In vitro propagation of eight species or subspecies of Turbinicarpus (Cactaceae)

Dávila-Figueroa, Carlos Antonio; Rosa-Carrillo, Ma. de Lourdes de la; Pérez-Molphe-Balch, Eugenio

doi:10.1079/ivp2005668

Cited by 16 publications

(12 citation statements)

References 10 publications

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“…This is a frequent result reported for many other cacti rooting in vitro on auxin-free media, e.g., Coryphantha elephantidens (Wakhlu and Bahu, 2000); Turbinicarpus spp. (Dávila-Figueroa et al, 2005); Notocactus magnificus (de Medeiros et al, 2006); and in particular those species that root freely in vivo such as Opuntia amyclaea (Escobar et al, 1986); Agave parrasana (Santacruz-Ruvalcaba et al, 1999);…”

Section: Discussionmentioning

confidence: 99%

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Micropropagation and Reintroduction of the Endemic Mammillaria mathildae (Cactaceae) to Its Natural Habitat

García-Rubio¹,

Malda-Barrera²

2010

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A rapid shoot multiplication protocol was established for the endangered cactus Mammillaria mathildae to reintroduce it to its natural habitat. In vitro-germinated seedlings were used as the source of explants. Three explant sources (apical, lateral, and basal excised from in vitro-germinated seedlings) were tested. Shoot multiplication was induced in Murashige and Skoog (MS) medium supplemented with different 6-benzylaminopurine/indole-3-acetic acid (BA/IAA) combinations (0, 22.19, 44.39 and 0, 1.43, 2.85, 5.71, respectively). Explants developed abundant callus in the presence of any BA/IAA concentration, whereas hormone-free media produced 0.59 ± 0.11 new shoots (with a 41% callus development) from basal explants. Apical and lateral explants produced 1.14 ± 0.07 and 4.09 ± 0.13 new shoots, respectively, without callus generation. Plantlets originating from lateral explants developed a vigorous rooting system after 2 months growing on MS medium supplemented with 30 g·L−1 sucrose. Under greenhouse conditions, 98% of micropropagated M. mathildae survived. Plantlets were reintroduced in an experimental plot near to Juriquilla's wild population of M. mathildae; over 52% of the outplanted M. mathildae lot declined after 5 months. Water availability was associated with the decline of outplanted populations during the first month (43%).

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Section: Discussionmentioning

confidence: 99%

“…(Pérez-Molphe-Balch and Dávila-Figueroa, 2002); Ariocarpus kotschoubeyanus (Moebius-Goldammer et al, 2003); Turbinicarpus spp. (Dávila-Figueroa et al, 2005); Notocactus magnificus (de Medeiros et al, 2006); 10 different Mammillaria spp. (Ramirez-Malagon et al, 2007); and so on.…”

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confidence: 99%

Micropropagation and Reintroduction of the Endemic Mammillaria mathildae (Cactaceae) to Its Natural Habitat

García-Rubio¹,

Malda-Barrera²

2010

horts

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“…Concentrations of growth regulators in the growth medium used for cactus species range from 0, as in the case of in vitro culture of Mammillaria gracilis Pfeiff. (Balen et al, 2004), to low, intermediate or high levels of cytokinins, such as BA (4.4, 6.7, 8.8, 13.3 and 44.4 µM), employed alone in several species of Mammillaria (Mammillaria longimamma, Mammillaria stylothele), Turbinicarpus and Escobaria vivipara, or in combinations with an auxin like NAA (2.7-10.7 µM) or IAA (2.9-5.7 µM) with Notocactus (Mata Rosas et al, 2001;Dávila-Figueroa et al, 2005;Medeiros et al, 2006;Quiala et al, 2009). Growth media have been supplemented with Kin at 0, 4.6, 13.9, 22.3, 27.9 and 46.5 µM in the absence or presence of an auxin (IAA or NAA), and zeatin has been added at 4.6 and 22.3 µM for in vitro multiplication of species of the genera Pediocactus, Sclerocactus and Toumeya (Clayton et al, 1990).…”

Section: Growth Regulators For Cacti Tissue Culturementioning

confidence: 99%

Tissue culture of ornamental cacti

Pérez-Molphe-Balch

Santos-Díaz

Ramírez-Malagón

et al. 2015

Sci. agric. (Piracicaba, Braz.)

