In vitro regeneration and genetic transformation of common bean (Phaseolus vulgaris L.)—Problems and progress

Veltcheva, M.; Svetleva, D.; Petkova, S.; Perl, Avihai

doi:10.1016/j.scienta.2005.07.005

Cited by 47 publications

(33 citation statements)

References 70 publications

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“…Grain legumes are generally considered to be recalcitrant to in vitro regeneration and transformation (Veltcheva et al, 2005;HnatuszkoKonka et al, 2014); though some successes have been reported. Through in vitro regeneration of pigeon pea, somaclonal variants that are resistant to Fusarium wilt and H. armigera can be obtained (Chintapalli et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

<i>In vitro </i>Regeneration of pigeon pea using leaf explants

Asande¹,

Indieka²,

Adero³

et al. 2016

Afr. Crop Sci. J.

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Section: Introductionmentioning

confidence: 99%

<i>In vitro </i>Regeneration of pigeon pea using leaf explants

Asande¹,

Indieka²,

Adero³

et al. 2016

Afr. Crop Sci. J.

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“…As a consequence, novel traits from other species cannot be inserted into common bean. Therefore, genetic transformations are needed to allow breeders to introduce novel traits that could contribute to improved performance and quality, tolerance to abiotic and biotic factors that limit yield and reduce profitability (Aragao et al , 1998(Aragao et al , 2001Veltcheva et al 2005).…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration and morphogenesis studies in common bean

Kwapata

Sabzikar

Sticklen

et al. 2009

Plant Cell Tiss Organ Cult

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An efficient protocol for high frequency in vitro regeneration of multiple shoots and somatic embryos from the embryonic axis of common bean (Phaseolus vulgaris) was developed. Ten common bean cultivars representing a wide range of diversity among current commercial market classes were used for in vitro regeneration evaluation in our study. These cultivars were tested on 63 different media formulations consisting of combinations of cytokinins, namely benzyladenine (BA) and thidiazuron (TDZ) at concentration levels of 0.0, 1.0, 2.5, 5.0 and 10.0 mg l -1 and auxin, namely naphthalene acetic acid (NAA) and indole-3-acetic acid (IAA) at concentration levels of 0.0, 0.05, 0.1 and 1.0 mg l -1 . P. vulgaris cv. Olathe pinto bean performed the best producing over 20 multiple shoots per explant while cv. Condor black bean was the poorest with nine multiple shoots per explant. The optimum media for regeneration of multiple shoots was 4.4 mg l -1 Murashige and Skoog (MS) containing 2.5 mg l -1 BA and 0.1 mg l -1 IAA supplemented with 30 mg l -1 silver nitrate. Adventitious shoots and somatic embryos were regenerated on 4.4 mg l -1 MS medium containing 1 mg l -1 TDZ and 0.05 mg l -1 NAA supplemented with 30 mg l -1 silver nitrate or activated charcoal. Efficient and effective rooting of plantlets was achieved by dipping the cut end base of in vitro regenerated shoots in 1.0 mg l -1 indole-3-butyric acid (IBA) solution and culturing on media containing 4.4 mg l -1 MS supplemented by 0.1 mg l -1 IAA, NAA or IBA.

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“…Among the most important applications are studies related to plantherbivores interactions (Maffei et al 2006(Maffei et al , 2007. To date, Phaseolus species have been regenerated and transformed genetically with limited success (Christou 1997), with most of the efforts being concentrated on P. vulgaris (Franklin et al 1991;Mohamed et al 1993;Cardenas-Avila et al 2000;Svetleva et al 2003;Veltcheva et al 2005;DelgadoSánchez et al 2006), P. acutifolius (Kumar et al 1988;Malik and Saxena 1992;Mohamed et al 1992;Dillen et al 1996;De Clercq et al 2002), P. aureus (Malik and Saxena 1992), P. coccineus (Santalla et al 1998) and P. polyanthus (Zambre et al 2001).…”

mentioning

confidence: 99%

“…regeneration protocols via organogenesis using different explants have been discussed (Svetleva et al 2003;Veltcheva et al 2005;Delgado-Sánchez et al 2006), while the importance of developing legume tissue culture and their applications have been reported (Barros et al 1997;Christou 1997;Somers et al 2003) To our knowledge, no successful method has been reported for the callus induction and shoot regeneration of P. lunatus. The advantages of an efficient method for Lima bean plant regeneration are many.…”

mentioning

confidence: 99%

Callus induction and shoot regeneration of Phaseolus lunatus L. cv. Wonder Bush and cv. Pole Sieva

Kanchiswamy

Maffei

2007

Plant Cell Tiss Organ Cult

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We report the first protocol for callus induction and shoot regeneration for Phaseolus lunatus L. cv. Wonder Bush and cv. Pole Sieva. We used different explants viz., epicotyls, cotyledons and hypocotyls. The medium used was MS basal medium with thidiazuron (0.5 mg l -1 ) and IAA (0.05 mg l -1 ) for the induction of callus followed by BAP (1.0 mg l -1 ) for the induction of shoots. Epicotyl explants showed the fastest response and the highest percentage of shoot regeneration. This protocol opens new biotechnological strategies to transfer economically important genes to this important crop species.

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In vitro regeneration and genetic transformation of common bean (Phaseolus vulgaris L.)—Problems and progress

Cited by 47 publications

References 70 publications

<i>In vitro </i>Regeneration of pigeon pea using leaf explants

<i>In vitro </i>Regeneration of pigeon pea using leaf explants

In vitro regeneration and morphogenesis studies in common bean

Callus induction and shoot regeneration of Phaseolus lunatus L. cv. Wonder Bush and cv. Pole Sieva

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