In vitro Propagation of Rubia cordifolia Linn., A Medicinal Plant of the Western Ghats

Radha, R; Shereena, S.R.; Kajaria, Divya; Krishnan, P. N.; Seeni, S.

doi:10.3923/ijb.2011.90.96

Cited by 21 publications

(10 citation statements)

References 6 publications

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“…The present study confirms the significance of the cytokinins (BA or Kn) for the direct shoot regeneration in maize. These findings are similar to the reports described in other plants ( Adhatoda vasica and Adhatoda beddomei ) using split node explants [1], [14], [20].…”

Section: Resultssupporting

confidence: 92%

Efficient in vitro direct shoot organogenesis from seedling derived split node explants of maize (Zea mays L.)

Mushke

Yarra

Bulle

2016

Journal of Genetic Engineering and Biotechnology

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Maize is one of the important cereal crops around the world. An efficient and reproducible regeneration protocol via direct organogenesis has been established using split nodes as ex-plants derived from 7 to 10 day old in vitro grown seedlings. Surface sterilized maize seeds were germinated on MS medium lacking plant growth regulators. Nodal sections of 7–10 day old seedlings were isolated, split longitudinally into two halves and cultured on regeneration medium containing different concentrations of 6-benzyladenine (2.20, 4.40, 6.60, 8.80, 11.0 and 13.2 μM) or kinetin (2.32, 4.65, 6.97, 9.29, 11.6 and 13.9 μM). Inclusion of 8.80 μM BA into MS supplemented medium triggered a high frequency of regeneration response from split node explants with a maximum number of shoots (12.0 ± 1.15) and the highest shoot length (3.0 ± 0.73) was obtained directly (without an intervening callus phase) within 4 weeks of culture. Further shoot elongation was achieved on medium containing 4.40 μM BA. The elongated micro shoots were rooted on MS medium fortified with 1.97 μM indole-3-butyric acid. The regenerated plantlets with roots were successfully hardened on earthen pots after proper acclimatization under greenhouse conditions. This new efficient regeneration method provides a solid foundation for genetic manipulation of maize for biotic and abiotic stresses and to enhance the nutritional values.

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Section: Resultssupporting

confidence: 92%

Efficient in vitro direct shoot organogenesis from seedling derived split node explants of maize (Zea mays L.)

Mushke

Yarra

Bulle

2016

Journal of Genetic Engineering and Biotechnology

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“…Thus, there is an urgent need to restore the natural population of this plant and use an alternative pharmaceutical source without taking it directly from nature. The tissue culture method is an alternative to provide raw material to produce a medicinal compound to achieve great benefits in the pharmaceutical aspect (Radha et al 2011) and to select genotypes that have considerable amounts of bioactive compound (Cantelmo et al 2013). Generally, organ culture, cell suspension, and callus culture have been used to study secondary metabolite synthesis using the tissue culture method (George and Sherrington 1984;Smetanska 2008).…”

Section: Introductionmentioning

confidence: 99%

Effect of sucrose and plant growth regulators on callogenesis and preliminary secondary metabolic of different explant Myrmecodia tuberosa

et al. 2018

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Sari YP, Kusumawati E, Saleh C, Kustiawan W, Sukartingsih. 2018. Effect of sucrose on callogenesis and preliminary secondary metabolic of different explant (Myrmecodia tuberosa). Nusantara Bioscience 10: 183-192. Myrmecodia tuberosa Jack is a medicinal plant that contains bioactive compounds, such as flavonoids, tannins, tocopherols, phenols, and an abundance of minerals, that are useful as antioxidants. With the constant increases in popularity of the medicinal plant, the M. tuberosa is threatened by extinction if over-exploitation continues. Thus, the effort to conserve this plant is vital. Tissue culture is an alternative method to conserve and produce active compounds that are similar to those of the native ant nest plant with callus. The addition of certain compounds such as sucrose can affect the secondary metabolite content through in vitro plant or callus. The aim of this research was to find the explant sources (cotyledon, stem, tuber, and root), determine the best growth regulator to produce the callus, and evaluate the optimum sucrose concentration to enhance secondary metabolite production of the callus. The results showed that callus was obtained from all explant sources and all growth regulators. The best callus that was marked by a friable green and yellowish green callus was provided by cotyledon with the growth regulator of 2 mg⋅L-1 of 2.4-dichlorophenoxyacetic acid (2.4-D) and 2 mg⋅L-1 of kinetin. Calli treated with 30 g of sucrose resulted in the best secondary metabolites, containing alkaloids, phenols, flavonoids, saponins, and steroids.

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“…14,15 In vitro propagation trials conducted using nodal explants inoculated on MS basal medium supplemented with benzyl adenine (1 mg/L) and IAA (0.02 mg/L); produced maximum of 5.9 and 5.2 shoots per explants respectively, and maximum 8.9 number of roots with 6.4 cm length. 16 Indirect in vitro propagation protocol was established by inducing callus from leaf, internode, and node explants. Induction of 85% callus was reported on 2,4-Dichlorophenoxyacetic acid (2.5 mg/L) + NAA (2.0 mg/L) and maximum 1.9 number of shoots per culture were achieved on BAP (4 mg/L) and Adenine sulphate (5 mg/L).…”

Section: Discussionmentioning

confidence: 99%

Conservation of Manjishtha–Rubia cordifolia L. through Nodal Culture

Sinha¹,

Rao²

2017

Journal of Drug Research in Ayurvedic Sciences

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Aim: Manjishtha-Rubia cordifolia L. (family: Rubiaceae) is an important medicinal plant and used in various Ayurvedic formulations. Plant parts like roots, stems, leaves and fruits are being used to treat various respiratory and skin diseases. Manjistha is excessively collected from natural habitat and becoming rare and vulnerable in different parts of country. Therefore, it is decided to develop a systematic in vitro protocol for rapid multiplication of the plant. Materials and methods: Nodal segments collected from healthy, desease free plant were used as explants. Pretreated and surface sterilized nodal segments were implanted on to MS basal medium as well as MS fortified with different concentrations of plant growth regulators viz., BAP, TDZ, Kn, NAA, IAA, IBA singly or in combinations. Then, the cultures were incubated at 22°C ± 2°C for 8 hours photoperiod with light intensity of 3000 lux. Results: Maximum number of shoots (20-25) developed from the nodal segments inoculated on MS + TDZ (0.5 mg/l) + 0.1% PVP liquid medium. The best rooting (2-3 roots) were developed in MS + IBA (2 mg/l) in 8 to 14 days. Conclusion: The in vitro protocol developed would be beneficial to multiply the plants of R. cordifolia on large scale within the short period with low cost and to conserve the plant.

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In vitro Propagation of Rubia cordifolia Linn., A Medicinal Plant of the Western Ghats

Cited by 21 publications

References 6 publications

Efficient in vitro direct shoot organogenesis from seedling derived split node explants of maize (Zea mays L.)

Efficient in vitro direct shoot organogenesis from seedling derived split node explants of maize (Zea mays L.)

Effect of sucrose and plant growth regulators on callogenesis and preliminary secondary metabolic of different explant Myrmecodia tuberosa

Conservation of Manjishtha–Rubia cordifolia L. through Nodal Culture

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