In vitro propagation of sweet potato (Ipomoea batatas (L.) Lam.) cultivars

Abubakar, Aminu Shehu; Yahaya, S. U.; Shaibu, Abdulwahab S.; Ibrahim, H.; Ibrahim, Aminu; Lawan, Z. M.; Isa, Adamu Mani

doi:10.18805/ag.d-128

Cited by 12 publications

(11 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Regeneration of shoots from calli in any of the two media (MS0.8 and LS0.8) tested could not be achieved, especially from the white and compacted calli obtained, indicating a strong varietal response to in vitro treatment. Similar results were noted by other workers where white or brown compact surface calli failed to regenerate shoots [17,18,19].…”

Section: Fig 2 Shoot Regeneration Potential Of Embryogenic Calli Onsupporting

confidence: 91%

Responses of Different Explants of Sweet Potato on Modified MS and LS Based Nutrient Media in vitro

Michael

2019

AJAAR

View full text Add to dashboard Cite

A good number of reports on sweet potato (Ipomoea batatas L.) tissue culture using different tissues of various cultivars with varying level of efficiency and reproducibility are available but callus induction and plantlet regeneration are recalcitrant and limited to a few genotypes in response to different treatments in vitro. Reported in this paper is a procedure in which several explants of three high yielding and drought tolerant sweet potato cultivars (SK010, WHCH005 and PRAP496), mostly grown and cultivated in the highlands of Papua New Guinea (PNG) were used to induce embryogenic callus and regenerate plantlets. To achieve a reliable and an efficient system for inducing embryogenic callus on stem, petiole and leaf disc explants, a modified form of Murashige and Skoog (MS) and Linsmaier and Skoog (LS) media, supplemented with 1 gL-1 picloram and 8 gL-1 agar was used. This procedure resulted in large amount of embryogenic calli that were potentially capable of regenerating whole plantlets on all the different types of explants tested. Further attempts made in plantlet regeneration failed, however ways in which improvement of the tested regeneration media can be made for plant regeneration in similar studies were discussed.

show abstract

Section: Fig 2 Shoot Regeneration Potential Of Embryogenic Calli Onsupporting

confidence: 91%

Responses of Different Explants of Sweet Potato on Modified MS and LS Based Nutrient Media in vitro

Michael

2019

AJAAR

View full text Add to dashboard Cite

show abstract

“…This variation, however, might not be unconnected to several factors such as genotype and its interaction with the growth environment, media composition, growth regulators, etc. (Abubakar et al, 2018;Pandey et al, 2003). The least percentage (14.29%) in callus induction was found in IAA (2.0 mg/L) + BAP (0.5 mg/L).…”

Section: Resultsmentioning

confidence: 91%

Sterilization procedure and callus regeneration in black turmeric (Curcuma caesia)

Abubakar

Pudake

2019

ASD

Self Cite

View full text Add to dashboard Cite

Sterilization procedure, media composition, explants selection and control of physical environment are critical for successful cultures and callus induction with surface sterilization being very challenging in most plants. Five different sterilization methods were evaluated to come up with the best for subsequent use to establish an in vitro regeneration method for the induction of callus in Curcuma caesia using excised leaf and rhizome explants. Murashige and Skoog (MS) media supplemented with various concentration of 2,4-Dichlorophenoxy acetic acid (2,4-D)/Indole-3-acetic acid (IAA) (0.5- 5.0mg/L), singly or in combination with Benzyl aminopurine (BAP)/Kinetin (KIN) (0.1-5.0mg/L), 0.3% sucrose and 0.08% agar were used. The result of the sterilization procedures showed 15% NaHClO3 (5min) + 70% Ethanol (30s) + 0.1% HgCl2 (5min) to be the most effective in controlling contamination in C. caesia among all the treatments tested. The response to callus induction was found to depend on the type of explants used and growth regulators combination. Leaf explants gave the highest percentage of callus induction. Highest percentage of callus induction (66.70%) was obtained in the growth regulator combination of 2, 4-D (0.5mg/L) + BAP (0.1mg/L) and least (14.29%) in IAA (2.0mg/L) + BAP (0.5mg/L). Equal and higher concentration of 2, 4-D + BAP of 5.0mg/L each also provided better result (40.00%). No callus was obtained in all the single concentration of 2, 4-D used.

show abstract

“…these horticultural crops are underutilised and neglected vegetables such as sweet potato and taro (Colocasia esculenta) [20] whose micropropagation and germplasm preservation have been successful [21][22][23][24][25]. In this study, the Livingstone potato was also subjected to micropropagation techniques, and the results are discussed here.…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Livingstone Potato (Plectranthus esculentus N.E.Br)

Kujeke

Chitendera

Masekesa

et al. 2020

Advances in Agriculture

View full text Add to dashboard Cite

Livingstone potato (Plectranthus esculentus N.E.Br) is an underutilised indigenous root vegetable grown by communal farmers in the eastern provinces of Zimbabwe. It is vegetatively propagated using unimproved retained tubers from the previous season. The risk of disease carryover is therefore high, leading to poor yields. The objective of the study was to exploit the tissue culture technique of micropropagation to produce a mass supply of healthy planting material for improved productivity. Two experiments were conducted: firstly, to determine the best explant type and secondly, to determine the best landrace and plant growth regulators for the growth of plantlets. The landraces, namely, Ndurwe, Musande, Chibanda, and Chizambezi, were sourced from communal farmers in the stated production areas. Naphthalene acetic acid (NAA) and benzyl amino purine (BAP) were the auxin and cytokinin used, respectively. The first experiment was laid out as a randomized complete block design (RCBD) with two factors: landrace and explant type (shoot tips, nodes, and leaves). After culturing the explants on a plain Murashige Skoog (MS) medium for ten weeks, the best explant was the node with regards to the number of nodes, shoots, and roots of the plantlets which were significant (P<0.05). The second experiment was laid out as a RCBD with two factors: landraces and the plant growth regulator combinations. The nodes were subcultured on an MS medium supplemented with the 16 combinations of plant growth regulators (0 mg/l, 0.5 mg/l, 1 mg/l, and 2 mg/l BAP concentrations: 0 mg/l, 0.2 mg/l, 0.5 mg/l, and 1 mg/l NAA concentrations), respectively. Chizambezi performed best and is, therefore, highly recommended for the rapid multiplication of Livingstone potato. Results from this study have clearly demonstrated that the addition of NAA: BAP at varying concentrations was significant and is essential for optimizing the growth media for micropropagation of Livingstone potato in Zimbabwe. Commercial production of plantlets can, therefore, be carried out to provide healthy planting material for the communal farmers for improved productivity while preserving the germplasm of the underutilised crop at the same time.

show abstract

In vitro propagation of sweet potato (Ipomoea batatas (L.) Lam.) cultivars

Cited by 12 publications

References 8 publications

Responses of Different Explants of Sweet Potato on Modified MS and LS Based Nutrient Media in vitro

Responses of Different Explants of Sweet Potato on Modified MS and LS Based Nutrient Media in vitro

Sterilization procedure and callus regeneration in black turmeric (Curcuma caesia)

Micropropagation of Livingstone Potato (Plectranthus esculentus N.E.Br)

Contact Info

Product

Resources

About