In vitro propagation of the endangered date palm (Phoenix dactylifera L.) cv. Ghazal: 2- Improvement of germination and development of embryos by silver nitrate and silver thiosulfate

Diab, Mohamed I.

doi:10.9790/264x-03046674

Cited by 6 publications

(7 citation statements)

References 18 publications

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“…The STS at 0.8 mM exceeded the other concentrations to fold the values to 2.8 and 3.1 with 1.2 and 2 mM STS, respectively. This may be referring to that the increment of STS concentrations negatively affected the fresh weight of microtuber due to the toxicity of silver ion in high concentrations and this agree with Ibrahim et al (2016) and Diab (2017). In 'Santana', highest value of F.W.…”

Section: Effect Of Silver Thiosulphate (Sts) Concentrations On Numbersupporting

confidence: 71%

“…The obtained value with 0.4 mM STS was folded 1.7, 1.8, 1.6 and 2.0 times as compared with the values with 0.8, 1.2, 1.6 and 2 mM STS, respectively. This may be referring to that the increment of STS concentrations negatively affected the number of microtubers/plantlet due to the toxicity of silver ion in high concentrations and this agree with Ibrahim et al, (2016) and Diab (2017). No significant differences were detected among all concentrations in Santana.…”

Section: Effect Of Silver Thiosulphate (Sts) Concentrations On Numbersupporting

confidence: 65%

“…These results agree with those of Ibrahim et al (2016), who mentioned that the increase of STS at medium to 1.5ml/l decreased the potato growth. In addition Diab (2017), who observed that the increasing STS concentrations from 0.5 to 8 ml/l decreased gradually root number and their length of date palm.…”

Section: Resultsmentioning

confidence: 96%

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Effect of Silver Thiosulphate and Photoperiod on in Vitro Tuberization of Three Potato (Solanum Tuberosum L.) Cultivars

El-Sayed¹,

Taha²,

Darwish³

et al. 2021

PLANT ARCHIVES

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The experiment was carried out to study the effect of 6 concentrations of silver thiosulphate (STS) and photoperiod on in vitro tuberization of three potato cultivars, namely, Herms, Spunta and Santana. The experiment was conducted in tissue culture laboratory, Vegetable Crops Dep., Fac. Agric., Cairo Univ. Tip meristems in each cultivar were excised and cultured on MS media. In vitro plantlets of all cultivars were multiplied as per routine by sub culturing twice. In microtuberization stage, nodal cuttings from second subculture were cultured on MS media with 0, 0.4, 0.8, 1.2, 1.6 or 2 mM of STS. The treatments were kept under dark or light conditions. STS at 0.4 and 0.8 mM recorded the tallest microplants. The highest number of leaves and branches per plantlet were obtained with 0.4mM STS. On the other hand, the STS at 2 mM recorded the lowest values of plantlet height, number of leaves and branches per plantlet. The highest percentage of microtuberization was recorded with STS at 0.4 mM under dark conditions, and at 2 mM under light one. At dark, Spunta with 1.6 mM STS produced significantly the highest number of microtubers/plantlet. Whereas at light, the maximum number of microtubers/plantlet was obtained with 0.4, 0.8 and 1.2 mM STS in Herms, Spunta and Santans, respectively. In'Spunta', application of STS at 0.4 mM under light folded the number of microtubers/plantlet 2.9 compared with dark. In Santana, number of microtubers/plantlet of 0, 0.8, 1.2, 1.6 and 2 mM STS were higher in light comparing to dark. STS applied at 0.8 and 1.2 mM produced significantly the highest value of microtuber fresh weight in Spunta and Santana, respectively under dark. In 'Spunta', the applications with 0, 1.2, 1.6 and 2 mM STS at light conditions recorded fresh weight of microtuber higher than in dark.

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Section: Effect Of Silver Thiosulphate (Sts) Concentrations On Numbersupporting

confidence: 71%

Section: Effect Of Silver Thiosulphate (Sts) Concentrations On Numbersupporting

confidence: 65%

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Effect of Silver Thiosulphate and Photoperiod on in Vitro Tuberization of Three Potato (Solanum Tuberosum L.) Cultivars

