In vitro Regeneration and Flower Induction on Solanum nigrum L. from Pachamalai hills of Eastern Ghats

Basha, A Kolar; Vivekanandan, Lalitha; Basha, M. Ghouse

doi:10.3329/ptcb.v18i1.3264

Cited by 7 publications

(3 citation statements)

References 3 publications

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“…Leaf explants showed high percentage of callus formation than nodal stem and shoot apex respectively (Figure 1 d-f). Similar successful induction of callus from S. nigrum L. on MS basal medium supplemented with IAA and BAP were also observed [11] . Ray et al (2011) observed the highest callusing on medium MS + 2.0 mg/l BAP and 0.5 mg/l NAA during in vitro cultivation and regeneration of S. melongena [12] .…”

Section: Resultssupporting

confidence: 74%

“…Similarly, Taghipour et al (2015) observed in case of eggplant that after three weeks some differentiated shoots were rooted and some others were needed to be subcultured on fresh rooting medium for additional root development [17] . The best rooting was obtained on MS medium containing 0.5 mg/l IBA in S. Nigrum culture [11] . In in vitro study of S. melongena, MS medium with 3.0 mg/l IBA gave the best result for root induction for average number and mean length of roots [18] .…”

Section: Resultsmentioning

confidence: 99%

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Effect of explant and phytohormone on in vitro regeneration of Solanum indicum, an important medicinal weed

Rani¹,

Deepti²,

Anjani³

et al. 2021

J Pharmacogn Phytochem

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The aim of this study was to observe the effect of different explants viz. apical shoot, nodal stem and leaf of Solanum indicum and the concentration of phytohormones on tissue culture responses during the in vitro regeneration and development of micropropagation protocol. For primary establishment of explants, 0.1% HgCl2 solution for 5 minutes was found to be most effective for surface sterilization and Murashige and Skoog (MS) medium having 3 mg/l kinetin and 3 mg/l 6-benzylaminopurine was found to be the best for all the explants. Among all three explants, leaf explants showed the best primary establishment. The explants showed callus formation after 10 to 12 days of culture. The frequency of callus formation was higher on MS medium supplemented with 3 mg/l 3-indoleacetic acid and 5 mg/l 6-benzylaminopurine by the leaf explant. The highest frequency of shooting was recorded on MS medium with 3 mg/l kinetin and 2mg/l 6-benzylaminopurine. Apical shoot showed higher potential of shooting than nodal stem and leaf explants. The in vitro regenerated elongated shoots were then excised from shoot clumps and transferred to rooting medium containing Indole butyric acid at 1.5 mg/l. The in vitro regenerated plantlets were shifted to the polycups having mixture of sterilized soil and farm yard manure in 1:1 ratio. Initially, these plants were kept under high humidity and progressively acclimatized to reduced humidity for their hardening and acclimatization. Plantlets, thus developed, were successfully established and finally transferred to a greenhouse.

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Section: Resultssupporting

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Effect of explant and phytohormone on in vitro regeneration of Solanum indicum, an important medicinal weed

Rani¹,

Deepti²,

Anjani³

et al. 2021

J Pharmacogn Phytochem

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“…In medicinal plant species like Gentiana lutea, (Petrova et al, 2011) 1 mg L -1 IBA was found effective for rooting. The best rooting was obtained on MS medium containing 0.5 mg/l IBA in Solanum nigrum (Kolar et al, 2008). On 3 mg L -1 IBA, rooting was obtained in Chlorophytum borivilianum (Bathoju and Giri, 2012).…”

Section: Discussionmentioning

confidence: 97%

Micropropagation of Litsea glutinosa (Lour) C.B

Shah¹

2013

Int. J. Biotechnol. Mol. Biol. Res.

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Litsea glutinosa (Lour) C.B (Hindi: Maida lakri) is a medicinal plant of immense pharmaceutical value. The species is critically endangered due to its indiscriminate collection as raw material for pharmaceutical industry, where it is used for manufacturing drugs for pain, arousing sexual power and in treatment of diarrhea and dysentery etc. An attempt has been made for development of in vitro propagation procedure for the species, involving four steps, namely: culture establishment, shoot multiplication, rooting and hardening. Aseptic cultures were established on Murashige and Skoog (MS) medium supplemented 10.0 µM N 6-benzyladenine (BA) using nodal segments (1 cm). Four sets of simple randomized experiment were carried out on MS medium to study the effect of four doses of each BA, GA 3 , IAA, (0, 2.5, 5.0 and 10 µM) and ascorbic acid (0, 284, 852 and 1136 µM) for in vitro shoot multiplication. MS medium supplemented with 5.0 µM BA with 852 µM ascorbic acid significantly proved optimum for in vitro shoot multiplication and resulted in 1.05 shoot number explant-1 , 1.72 node number shoot-1 and 1.79 node number explant-1 at one month after inoculation. The in vitro multiplied shoots were tested for in vitro root induction on MS culture media containing auxin IBA (Indole-3-butaric acid) treatments (0, 2.5, 5.0 and10.0 µM) in simple randomized designs experiment. MS media supplemented with 10.0 µM IBA, screened out to be significantly excellent for induction and growth of adventitious roots, resulting in 72.2% rooting and 0.72 root number explant-1 at 30 days after inoculation. The in vitro propagated plants exhibited excellent growth. Therefore, the present study recommends a four step micropropagation procedure for in vitro production of L. glutinosa plants on a commercial scale to meet the requirement of pharmaceutical industries and save the species from extinction.

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Improvement of Green Leafy Vegetables: The Role of Plant Tissue Culture and Biotechnology

Kumar

Aruna

Giridhar

2016

Plant Tissue Culture: Propagation, Conservation and Crop Improvement

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In vitro Regeneration and Flower Induction on Solanum nigrum L. from Pachamalai hills of Eastern Ghats

Cited by 7 publications

References 3 publications

Effect of explant and phytohormone on in vitro regeneration of Solanum indicum, an important medicinal weed

Effect of explant and phytohormone on in vitro regeneration of Solanum indicum, an important medicinal weed

Micropropagation of Litsea glutinosa (Lour) C.B

Improvement of Green Leafy Vegetables: The Role of Plant Tissue Culture and Biotechnology

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