In Vitro Propagation of Three MXM (Prunus Avium X P. Mahaleb) Cherry Rootstocks

Zilkah, S.; Faingersh, E.; Rotbaum, A.

doi:10.17660/actahortic.1992.314.10

Cited by 13 publications

(4 citation statements)

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“…In the absence of BA, meristems failed to develop and died within the first 6 wk of culture. Usually, different genotypes will behave very differently in vitro, as has been demonstrated by Snir (1982) working with seven different cuhivars of sweet cherry or Zilkah et al (1992) with three selections from an interspecific cross between Prunus avium and Prunus mahaleb. Results in our study with four different apricot cuhivars also showed large differences among them.…”

Section: ~ 5omentioning

confidence: 95%

Introduction and establishment of apricot in vitro through regeneration of shoots from meristem tips

Pérez‐Tornero

Burgos

Egea

1999

In Vitro Cell.Dev.Biol.-Plant

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In this study different aspects of the in vitro introduction and establishment of apricot cuhivars were investigated through meristem tip culture. The best time to introduce the meristems of 'Canino' was when buds were starting to swell. Various plant growth regulators were used at different concentrations on four distinct apricot cultivars to promote the development of the meristems to shoots which could then be micropropagated. Very diverse results were obtained depending on the genotype. In general, meristems did not survive without N6-benzyladenine. Concentrations of gibberellic acid from 2 to 4 mg 1-' (5.8-11.4 p.M) promoted explant elongation. This step was critically important to obtain apricot shoots large enough to be transferred to proliferation medium.

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Section: ~ 5omentioning

confidence: 95%

Introduction and establishment of apricot in vitro through regeneration of shoots from meristem tips

Pérez‐Tornero

Burgos

Egea

1999

In Vitro Cell.Dev.Biol.-Plant

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“…After the multiplication phase, in the previously described treatment, Bošnjaković et al (2013) rootstock selections were placed on DKW hormone-free medium, aimed at improving shoot elongation before rooting. Although some authors reported achieving shoot elongation prior to rooting in the treatments based on BA lower levels (Zilkah et al, 1992;Szczygiel and Wojda, 2010), any improvements in shoot elongation could not observe (unpublished data). However, DKW hormone-free medium had a positive influence on the elongation of multiplied shoots, in line with Pruski et al (2005).…”

Section: Plant Materials and Culture Establishmentmentioning

confidence: 99%

Rapid Propagation of Sweet and Sour Cherry Rootstocks

Doric

Ognjanov

Ljubojević

et al. 2014

Not Bot Horti Agrobo

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The paper presents a protocol for micropropagation of Prunus sp. rootstocks included in the sweet and sour cherry breeding program. Germplasm diversity for rootstock breeding derives from natural populations, where conditions and biological vectors for systematic infection with viral diseases are constantly present. The establishment of aseptic culture depends primarily on the explant type, as all selections were collected from natural habitat. For nearly all investigated selections, dormant buds were the favored source, due to enabling rosette initiation in more than 58% cases. In P. cerasus L. selections, 100% contamination was noted when shoot tips were used as an explant source. Significant influence of the double-phase medium on the number and height of multiplied shoots was observed in the standard cherry rootstock, â€˜Gisela 6â€™. For P. fruticosa Pall., selection â€˜SV1â€™ and â€˜SV2â€™, and P. cerasus â€˜D6â€™ selection, the double-phase medium also had a significant effect on the height of multiplied shoots, when compared to solid DKW (Driver and Kuniyuki Walnut) medium. Genetic variability of selections within the investigated species resulted in variable plant rooting success. Adding Fe-EDDHA (Ethylenediamine di-2-hydroxy-phenyl acetate ferric) in the 200 mg l-1 concentration to the rooting medium significantly enhanced the percentage of rooted plants. The highest rooting percentage was noted for â€˜Gisela 6â€™ and â€˜D6â€™ genotype at 1 mgl-1 IBA (indole-3-butyric acid), while 0.8 mgl-1 was the optimum concentration for P. mahaleb L. â€˜M1â€™ selection. P. fruticosa genotypes required significantly higher IBA concentration for rooting (2.5 and 3.5 mg l-1).

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“…Bu nedenle her genotip için ayrı çalışmaların yapılma zorunluluğu vardır. Örneğin Zilkah et al (1992), MxM (Prunus avium x Prunus mahaleb) kiraz anaçlarının üç klonunun in vitro üretimi üzerine yaptıkları çalışmada, MxM 2 ve MxM 60 klonları için Boxus ortamının, MxM 46 klonu için ise, Tabachnik ve Kester ortamının en iyi sonuç verdiğini saptamışlardır. Sürgün çoğaltımında ise, Almehdi ve Prfitt (AP) ortamına MxM 2 klonu için 0,2 mg/l BA ve 0,01mg/l IBA ilavesiyle en iyi sonuç alındığı tespit edilirken, MxM 46 klonunda 6 mg/l BA ve 0,01 mg/l IBA, M×M 60 klonunda ise 0,5 mg/l BA, 0,2 mg/l GA 3 ve 0,01mg/l IBA ilave edilmesi durumunda en iyi başarı elde edilmiştir.…”

Section: B Iunclassified

Bir İdris Anacı ‘Pontaleb’in Doku Kültürü İle Çoğaltılma Olanaklarının Araştırılması

Zainel¹,

Hepaksoy²

2018

Ege Üniversitesi Ziraat Fakültesi Dergisi

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ÖZET u çalışmada, kiraz için önemli bir anaç olan idris popülasyonu içinden Fransa'da seçilen 'Pontaleb' tohum anacının doku kültüründe vejetatif olarak çoğaltılabilme olanağı araştırılmıştır. Sürgün uçları alındıktan sonra yüzey sterilizasyonu yapılarak dikim yapıldı. Murashige Skoog (MS) temel besin ortamına, bitki büyüme düzenleyicisi olarak 1-2 mg/l BAP, 0,1 mg/l IBA/NAA ile 0,1 veya 0,5 mg/l GA3 eklenmiştir. Denenen ortamlar içinde sürgün gelişimi ve çoğalması dikkate alındığında 2 mg/l BAP + 0,1 mg/l NAA + 0,5 mg/l GA3 içeren MS ortamı diğerlerine göre daha başarılı bulunmakla birlikte çalışmada tatminkar sonuçlar elde edilememiştir. ABSTRACT n this study, the possibility of vegetative propagation by tissue culture of 'Pontaleb' seedling rootstock selected from the mahaleb population in France which is an important rootstock for sweetcherry was investigated. Shoot tip explants was taken and then surface sterilized and planted. Murashige Skoog (MS) medium supplemented with 1 and 2 mg/l BAP, 0,1 mg/l IBA or NAA and GA3 were used. For proliferation and multiplication, MS medium containing 2 mg/l BAP + 0,1 mg/l NAA + 0,5 mg/l GA3 was found to be more succesful than the others but satisfactory results were not obtained in the study.

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In Vitro Propagation of Three MXM (Prunus Avium X P. Mahaleb) Cherry Rootstocks

Cited by 13 publications

References 0 publications

Introduction and establishment of apricot in vitro through regeneration of shoots from meristem tips

Introduction and establishment of apricot in vitro through regeneration of shoots from meristem tips

Rapid Propagation of Sweet and Sour Cherry Rootstocks

Bir İdris Anacı ‘Pontaleb’in Doku Kültürü İle Çoğaltılma Olanaklarının Araştırılması

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