Responses of Different Explants of Sweet Potato on Modified MS and LS Based Nutrient Media in vitro

Abstract

A good number of reports on sweet potato (Ipomoea batatas L.) tissue culture using different tissues of various cultivars with varying level of efficiency and reproducibility are available but callus induction and plantlet regeneration are recalcitrant and limited to a few genotypes in response to different treatments in vitro. Reported in this paper is a procedure in which several explants of three high yielding and drought tolerant sweet potato cultivars (SK010, WHCH005 and PRAP496), mostly grown and cultivated in the highlands of Papua New Guinea (PNG) were used to induce embryogenic callus and regenerate plantlets. To achieve a reliable and an efficient system for inducing embryogenic callus on stem, petiole and leaf disc explants, a modified form of Murashige and Skoog (MS) and Linsmaier and Skoog (LS) media, supplemented with 1 gL-1 picloram and 8 gL-1 agar was used. This procedure resulted in large amount of embryogenic calli that were potentially capable of regenerating whole plantlets on all the different types of explants tested. Further attempts made in plantlet regeneration failed, however ways in which improvement of the tested regeneration media can be made for plant regeneration in similar studies were discussed.

Sweet potato is a strategic root crop in Oceania: A synthesis of the past research and future direction

“…Due to the genomic complexity and incompatibilities hindering conventional genetic improvement, researchers have used several available modern technologies to advance the efficacy of cultivar development (Tanaka et al, 2017). Attempts in the use of genetic transformation technologies (particle bombardment, electrophoresis, Agrobacterium tumefaciens, and A. rhizogenes mediated transformation; random amplified polymorphic DNA, amplified fragment length polymorphism, simple sequence repeats) to transfer genes have been made (Chen et al, 2013) and procedures have been developed (Michael, 2019a;Michael, 2019b). Genome editing has been studied to improve agronomic traits, nutritional quality, and resistance to abiotic and biotic stresses (Lu et al, 2017;Zhan et al, 2021).…”