El-Sayed¹,

Taha²,

Darwish³

et al. 2021

PLANT ARCHIVES

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“…CuSO 4 and AgNO 3 treatments have promoted embryogenic callus and plant regeneration in many plant species (Purnhauser and Gyulai 1993;Nirwan and Kothari 2003;Kothari et al 2004;Joshi and Kothari 2007). Embryogenic callus formation, proliferation, embryos differentiation and germination were in uenced by AgNO 3 amendment in the cultivation medium of Picea glauca (Kang and Yeung 1995), Hedychium muluense (Sakhanokho et al 2009), Phoenix dactylifera (Diab 2017;Roshanfekrra et al 2017;Abdolvand et al 2018) and Coffea species in genotype-dependent manner (Fuentes et al 2000;Giridhar et al 2004;Lope-Gomez et al 2016;Rojas-Lorz et al 2019) while it in uenced shoot morphogenesis in Brassica napus (Uliaie et al 2008;Cristea et al 2012). AgNO 3 at 10 mg L −1 improved the frequency of embryogenic callus formation from immature embryo-induced callus cultures of wheat ).…”

Section: Indirect Somatic Embryogenesismentioning

confidence: 99%

Influence of silver nitrate and copper sulphate on somatic embryogenesis, shoot morphogenesis, multiplication and associated physiological biochemical changes in Gladiolus hybridus L.

Isah

Qurratul

Umar

2022

Preprint

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Micronutrients are essential for in vitro plant tissue growth, and studying their culture medium levels manipulation to promote growth and morphogenesis is essential in plant tissue culture experiments. In the present study, influence of CuSO4.5H2O (0–15 µM), AgNO3 (0–60 µM) and their combinations on callus induction and various morphogenic processes at different treatment levels were studied in G. hybridus. AgNO3 was more efficient in promoting the parameters studied, with 40 µM been more efficient over other treatment levels tested. In the case of CuSO4.5H2O treatments, maximum efficiency was achieved with 7.5 µM over other concentration treatment of the tissues analysed. Results of the experiments on morphogenesis and associated physiological biochemical changes suggests that combined treatment with the two metals promoted morphogenesis at higher levels through influence on physiological biochemical changes essential for the morphogenic parameters studied. Application of the technique on embryogenic tissue formation and somatic embryos differentiation seems of appropriate application when using metals for the experiment.

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“…Silver nitrate (AgNO 3 ) (Rojas-Lorz et al 2019), silver thiosulfate (Ag 2 S 2 O 3 ) (Diab 2017), and silver nanoparticles (AgNPs) (Malik et al 2021) are all added to in vitro culture media. Their primary function is to accelerate the callogenesis process and improve plant regeneration.…”

Section: Introductionmentioning

confidence: 99%

Effect of copper and silver ions on sequence and DNA methylation changes in triticale regenerants gained via somatic embryogenesis

Pachota

Orłowska

2022

J Appl Genetics

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Somatic embryogenesis is a plant regeneration method that can be exploited in tissue culture systems for a variety of tasks, such as genetic modification or the selection of somaclones with advantageous characteristics. Therefore, it is crucial to create efficient regeneration procedures and comprehend how medium components affect regeneration effectiveness or the degree of variation created in plant tissue cultures. The level of tissue culture-induced variation in triticale regenerants was examined in the current study in relation to the concentration of copper and silver ions in the induction media as well as the length of time immature zygotic embryo explants were incubated on these media. The high degree of variation (45%) revealed by the methylation-sensitive amplified fragment length polymorphism approach for estimating variation included 38% DNA sequence alterations, 6% DNA demethylation, and 1% de novo DNA methylation. Different levels of variance were found in relation to various DNA sequence settings. The CHG context had the most alterations, whereas CG experienced the fewest; sequence variation predominated in each sequence context. Lower copper ion concentrations showed the most variance. However, it could not be connected to the duration of in vitro culture or the effect of silver ions. Accordingly, we think that altering the concentration of copper ions in the induction medium may throw off the equilibrium of the metabolic processes in which copper is involved, resulting in tissue culture-induced variation.

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In vitro propagation of the endangered date palm (Phoenix dactylifera L.) cv. Ghazal: 2- Improvement of germination and development of embryos by silver nitrate and silver thiosulfate

Abstract: Date palm (Phoenix dactylifera L.) is one of the most importan tree crops in the deserts

Cited by 6 publications

References 18 publications

Effect of Silver Thiosulphate and Photoperiod on in Vitro Tuberization of Three Potato (Solanum Tuberosum L.) Cultivars

Effect of Silver Thiosulphate and Photoperiod on in Vitro Tuberization of Three Potato (Solanum Tuberosum L.) Cultivars

Influence of silver nitrate and copper sulphate on somatic embryogenesis, shoot morphogenesis, multiplication and associated physiological biochemical changes in Gladiolus hybridus L.

Effect of copper and silver ions on sequence and DNA methylation changes in triticale regenerants gained via somatic embryogenesis

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