Differentiation potential of osteoblast from cultured C2C12 cells on zirconia disk

Abstract

We examined the adaptability of zirconia as a fixture for implants. A mouse myoblast cell line (C2C12) was seeded on Ce-TZP and titanium disks, and on poly-L-lysine-coated glass slides. Proliferation potency was determined by cell counting and mineral induction by BMP2 was studied. The osteoblastic differentiation potency was determined by alkaline phosphatase (ALP) and alizarin red (ARS) staining. ALP activity and calcium concentrations were colorimetrically measured. The number of cells on all materials was approximately equal. ALP and ARS staining showed densely-stained images, demonstrating the induction of C2C12 cells to express the osteoblastic phenotype. RT-PCR showed that mRNA expressions of type I collagen, osteocalcin, osterix and ALP were up-regulated. With regard to ALP activity and calcium concentration of C2C12 cells, no significant differences were observed between Ce-TZP and titanium disks. We conclude that Ce-TZP has the biological activity comparable to titanium and has the utility as fixture of dental implants.

Human cellular CYBA UTR sequences increase mRNA translation without affecting the half-life of recombinant RNA transcripts

“…C2C12 cells are derived from thigh muscle of C3H mice and are frequently used for differentiation into myoblast and osteoblast cell lineages28293031. Hence this cell line was selected for screening purposes of recombinant hBMP2 mRNAs furnished with all combinations of CYBA UTR.…”

Dissolution, bioactivity and osteogenic properties of composites based on polymer and silicate or borosilicate bioactive glass

“…However, the marker needed quantifying to confirm the effect of the boron included in the glass formulation compared to the silicate glass. C2C12 cells are often used to study osteodifferentiation in presence of BMPs [42,45]. Here, their capacity to differentiate into osteoblastic cells is exploited in the presence of BAG showing promising results for osteodifferentiation.…”

Transdifferentiation of myoblasts into osteoblasts – possible use for bone therapy

“…Transdifferentiation is an ever‐expanding field with a growing number of cells found to be capable of such a process. If successful, this process may be utilised in procedures such as dental implants, a process that requires a solid bony foundation for an implant to be held firmly in place . It may also be utilised in fracture healing and in bone destruction due to tumours where bone grafts is not an ideal treatment option .…”

“…Inducers such as osteoactivin (OA) and Runx2, and inhibitors such as Paired‐like homeodomain transcription factor 2 (Pitx2) influence the outcome of the differentiation process, potentially paving the way for future bone treatments. If successful, this potential treatment may be utilised for dental implants, fracture healing, osteoporosis and bone repair postdestruction by bone tumours …”