In vitro propagation of the rare medicinal plant Ceropegia candelabrum L. through somatic embryogenesis

Abstract

SummaryEfficient in vitro propagation of Ceropegia candelabrum L. (Asclepidaceae) through somatic embryogenesis was established. Somatic embryogenesis depended on the type of plant growth regulators in the callus-inducing medium. Friable callus, developed from leaf and internode explants grown on Murashige and Skoog (MS) medium supplemented with 4.52 mM 2,4-dichlorophenoxyacetic acid (2,4-D), underwent somatic embryogenesis. Compared to solid media, suspension culture was superior and gave rise to a higher number of somatic embryos. Transfer of the friable callus developed on MS medium containing 4.52 mM 2,4-D to suspension cultures of half-or quarter-strength MS medium with lower levels of 2,4-D (0.23 or 0.45 mM) induced the highest number of somatic embryos, which developed up to the torpedo stage. Somatic embryogenesis was asynchronous with the dominance of globular embryos. About 100 mg of callus induced more than 500 embryos. Upon transfer to quarter-strength MS agar medium without growth regulators, 50% of the somatic embryos underwent maturation and developed into plantlets. Plantlets acclimatized under field conditions with 90% survival.

Regeneration of Shoots from Callus of Ceropegia pusilla Wight and ARN

“…bulbosa [18]. In Ceropegia candelabrum [19] and Decalepis hamiltonii [20] callus was produced from leaf and internodal explants. Later, same callus has produced somatic embryos too.…”

Improvement of somatic embryogenesis and plantlet conversion in Oplopanax elatus, an endangered medicinal woody plant

“…For many rare and endangered plant species, in vitro regeneration has been accomplished through organogenesis by using axillary bud and callus cultures (Negash 2002; Gomes et al 2003; Martin 2003a); however, somatic embryogenesis is not a commonly used method for such species (Augustine and D’ souza 1997; Beena and Martin 2003). In the present study, 200 normally converted young plantlets of Oplopanax elatus (about 7 to 10 cm in length) were selected and then transferred to an artificial soil mixture (Figure 6A–F).…”

In vitro conservation of Ceropegia elegans, an endemic plant of South India

“…The positive response of rooting in the present study is similar to observation in other Asclepiads and Caralluma spp. (Aruna et al, 2009), Caralluma edulis (Rathore et al, 2008), Ceropegia candelabrum (Beena and Martin, 2003), Ceropegia intermedia (Karuppusamy et al, 2009), Cryptolepis buchanani (Prasad et al, 2004), Decalapis arayalpatra (Gangaprasad et al, 2005;Sudha et al, 2005), Hoya wightii ssp. palniensis (Lakshmi et al, 2010), Leptadenia reticulata (Rathore et al, 2010) and Pergularia daemia (Kiranmai et al, 2008).…”