In vitro propagation of northern red oak (Quercus rubra L.)

Abstract

In vitro propagation of northern red oak (Quercus rubra) shoots was successful from cotyledonary node explants excised from 8-wk-old in vitro grown seedlings. Initially, four shoots per explant were obtained on Murashige and Skoog (MS) medium supplemented with 4.4 μM 6-benzylaminopurine (BA), 0.45 μM thidiazuron (TDZ), and 500 mg l −1 casein hydrolysate (CH) with a regeneration frequency of 64.7% after 3 wk. Subculturing explants (after harvesting shoots) to fresh treatment medium significantly increased shoot bud regeneration (16.6 buds per explant), but the buds failed to develop into shoots. A higher percentage (73.3%) of the explants regenerated four shoots per explant on woody plant medium (WPM) supplemented with 4.4 μM BA, 0.29 μM gibberellic acid (GA 3 ), and 500 mg l −1 CH after 3 wk. Explants subcultured to fresh treatment medium after harvesting shoots significantly increased shoot regeneration (16 shoots per explant). Shoot elongation was achieved (4 cm) when shoots were excised and cultured on WPM supplemented with 0.44 μM BA and 0.29 μM GA 3 . In vitro regenerated shoots were rooted on WPM supplemented with 4.9 μM indole-3-butyric acid. A higher percentage regeneration response and shoot numbers per explant were recorded on WPM supplemented with BA and GA 3 , than on MS medium containing BA and TDZ. Lower concentrations of BA and GA 3 were required for shoot elongation and prevention of shoot tip necrosis. Each cotyledonary node yielded approximately 20 shoots within 12 wk. Rooted plantlets were successfully acclimatized.

Micropropagation of annatto (Bixa orellana L.) from mature tree and assessment of genetic fidelity of micropropagated plants with RAPD markers

“…However, further growth of these buds was arrested. This observation is in conformity with other reports on in vitro propagation of trees like Pterocarpus marsupium (Husain et al 2007) and Quercus rubra (Vengadesan and Pijut 2008). Inhibition of shoot elongation and shoot fasciations were recorded.…”

In vitro regeneration of Feizixiao litchi (Litchi chinensis Sonn.)

“…For example, in Clematis, enhanced frequency of shoot development and greater internode elongation resulted from the influence of GA 3 . Compared with other studies on shoot bud differentiation such as Quercus (Vengadesan and Pijut, 2009) and Ammopiltanthus mongolicus (Yang et al, 2014), in this paper, the tap root of litchi produced black spots (1.5 cm), which ultimately led to plant death. Thus, GA 3 promoted rooting, inhibited root growth, and suppressed lateral root growth.…”

Influencia de las auxinas sobre el enraizamiento in vitro de microtallos de Nothofagus glauca (Phil.) Krasser

“…La falta de raíces en el medio libre de hormonas se podría explicar debido a la ausencia de estructuras preformadas para la emergencia de primordios radiculares en la especie, lo que sí es posible encontrar en otras especies como Salix sp., Populus sp., Ribes nigrum L. o Fagus sylvatica L. (Hassing 1972, Fink 1982 En este contexto, un sistema radical de calidad, según Vengadesan & Pijut (2009), considera el número de raíces por microtallo o brote, longitud de las raíces y ausencia de formación de callo, características que determinan el rendimiento durante la fase de aclimatación. Por tanto, en este estudio se obtuvo un sistema radical de calidad utilizando bajas concentraciones de AIB, contrario a lo que ocurre, por ejemplo, en Nothofagus leoni Espinosa, donde concentraciones más elevadas de AIB inducen un mejor sistema radical (Martínez-Pastur & Arena 1999).…”