In vitro regeneration and improving kaempferol accumulation in blackberry (Rubus fruticosus L.) callus and suspension cultures

Nower, Ahmed A.; Elaziem, Tamer M. Abd

doi:10.21608/ejchem.2022.118717.5340

Cited by 2 publications

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“…The presence of TDZ and BAP in the culture medium is thought to influence A. cuprea high organogenesis potential. The combination of TDZ and BA promotes cell division, callus formation, and growth [26]. TDZ acts as a cytokinin oxidase inhibitor, preventing the degradation of adenine-type cytokinins and translocating BA to injured explant tissue parts.…”

Section: Discussionmentioning

confidence: 99%

Applicable Rapid Propagation Protocol Through Organogenesis of Alocasia Cuprea for Plant Development Using Micro-Bulblet Explant

Rachmawati,

Fibrianty,

Rianawati

et al. 2023

BIO Web Conf.

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Alocasia of Indonesian endemic has significant market potential. However, the scarcity of qualified seedlings hampered their commercialization. The purpose of this study was to obtain the best sterilization technique and media for in vitro propagation of Alocasia cuprea through the organogenesis pathway using microbulblet explants. Randomized complete block designs with five to ten replicates were applied in the experiments. Application of 5 and 10% clorox for 5 and 10 minutes resulted in 40.6% aseptic explants, 10% browning explants, and 90% explant growth potential. The best shoot initiation was achieved on the MS added 4.54 μM thidiazuron and 2.22 μM BAP. This medium had an 85% explant initiation, 4.2 shoots per explant, and 6.5 days initiation time. The best shoot proliferation was discovered on MS medium with 4.44 μM BAP, which produced 10.5 initial shoots with a shoot height of 1.3 cm and a clump diameter of 1.3 cm. The best plantlet regeneration was determined on ½ MS medium with 6.5 cm plantlet height, 3.5 leaves total, 3.8 cm leaf length, 2.6 cm leaf width, and 3.8 roots. This propagation method will be applicable to the propagation of qualified seedlings for commercial purposes and potentially used for other endemic Alocasia.

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Section: Discussionmentioning

confidence: 99%

Applicable Rapid Propagation Protocol Through Organogenesis of Alocasia Cuprea for Plant Development Using Micro-Bulblet Explant

Rachmawati,

Fibrianty,

Rianawati

et al. 2023

BIO Web Conf.

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“…3% sucrose and 6 to 7 g/l were added to the agar. MS medium and auxin IBA or NAA (between 0.01 and 0.5 mg/l) and cytokinin BAP (between 0.5 and 1.0 mg/l) showed the best results [37,47] The combination of BA and TDZ used in culturing blackberry explants had a great impact on the amount and frequency of shoot induction with a high number of shoot lengths from the initial stage and the highest adventitious bud formation [48].…”

Section: Sterilization Methods and Initiation Stepmentioning

confidence: 99%

Methods of Blackberry Propagation in vitro Condition

Dönmez,

Polat,

Hamakhan

et al. 2024

BIO Web Conf.

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Blackberry, one of the most important fruit species belonging to the Rosaceae family, can be grown in different environments due to having wide adaptation ability. Although it originated in Europe, today most of the common cultivars have North American origin. Also, expansion of its production last 25 years especially in Europe and the USA, the blackberry has become the fourth berry in the fresh berry market after strawberries, blueberries, and raspberries. Blackberry fruits are rich in vitamins, polyphenols, minerals, and antioxidants, especially gallic acid and routine. Many studies have proven that high nutritional composition has a positive effect on human health in preventing various diseases. It has an important place in the fresh and processed market. Frozen fruits can be used processed such as an ice cream, juice, jam, marmalade, cake, and sweet products. Blackberry breeding studies have been ongoing for over 100 years to increase yield and fruit quality, thornless cane, to improve disease insect resistance, and cane management and primocane fruiting. In vitro propagation is an alternative method to introduce new cultivars quickly into the market and to provide disease-free planting material compared with traditional methods. The purpose of this study was to summarize blackberry propagation methods in vitro conditions.

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In vitro regeneration and improving kaempferol accumulation in blackberry (Rubus fruticosus L.) callus and suspension cultures

Cited by 2 publications

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Applicable Rapid Propagation Protocol Through Organogenesis of Alocasia Cuprea for Plant Development Using Micro-Bulblet Explant

Applicable Rapid Propagation Protocol Through Organogenesis of Alocasia Cuprea for Plant Development Using Micro-Bulblet Explant

Methods of Blackberry Propagation in vitro Condition

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