Somatic Embryogenesis in Sweet Potato

Jarret, Robert L.; Salazar, Sergio Miguel

doi:10.21273/hortsci.19.3.397

Cited by 33 publications

(3 citation statements)

References 4 publications

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“…Later, investigations continued to assess the effect of plant growth regulators on in vitro growth and development of sweetpotato however these reports generally only included two to four sweetpotato genotypes. In many of the studies callus formation and/or other genotype-speci c responses to plant growth regulators was observed (Alula et al, 2018;Sefasi et al, 2013;Jarret et al, 1984;Jarret et al, 1991a;Litz and Conover, 1978). It was further reported that supplementing culture media with auxins and cytokinin could induce somaclonal variation in the plants, particularly during indirect organogenesis (i.e., callus formation) [Leva et al, 2012;Bairu et al, 2011Bairu et al, , 2006.…”

Section: Introductionmentioning

confidence: 99%

Improved in vitro propagation of sweetpotato [Ipomoea batatas (L.) Lam.] – Confirmed with a wide range of genotypes

Vollmer

Espirilla

Sanchez

et al. 2022

Preprint

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In vitro propagation of Plant Genetic Resources is a basic step for routine genebank and biotechnology research activities. Accelerating growth and rooting of in vitro plants contributes to an improvement in process efficiency and plant quality. In the present study the effect of supplemental thiamine and explant size on biometric variables, ion content in plant sap, chlorophyll content in leaves and moisture content in plants were assessed in a replicated trial on a group of seven in vitro sweetpotato accessions and validated in a set of other 45 accessions. It was shown that adding 0.1 mg L -1 of thiamine to modified Murashige and Skoog culture medium significantly increased plant height, root length, and number of nodes of in vitro sweetpotato shoot culture plants. No significant differences were observed for N0 3 - , K + , Na + and Ca ++ - ion content in plant sap, nor in leaf area, chlorophyll, or moisture content between plants grown with or without thiamine. Uninodal stem segments showed on thiamine-free medium a significantly lower root and plant growth, and reduced number of nodes, than plants grown from uni- and binodal segments on thiamine-supplemented medium. A subsequent experiment tested all the parameters above in a non-replicated screen with a set of 45 diverse sweetpotato accessions. With this diverse set of germplasm, the average plant and root length increased by 41 and 51%, respectively on thiamine-supplemented culture medium compared to the control treatment, confirming that supplemental thiamine is generally beneficial to sweetpotato in vitro shoot culture.

show abstract

Section: Introductionmentioning

confidence: 99%

Improved in vitro propagation of sweetpotato [Ipomoea batatas (L.) Lam.] – Confirmed with a wide range of genotypes

Vollmer

Espirilla

Sanchez

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Later, investigations continued to assess the effect of plant growth regulators on in vitro growth and development of sweetpotato however these reports generally only included one to four sweetpotato genotypes. In many of the studies callus formation and/or other genotype-specific responses to plant growth regulators were observed (Fadaladeen et al, 2022;Beyene et al, 2020;Alula et al, 2018;Sefasi et al, 2013;Dugassa and Feyissa, 2011;Jarret et al, 1991a;Jarret et al, 1984;Litz and Conover, 1978). It was further reported that supplementing culture media with auxins and cytokinin could induce somaclonal variation in the plants, particularly during indirect organogenesis (i.e., callus formation) [Leva et al, 2012;Bairu et al, 2011Bairu et al, , 2006.…”

Section: Introductionmentioning

confidence: 99%

Thiamine improves in vitro propagation of sweetpotato [Ipomoea batatas (L.) Lam.] – confirmed with a wide range of genotypes

