In vitro propagation, restriction fragment length polymorphism, and random amplified polymorphic DNA analyses of Angelica plants

Watanabe, Aki; Araki, Shigeru; Kobari, Shinichi; Sudo, Hiroshi; Tsuchida, Tsutomu; Uno, Takaya; Kosaka, Naoharu; Shimomura, Koichiro; Yamazaki, Mami; Saito, Kazuki

doi:10.1007/s002990050554

Cited by 32 publications

(15 citation statements)

References 9 publications

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“…M, molecular size marker (l v Hd III + ERI 50 ng ml 21 ). Festuca pratensis (Valles et al, 1993), rose (Matsumoto and Fukui, 1996), Norway spruce (Fourre Â et al, 1997), Pinus thunbergii (Goto et al, 1998), and Angelica acutiloba (Watanabe et al, 1998), which supports our findings. In contrast, somaclonal variations were reported in Triticum aestivum (Brown et al, 1993), Populus deltoides (Rani et al, 1995), sugar beet (Munthali et al, 1996) and peach (Hashmi et al, 1997).…”

Section: Resultssupporting

confidence: 92%

“…This technique has been used for molecular characterization of tissue culture-raised plants such as Saccharum sp. (Chowdhury and Vasil, 1993), Festuca pratensis (Valles et al, 1993), and Angelica acutiloba (Watanabe et al, 1998). However, the technical complexity of performing RFLP analysis, relatively high costs, and the use of radioisotopes in the detection method are some of the disadvantages for its routine application.…”

Section: Introductionmentioning

confidence: 99%

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Establishment of genetic fidelity of In vitro-raised Lilium bulblets through rapd markers

Varshney

Lakshmikumaran

Srivastava

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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Random amplified polymorphic DNA (RAPD) markers were utilized for screening the clonal fidelity of in vitro-raised bulblets of Lilium sp. (Asiatic hybrids) produced through adventitious mode of propagation. The first set of 14 bulblets was randomly chosen from about 175 micropropagated bulblets regenerated from 1 Â 1 cm 2 bulbscale segments (explants) of cultivar`Gran Paradiso' after four subcultures (after 6 mo.). The second set of 15 bulblets was selected again randomly after 12 subcultures (after one and a half years) when the bulblets were to be taken out for transplantation. Of the 20 primers used to screen the samples, only 14 primers gave clear reproducible bands. The 14 primers produced a total of 163 (an average of 11.6 bands per primer) scorable bands. Analysis of individual primers revealed the RAPD patterns produced were all shared by both the in vitro-raised bulblets (randomly selected after four and 12 subcultures) and the mother bulb. There was no variation observed within the tissue culture-raised progenies. Thus, our results show that adventitiously propagated in vitro bulblets of Asiatic hybrids of lilies are clonally uniform and stable.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Establishment of genetic fidelity of In vitro-raised Lilium bulblets through rapd markers

Varshney

Lakshmikumaran

Srivastava

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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“…insertions, deletions, inversions) (Williams et al 1990). Using the RAPD technique, the presence of genetic variations in micropropagated plants arose from axillary bud explants have been reported (Hashmi et al 1997;Watanabe et al 1998;Bindiya and Kanwar 2003). On the other hand, several reports have been published, where no somaclonal variation could be detected using molecular markers (Rani and Raina 2000).…”

Section: Growth Of Excised Roots and De Novo Shoot Regeneration In LImentioning

confidence: 96%

In vitro manipulations in St. John’s wort (Hypericum perforatum L.) for incessant and scale up micropropagation using adventitious roots in liquid medium and assessment of clonal fidelity using RAPD analysis

