In vitro regeneration of Feizixiao litchi (Litchi chinensis Sonn.)

Guo, Wei; Huanling, L; Wang, Shujun; Sun, Jinhua; Zhang, Xinchun; Wang, Jiabao

doi:10.5897/ajb2015.15175

Cited by 9 publications

(4 citation statements)

References 15 publications

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“…Embryogenic calli (ECs) of 'Feizixiao' were initiated by anthers on M1 medium (Table 1), and the anthers were obtained from the Institute of Tropical Fruits, Hainan Academy of Agricultural Sciences, Hainan province, China. ECs were subcultured on M3 medium and M4 medium (Table 1), with the media alternated every 30 days [3].…”

Section: Callus Induction and Culturementioning

confidence: 99%

“…Calli were transferred to T3 medium (Table 1) [3] for embryo induction. Cultures at days 0, 5, 10, 15, and 20 were sampled, frozen in liquid N 2 , and stored at −80 • C for the analysis of AA contents.…”

Section: Msmentioning

confidence: 99%

“…Traditional breeding techniques have limitations such as long reproductive cycles, high costs, and low efficiency, while molecular breeding has advantages such as short breeding cycles and the rapid improvement of targeted traits, making it a promising approach for the selection and breeding of new litchi cultivars [2]. An efficient in vitro regeneration technology system is a prerequisite for molecular breeding, and somatic embryogenesis is the key to in vitro regeneration [3][4][5]. Somatic embryogenesis is the most effective technique for the large-scale propagation of plants and the long-term conservation of different embryogenic cell lines.…”

Section: Introductionmentioning

confidence: 99%

“…The low efficiency of somatic embryo induction is among the major problems hindering efficient regeneration and limiting practical applications. Therefore, high-quality somatic embryo production is one of the most important factors in somatic embryogenesis protocols for commercial propagation and breeding programs [6,7].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Amino Acids on Callus Proliferation and Somatic Embryogenesis in Litchi chinensis cv. ‘Feizixiao’

Wang,

Liu,

Gao

et al. 2023

Horticulturae

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Amino acids (AAs) are important regulators of morphogenesis during somatic embryogenesis. This study investigated the roles of AAs in callus proliferation and embryo induction in Litchi chinensis Sonn. cv. ‘Feizixiao’ to improve the current in vitro regeneration protocols toward supporting efficient commercial litchi propagation and breeding programs. During callus proliferation, the total AAs ranged from 4627.9 μg·gFW−1 on day 3 to 7827.8 μg·gFW−1 on day 21, with an average content of 5994.0 μg·gFW−1. The total AA content continuously decreased to 3390.1 μg·gFW−1 on day 10 and slightly increased between days 10 and 20 during embryo induction, with an average content of 4849.7 μg·gFW−1. At both stages, γ-aminobutyric acid, arginine, alanine, and glutamine accounted for more than 10% of the total AAs, and methionine, tryptophan, glycine, and cystine contents were lower than 1%, except for the tyrosine content, which was also lower than 1% at the embryo induction stage. The callus proliferated on medium supplemented with a low concentration of AMO1 solution, a mixture of 21 amino acids prepared according to the average proportion of each amino acid during callus proliferation. The calli were small, yellow, and uniform, with deep Ehrlich hematoxylin staining. When cultured on medium containing a high concentration of AMO1, the callus gradually turned brown, with more dark yellow proembryos, and cell lysis and apoptosis occurred. The combination of adding 4× AMO1 solution to the proliferation medium and adding 1× AMO2 solution, which was mixed with 21 amino acids according to their average proportion during embryo induction, to the embryo induction medium had the best regeneration efficiency, with about 396 embryos and 88 regenerated plantlets per gram of callus. The results provide a basis for the rational combined application of AAs during the in vitro regeneration of litchi to achieve greater somatic embryogenesis efficiency.

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Section: Callus Induction and Culturementioning

confidence: 99%

Section: Msmentioning

confidence: 99%