In Vitro Protective Efficacy of Clostridium butyricum Against Fish Pathogen Infections

Gao, Qiang; Xiao, Yingping; Sun, Peng; Peng, Shi-Qing; Yin, Fei; Ma, Xiaobo; Shi, Zhaohong

doi:10.1007/s12088-013-0394-z

Cited by 31 publications

(26 citation statements)

References 21 publications

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“…CB can also produce prebiotics, including bacteriocin, lipoteichoic acid, and hydrogen, all of which serve to regulate animal antioxidation and antibacterial functions ( Pan, 2006 ; Gao et al, 2011 ; Junghare et al, 2012 ). It was reported that CB and its lipoteichoic acid components could inhibit the growth of pathogens, including E. coli , Salmonella enteritidis , and Vibrio parahaemolyticus ( Gao et al, 2011 , 2013 ). In vertebrates, CB could improve the growth, antioxidation, and immune function of broilers ( Liao et al, 2015a , b ; Zhang et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Changes in the Intestine Microbial, Digestive, and Immune-Related Genes of Litopenaeus vannamei in Response to Dietary Probiotic Clostridium butyricum Supplementation

et al. 2018

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The intestine barrier serves as the front-line defense in shrimp. Clostridium butyricum (CB) can produce butyric acid that provides energy for the intestine epithelial cells of the host. However, the effects of dietary CB on the intestine microbiome and the digestion and immunity of the host is not clear. In this study, we therefore investigated the composition and metabolic activity of the intestine microbiome, and digestive and immune-related gene expression in Litopenaeus vannamei fed with diets containing different levels of CB: basal diet (control), 2.5 × 109 CFU kg−1 diet (CB1), 5.0 × 109 CFU kg−1 diet (CB2), and 1.0 × 1010 CFU kg−1 diet (CB3) for 56 days. Dietary CB altered the composition of the intestine microbiome. Specifically, the dominant bacterial phylum Proteobacteria was enriched in the CB3 group and weakened in the CB1 and CB2 groups. The Bacteroidetes was enriched in the CB1 and CB2 groups and weakened in the CB3 group. The Firmicutes was enriched in all three CB groups. At the genus level, the potential pathogen (Desulfovibrio and Desulfobulbus) were weakened, and beneficial bacteria (Bacillus, Clostridium, Lachmoclostridium, Lachnospiraceae, and Lactobacillus) were enriched in response to dietary CB; these might contribute to the expression of the host digestive genes (α-amylase, lipase, trypsin, fatty acid-binding protein, and fatty acid synthase) and immune-related genes (prophenoloxidase, lipopolysaccharide and β-1,3-glucan binding protein, lysozyme, crustin, and superoxide dismutase). Additionally, CB enhanced the bacterial metabolism, especially that of carbohydrates, polymers, amino acids, carboxylic acids, and amines. These results revealed that dietary CB had a beneficial effect on the intestine health of L. vannamei by modulating the composition of the intestine microbiome, enhancing the microbial metabolism activity, and promoting the digestion and immunity of the host. The optimal dietary supplementation dosage was found to be 5.0 × 109 CFU kg−1 in the diet.

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Section: Introductionmentioning

confidence: 99%

Changes in the Intestine Microbial, Digestive, and Immune-Related Genes of Litopenaeus vannamei in Response to Dietary Probiotic Clostridium butyricum Supplementation

et al. 2018

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“…Clostridium butyricum found in soil and intestines of healthy animals and humans is a butyricum acid‐producing, spore‐forming, Gram‐positive and obligate anaerobic rod bacterium, which can produce endospores and be able to survive in media of low pH and relatively high bile concentration (Zhang et al., ). Moreover, C. butyricum can prevent disturbances of intestinal microflora (Gao et al., ; Song, Wu, Cai, Zhang & Zheng, ), accrete with available intestinal bacteria and modulate intestinal microbial balance, which is supposed to be a potential probiotic in animal feed (Pan, Wu, Zhang et al., ; Yang et al., ). C. butyricum has been mainly studied in human (Ito et al., ; Seki et al., ) and terrestrial animals (Kong, He, Jia, Zhu & Ruan, ; Liao et al., ; Yang et al., ; Zhang et al., ; Zhuang, Jiang, Jia & Yan, ), but rarely in aquatic organisms (Pan, Wu, Song, Tang & Zhao, ; Song et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…butyricum can prevent disturbances of intestinal microflora (Gao et al, 2013;Song, Wu, Cai, Zhang & Zheng, 2006), accrete with available intestinal bacteria and modulate intestinal microbial balance, which is supposed to be a potential probiotic in animal feed (Pan, Wu, Zhang et al, 2008;Yang et al, 2012). C. butyricum has been mainly studied in human (Ito et al, 1997;Seki et al, 2003) and terrestrial animals (Kong, He, Jia, Zhu & Ruan, 2011;Liao et al, 2015;Yang et al, 2012;Zhuang, Jiang, Jia & Yan, 2015), but rarely in aquatic organisms Song et al, 2006).…”

