In vitro reactions of human osteoblasts in culture with zirconia and alumina ceramics

Abstract

The biocompatibility of two implantable materials, zirconia and alumina ceramics, was investigated in vitro using human osteoblast cell cultures. The viability of osteoblast cells with the materials was evaluated by the methylthiazole sulfate test that revealed an absence of any cytostatic or cytotoxic effect. Cell proliferation kinetic and total protein synthesis in osteoblasts with zirconia or alumina were similar to that observed in control cells cultured on glass coverslips. Light and scanning electron microscopic examinations showed an intimate contact between osteoblasts and the substrates; well-spread cells were observed on the surfaces of both materials. Adhesion ability and morphological characteristics were preserved in osteoblast cultures with these substrates. Moreover, immunohistochemical staining in osteoblasts with zirconia and alumina showed the capacity of these cells to elaborate the extracellular matrix composed of types I and V collagen, osteocalcin, osteonectin, bone sialoprotein, and cellular fibronectin. Finally, DNA image cytometry and interphase silver-nucleolar organizer regions quantification were applied as complementary biocompatibility tests to detect any changes in DNA synthesis and cell proliferation, respectively. The results showed that neither material altered cell ploidy or cell growth rate in accordance with the absence of any inducing effect on DNA synthesis or proliferation.

Biocompatibility study of lithium disilicate and zirconium oxide ceramics for esthetic dental abutments

“…Zirconia ceramics have been reported not to have potential toxic or genotoxic effects [171819] and to present satisfactory soft tissue responses [20]. Esthetic implant abutments must minimize or reduce plaque adhesion and possess light dynamic qualities comparable to natural teeth.…”

Cell attachment and proliferation of bone marrow-derived osteoblast on zirconia of various surface treatment

“…The biomaterial-related properties of zirconia are as advantageous as those of titanium 6. Tissue reaction and stability of zirconia, which are important factors in maintaining zirconia restorations free of periodontal problems, proved to be satisfactory 7,8. Bacterial coverage and accumulation on zirconia was reported to be lower than on titanium 9,10.…”

Differentiation potential of osteoblast from cultured C2C12 cells on zirconia disk

“…It has also been reported that osteoblast cell proliferation and protein synthesis on ZrO 2 disks are similar to those on glass coverslips 40) . C2C12 cells are known as express markers for the initial differentiation to osteoblasts (ALP activity or osteocalcin) under the existence of BMP2 and ATRA.…”