Vollmer

Espirilla

Sánchez

et al. 2022

Plant Cell Tiss Organ Cult

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In vitro propagation of Plant Genetic Resources is a basic step for routine genebank and biotechnology research activities. Accelerating growth and rooting of in vitro plants contributes to an improvement in process efficiency and plant quality. In the present study the effect of supplemental thiamine and explant size on biometric variables, ion content in plant sap, chlorophyll content in leaves and moisture content in plants were assessed in a replicated trial on a group of seven in vitro sweetpotato accessions and validated in a set of other 45 accessions. It was shown that adding 0.1 mg L −1 of thiamine to modified Murashige and Skoog culture medium significantly increased plant height, root length, and number of nodes of in vitro sweetpotato shoot culture plants. No significant differences were observed for N03−, K+, Na+ and Ca++—ion content in plant sap, nor in leaf area, chlorophyll, or moisture content between plants grown with or without thiamine. Uninodal stem segments showed on thiamine-free medium a significantly lower root and plant growth, and reduced number of nodes, than plants grown from uni- and binodal segments on thiamine-supplemented medium. A subsequent experiment tested all the parameters above in a non-replicated screen with a set of 45 diverse sweetpotato accessions. With this diverse set of germplasm, the average plant and root length increased by 41 and 51%, respectively on thiamine-supplemented culture medium compared to the control treatment, confirming that supplemental thiamine is generally beneficial to sweetpotato in vitro shoot culture.

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“…Among various systems of plant regeneration, somatic embryogenesis is highly desired, as the process regularly provides high multiplication rates and can effectively be maintained for a long time. Genotype has been shown to be a limiting factor in induc-tion of somatic embryogenesis, as many cultivars of sweet potato gave low or no embryogenic responses at all [9][10][11]. Rapid and repetitive plant regeneration via somatic embryogenesis was demonstrated in only one genotype of sweet potato, PI318846-3 [12].…”

Section: Introductionmentioning

confidence: 99%

Effect of genotype, gelling agent, and auxin on the induction of somatic embryogenesis in sweet potato (Ipomoea batatas Lam.)

Triqui

Guedira

Chlyah

et al. 2007

Comptes Rendus. Biologies

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Lateral buds of six cultivars of sweet potato were induced to form embryogenic callus in a culture medium solidified with two types of gelling agents, Agar or Gelrite, and supplemented with various concentrations of auxins, 2,4-D, 2,4,5-T and Picloram. Of the six cultivars screened, only three gave an embryogenic response. Best results with an average of 3.53% embryogenic response were obtained with the medium solidified with Agar, while in Gelrite only 0.45% of lateral buds gave rise to embryogenic callus. The interaction between the genotype and auxins was highly significant; particularly the optimal response was obtained with cv. Zho and 865 yielding 10.7 and 14.7% somatic embryogenesis, respectively, in the medium containing 2,4,5-T or Picloram. The plant conversion was dramatically improved by subculture of the embryogenic callus on the medium with the combination of 1 µM 2,4-D and 1 µM Kinetin or 5 µM ABA alone before transfer of mature embryos onto hormone-free medium. The embryogenic callus of sweet potato and its sustained ability to further regenerate plants have regularly been maintained for several years by frequent subculture in 5 µM 2,4,5-T or the combination of 10 µM 2,4-D and 1 µM BAP or kinetin. The embryo-derived plants seemed apparently genetically stable and similar to the hexaploid parental plants, based on morphological analysis and their ploidy level determined by using flow cytometry. To cite this article: Z.

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Somatic Embryogenesis in Sweet Potato

Cited by 33 publications

References 4 publications

Improved in vitro propagation of sweetpotato [Ipomoea batatas (L.) Lam.] – Confirmed with a wide range of genotypes

Improved in vitro propagation of sweetpotato [Ipomoea batatas (L.) Lam.] – Confirmed with a wide range of genotypes

Thiamine improves in vitro propagation of sweetpotato [Ipomoea batatas (L.) Lam.] – confirmed with a wide range of genotypes

Effect of genotype, gelling agent, and auxin on the induction of somatic embryogenesis in sweet potato (Ipomoea batatas Lam.)

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