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confidence: 99%

Effects of dietary supplementation of probiotics on the growth, activities of digestive and non-specific immune enzymes in hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀)

Feng

Tian

et al. 2017

Aquac Res

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Effects of Bacillus cereus BC‐01, Lactobacillus acidophilus LAG01, Clostridium butyricum CBG01 and their combinations as supplementation on the growth performance, digestive enzyme activities and serum non‐specific immunity of hybrid grouper (Epinephelus lanceolatus ♂×E. fuscoguttatus ♀) were assessed. Seven different diets, that is one control diet (basal feed without any probiotics, CT) and six treatment diets containing single B. cereus (Bs), L. acidophilus (Ls) and C. butyricum (Cs) at 1.0 × 109 cfu/kg feed, and also their combinations in equal proportion at 1.0, 2.0 and 3.0 × 109 cfu/kg feed (BLC1, BLC2 and BLC3) were prepared respectively. After 60‐day feeding trial, the final weight, specific growth rate,food consumption, food conversion efficiency and apparent digestibility coefficient of fish in Ls and BLC3 were significantly higher compared with the control (p < .05). The activities of pepsin and trypsin in the intestine of fish for Ls and BLC3 were significantly higher relative to the control (p < .05). Relative to controls, significantly enhanced amylase and lipase activities in proximal intestine except for Cs and BLC1 and lipase activities in distal intestine except for Cs were observed (p < .05). Meanwhile, activities of superoxide dismutase in the serum of fish for all treatments, lysozyme and catalase in Ls and BLC3, and glutathione peroxidase except for Cs were significantly enhanced (p < .05). Based on the above, dietary supplementation of single L. acidophilus at 1.0 × 109 cfu/kg or combination of three strains at 3.0 × 109 cfu/kg for hybrid grouper is recommended.

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“…2008) and the production of organic acids during the process of fermentation (Gao et al . 2013). In addition, C. butyricum can stimulate the activities of antioxidant enzymes (serum superoxide dismutase (SOD), phenoloxidase, acid phosphatases and lysozyme) and level of immunoglobulin M (IgM) (Song et al .…”

Section: Mechanisms Of Actionmentioning

confidence: 99%

Clostridium butyricum: a promising probiotic confers positive health benefits in aquatic animals

Tran

et al. 2020

Reviews in Aquaculture

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Clostridium butyricum is a bacterium of rod shape, strictly anaerobic, Gram‐positive and form spores. Short‐chain fatty acids, biofuel compounds and precursors of biomaterials (H2, butanol and 1,3‐propanediol) are produced by C. butyricum during the fermentation of carbohydrates and sugars. In humans and animals, although C. butyricum has been found to be associated with botulism in infant and necrotising enterocolitis in preterm neonates, some strains have been demonstrated to be beneficial as probiotics. In aquatic animals, the application of C. butyricum as a probiotic has been commonly considered. In this review, we described and documented what we knew about the isolation, identification, phenotypic and biochemical characteristics, safety, pathogenicity and the mechanisms of action of C. butyricum in the gastrointestinal tract of aquatic animals. The evidence on the roles that C. butyricum plays in improving growth performance, digestibility, survival rate, immune response, disease resistance and the structure of intestinal tract, as well as the modulation of gut commensal microbiota and metabolic disorders in aquatic animals, is summarised. Albeit the probiotic C. butyricum has been applied in aquaculture, and further research, such as the inter‐relationships between the probiotic and the immune system of the host and the possible application of C. butyricum as an alternative to antibiotic substitution for the prevention and treatment of diseases, is required for future detailed studies.

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In Vitro Protective Efficacy of Clostridium butyricum Against Fish Pathogen Infections

Cited by 31 publications

References 21 publications

Changes in the Intestine Microbial, Digestive, and Immune-Related Genes of Litopenaeus vannamei in Response to Dietary Probiotic Clostridium butyricum Supplementation

Changes in the Intestine Microbial, Digestive, and Immune-Related Genes of Litopenaeus vannamei in Response to Dietary Probiotic Clostridium butyricum Supplementation

Effects of dietary supplementation of probiotics on the growth, activities of digestive and non-specific immune enzymes in hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀)

Clostridium butyricum: a promising probiotic confers positive health benefits in aquatic animals

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