Goel

Kukreja

Bisht

2008

Plant Cell Tiss Organ Cult

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The present study describes the potential of in vitro grown adventitious roots of Hypericum perforatum L. commonly known as St. John's wort at low nutrient and auxin levels in the liquid medium for micropropagation. Roots were regenerated from shoot-derived callus on MS medium containing 4.0 mg l -1 Indole-3 acetic acid (IAA). IAA and Indole-3 butyric acid (IBA) were equally effective for the induction of roots from shoot cultures. Half strength MS medium containing 1.0 mg l -1 IAA was most found suitable for culturing roots in liquid medium. A total biomass of 4.13 ± 0.67 g comprising 226 ± 34.4 shoots and shoot buds along with roots was obtained per culture starting with 200 mg roots inoculum. Pretreatment with kinetin (2.0 mg l -1 ) enhanced the shoot multiplication. Shoots proliferated profusely from excised roots in static liquid medium supported with glass bead matrix. Growtek TM vessel was found suitable and cost effective system for high throughput plantlet production. In vitro grown roots regardless of their source of origin were an excellent and easy to handle source of explant for aseptic production of plantlets without loosing the morphogenetic potential over the generations. The plants exhibited 84-99% similarity among themselves through RAPD. The in vitro shoots produced can either be multiplied or rooted perpetually, and alternatively they can also be explored for the in vitro production of hypericin and hyperforin.

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“…But the genetic fidelity of a culture needs to be determined by molecular markers, such as restriction fragment length polymorphism (RFLP), random-amplified polymorphic DNA (RAPD), and amplified fragment length polymorphism (AFLP), simple sequence repeat (SSR), which are powerful tools in genetic identification. They have been used for confirming somatic hybrids (Naoki et al 1994), and genetically analyzing micropropagated plantlets (Watanabe et al 1998), genetic homogeneity through somatic organogenesis (Tang 2001), and genomic variations through somatic embryogenesis (Leroy et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Factors influencing in vitro regeneration from protoplasts of wild cotton (G. klotzschianum A) and RAPD analysis of regenerated plantlets

et al. 2005

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Plants of a diploid wild cotton species (G. klotzschianum A.) were efficiently regenerated from protoplasts isolated from immature somatic embryos and suspension cultures by studying various factors affecting regeneration. Purified protoplasts were cultured with the density of 2 -10 · 10 5 ml À1 , and the medium was k 3 inorganic salts with modified KM 8 P organic compositions, supplemented with several combinations of PGRs. Calluses were formed from protoplasts of suspension cultures and immature somatic embryos. The influences of carbon sources and GA 3 on callus differentiation and somatic embryo germination were analyzed. Somatic embryos germinated normally and formed regenerated plantlets. Regenerated plantlets were transferred to the soil and seeds were obtained. Random amplified polymorphic DNA (RAPD) analysis using 80 arbitrary oligonucleotide 10-mers showed 23 primers that gave 74 clear reproducible bands, with amplification products being monomorphic for 14 tested plantlets. A total of 1036 bands obtained exhibited no aberration in RAPD banding patterns in the 14 plants. Plants regenerated via somatic embryogenesis from the diploid cotton protoplasts have genetic homogeneity. Abbreviations: 2,4-D -2,4-Dichlorophenoxyacetic acid; GA 3 -Gibberellic acid; DMRT -Duncan's multiple range test; FDA -Fluorescein Diacetate; IAA -Indole acetic acid; IBA -Indole-3-butyric acid; MSB -MS (Murashige & Skoog 1962) medium plus B 5 (Gamborg et al. 1968) vitamins; NAA aNaphthylene acetic acid; PGR -Plant growth regulator Plant Growth Regulation (2005) 46:79-86 Ó Springer 2005

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In vitro propagation, restriction fragment length polymorphism, and random amplified polymorphic DNA analyses of Angelica plants

Cited by 32 publications

References 9 publications

Establishment of genetic fidelity of In vitro-raised Lilium bulblets through rapd markers

Establishment of genetic fidelity of In vitro-raised Lilium bulblets through rapd markers

In vitro manipulations in St. John’s wort (Hypericum perforatum L.) for incessant and scale up micropropagation using adventitious roots in liquid medium and assessment of clonal fidelity using RAPD analysis

Factors influencing in vitro regeneration from protoplasts of wild cotton (G. klotzschianum A) and RAPD analysis of regenerated plantlets

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