In Vitro Propagation of Sweet Potato1

Litz, R. E.; Conover, R. A.

doi:10.21273/hortsci.13.6.659

Cited by 18 publications

(4 citation statements)

References 3 publications

(3 reference statements)

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“…Sweet potato, a vegetatively propagated root crop, is an important staple of subsis tence farmers in tropical areas (4). Clonal propagation within these areas is achieved routinely by vine cuttings, a method ineffec tive for maintaining pathogen-free stock plants (7) and resulting in the dissemination of dis eased or pest-infested planting materials. Ad d itio n a lly , sw eet potato germ plasm conservation involves the maintenance of field collections that is costly and may result in the loss of desirable genotypes through fac tors such as natural disasters and attack by pathogens.…”

Section: Somatic Embryogenesis In Sweet Potatomentioning

confidence: 99%

“…For ex ample, the application of thermotherapy in conjunction with meristem culture has ena bled virus elimination from various clones (5). Propagation from adventitious shoots (2, 12), cultured shoot apices (7), and em bryogenic anther callus cultures (10) has been reported. Furthermore, limited-growth shoot tip cultures are being used for germplasm storage (3).…”

Section: Somatic Embryogenesis In Sweet Potatomentioning

confidence: 99%

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Somatic Embryogenesis in Sweet Potato

Jarret¹,

Salazar²

1984

horts

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Asexual embryos arose from callus derived from axillary bud shoot tips of 6 sweet potato [Ipomoea batatas (L.) Lam.] plant introductions (PIs) when cultured on a modified Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D). Transferral of embryogenic callus to auxin-free medium resulted in the continued development and eventual germination of individual somatic embryos and recovery of rooted plantlets.

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Section: Somatic Embryogenesis In Sweet Potatomentioning

confidence: 99%

Section: Somatic Embryogenesis In Sweet Potatomentioning

confidence: 99%

Somatic Embryogenesis in Sweet Potato

Jarret¹,

Salazar²

1984

horts

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“…Later, investigations continued to assess the effect of plant growth regulators on in vitro growth and development of sweetpotato however these reports generally only included one to four sweetpotato genotypes. In many of the studies callus formation and/or other genotype-specific responses to plant growth regulators were observed (Fadaladeen et al, 2022;Beyene et al, 2020;Alula et al, 2018;Sefasi et al, 2013;Dugassa and Feyissa, 2011;Jarret et al, 1991a;Jarret et al, 1984;Litz and Conover, 1978). It was further reported that supplementing culture media with auxins and cytokinin could induce somaclonal variation in the plants, particularly during indirect organogenesis (i.e., callus formation) [Leva et al, 2012;Bairu et al, 2011Bairu et al, , 2006.…”

Section: Introductionmentioning

confidence: 99%

Thiamine improves in vitro propagation of sweetpotato [Ipomoea batatas (L.) Lam.] – confirmed with a wide range of genotypes

Vollmer

Espirilla

Sánchez

et al. 2022

Plant Cell Tiss Organ Cult

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In vitro propagation of Plant Genetic Resources is a basic step for routine genebank and biotechnology research activities. Accelerating growth and rooting of in vitro plants contributes to an improvement in process efficiency and plant quality. In the present study the effect of supplemental thiamine and explant size on biometric variables, ion content in plant sap, chlorophyll content in leaves and moisture content in plants were assessed in a replicated trial on a group of seven in vitro sweetpotato accessions and validated in a set of other 45 accessions. It was shown that adding 0.1 mg L −1 of thiamine to modified Murashige and Skoog culture medium significantly increased plant height, root length, and number of nodes of in vitro sweetpotato shoot culture plants. No significant differences were observed for N03−, K+, Na+ and Ca++—ion content in plant sap, nor in leaf area, chlorophyll, or moisture content between plants grown with or without thiamine. Uninodal stem segments showed on thiamine-free medium a significantly lower root and plant growth, and reduced number of nodes, than plants grown from uni- and binodal segments on thiamine-supplemented medium. A subsequent experiment tested all the parameters above in a non-replicated screen with a set of 45 diverse sweetpotato accessions. With this diverse set of germplasm, the average plant and root length increased by 41 and 51%, respectively on thiamine-supplemented culture medium compared to the control treatment, confirming that supplemental thiamine is generally beneficial to sweetpotato in vitro shoot culture.

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“…Later, investigations continued to assess the effect of plant growth regulators on in vitro growth and development of sweetpotato however these reports generally only included two to four sweetpotato genotypes. In many of the studies callus formation and/or other genotype-speci c responses to plant growth regulators was observed (Alula et al, 2018;Sefasi et al, 2013;Jarret et al, 1984;Jarret et al, 1991a;Litz and Conover, 1978). It was further reported that supplementing culture media with auxins and cytokinin could induce somaclonal variation in the plants, particularly during indirect organogenesis (i.e., callus formation) [Leva et al, 2012;Bairu et al, 2011Bairu et al, , 2006.…”

Section: Introductionmentioning

confidence: 99%

Improved in vitro propagation of sweetpotato [Ipomoea batatas (L.) Lam.] – Confirmed with a wide range of genotypes

Vollmer

Espirilla

Sanchez

et al. 2022

Preprint

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In vitro propagation of Plant Genetic Resources is a basic step for routine genebank and biotechnology research activities. Accelerating growth and rooting of in vitro plants contributes to an improvement in process efficiency and plant quality. In the present study the effect of supplemental thiamine and explant size on biometric variables, ion content in plant sap, chlorophyll content in leaves and moisture content in plants were assessed in a replicated trial on a group of seven in vitro sweetpotato accessions and validated in a set of other 45 accessions. It was shown that adding 0.1 mg L -1 of thiamine to modified Murashige and Skoog culture medium significantly increased plant height, root length, and number of nodes of in vitro sweetpotato shoot culture plants. No significant differences were observed for N0 3 - , K + , Na + and Ca ++ - ion content in plant sap, nor in leaf area, chlorophyll, or moisture content between plants grown with or without thiamine. Uninodal stem segments showed on thiamine-free medium a significantly lower root and plant growth, and reduced number of nodes, than plants grown from uni- and binodal segments on thiamine-supplemented medium. A subsequent experiment tested all the parameters above in a non-replicated screen with a set of 45 diverse sweetpotato accessions. With this diverse set of germplasm, the average plant and root length increased by 41 and 51%, respectively on thiamine-supplemented culture medium compared to the control treatment, confirming that supplemental thiamine is generally beneficial to sweetpotato in vitro shoot culture.

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In Vitro Propagation of Sweet Potato1

Cited by 18 publications

References 3 publications

Somatic Embryogenesis in Sweet Potato

Somatic Embryogenesis in Sweet Potato

Thiamine improves in vitro propagation of sweetpotato [Ipomoea batatas (L.) Lam.] – confirmed with a wide range of genotypes

Improved in vitro propagation of sweetpotato [Ipomoea batatas (L.) Lam.] – Confirmed with a wide range of genotypes

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