wound, several gastrointestinal disorders such as dyspepsia, anorexia, and diarrhea. The aim of this study is to establish protocol for surface sterilization, in vitro callus induction and plant regeneration from leaf and nodal segment explants of Allophylus serratus. Results From the tested media, the maximum callus induction frequency (81.11%) from leaf and 84.44% from nodal segment explants were achieved on MS medium supplemented with 3 mg l− 1BAP) combined with 0.5mgl− 1 NAA. The highest shoot regeneration frequency (57.78%) and root regeneration frequency (65.55%) were achieved on growth regulators free MS medium and MS medium supplemented with 1.5 mg l− 1IBA respectively. Acclimatization of the regenerated plantlets was established successfully (100%) in a pot containing sand, soil and compost in 1:2:2 ratios. Conclusions In this study successful callus induction protocol from leaf and nodal explants of A. serratus using MS media supplemented with different concentrations and combinations of plant growth regulators (2,4-D, NAA, and BAP). MS media containing 3mg/l BAP and 0.5mg/l NAA was the most efficient for callus induction. these callus induction and plant regeneration protocols are very important for mass propagation of the species and also opens a new way to facilitate secondary metabolites production and isolation of pharmaceuticals from callus rather than harvesting the plant.