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Cacti species are plants that are well adapted to growing in arid and semiarid regions where the main problem is water availability. Cacti have developed a series of adaptations to cope with water scarcity, such as reduced leaf surface via morphological modifications including spines, cereous cuticles, extended root systems and stem tissue modifications to increase water storage, and crassulacean acid metabolism to reduce transpiration and water loss. Furthermore, seeds of these plants very often exhibit dormancy, a phenomenon that helps to prevent germination when the availability of water is reduced. In general, cactus species exhibit a low growth rate that makes their rapid propagation difficult. Cacti are much appreciated as ornamental plants due to their great variety and diversity of forms and their beautiful short-life flowers; however, due to difficulties in propagating them rapidly to meet market demand, they are very often over-collected in their natural habitats, which leads to numerous species being threatened, endangered or becoming extinct. Therefore, plant tissue culture techniques may facilitate their propagation over a shorter time period than conventional techniques used for commercial purposes; or may help to recover populations of endangered or threatened species for their re-introduction in the wild; or may also be of value to the preservation and conservation of the genetic resources of this important family. Herein we present the state-of-the-art of tissue culture techniques used for ornamental cacti and selected suggestions for solving a number of the problems faced by members of the Cactaceae family.

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“…It could be considered fast based on data for germination of eight cacti species of the genus Turbinicarpus, whose onset of in vitro germination was on the fourteenth day (DÁVILA-FIGUEROA et al, 2005) and on similar results observed for Pilosocereus robinii (QUIALA et al 2009), whereas Arrojadoa spp (cactus foxtail) germination began on the eighth day after sowing (DIAS et al, 2008).…”

Section: Resultsmentioning

confidence: 84%

Germinação in vitro e aclimatização de Hamatocactus setispinus

Xavier¹,

Jasmim²

2015

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Seed propagation preserves the population genetic variability and helps selecting desirable features. This study evaluated the in vitro germination of Hamatocactus setispinus in six different culture media, 1-MS basal medium full strength; 2-halfstrength MS basal medium; 3-1.0 g L -1 of Peter's CalMag ® 15-05-15 formulation; 4-0.5 g L -1 of Peter's CalMag ® 15-05-15 formulation; 5-MS basal medium supplemented with 10% coconut water and; 6-water and agar, with and without activated charcoal, and the speed of germination index, the mean germination time and the germination rate, root length, shoot length and the number of roots were evaluate. The seedlings with superior development obtained from in vitro germination were acclimatized in two substrates: Biomix ® Floreira; Biomix ® Floreira + sand. Seedling survival, shoot length, shoot diameter, root length, root number, shoot fresh matter weight, root fresh matter weight, shoot dry matter weight and root dry matter weight were evaluated. Peter's 1.0 g L -1 medium without activated charcoal led to the best results for root length (11.36 mm) and root number (3.84). There was 100% of seedling survival. Acclimatization substrates did not differ among themselves and, therefore, they did not affect seedling growth. Keywords: Hamatocactus setispinus, activated charcoal, culture media, cacti. RESUMOGerminação in vitro e aclimatização de Hamatocactus setispinus A propagação de sementes preserva a variabilidade genética da população e ajuda a selecionar características desejáveis. Objetivou-se estudar a germinação in vitro de Hamatocactus setispinus em seis diferentes meios de cultura, 1-meio de cultivo MS; 2-MS ½ força de sais minerais; 3-1 g L -1 da formulação 15-05-15 Peter's ® CalMag; 4-0,5 g L -1 da formulação 15-05-15 Peter's ® CalMag; 5-MS com adição de 10% de água de coco e; 6-água e ágar, com e sem carvão ativado, avaliando o índice de velocidade de germinação, o tempo médio de germinação e a taxa de germinação, comprimento da raiz, comprimento da parte aérea e o número de raízes. As plântulas com desenvolvimento superior obtidas da germinação in vitro foram aclimatizadas em dois substratos: Biomix ® Floreira; Biomix ® Floreira + areia. Foram avaliados: sobrevivência de plântulas, comprimento da parte aérea, diâmetro da parte aérea, comprimento da raiz, número de raízes, matéria fresca da parte aérea, matéria fresca da raiz, matéria seca da parte aérea e matéria seca da raiz. O meio Peter's 1,0 g L -1 sem carvão ativado levou a melhores resultados no comprimento de raiz (11,36 mm) e número de raízes (3,84). Houve 100% de sobrevivência das mudas. Os substratos de aclimatação não diferiram entre si e, por isso não afetaram o crescimento das mudas. Palavras-chave: Hamatocactus setispinus, carvão ativado, meio de cultura, cacto.(1) Received in 15/08/2013 and approved in 10/06/2015.

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In vitro propagation of eight species or subspecies of Turbinicarpus (Cactaceae)

Cited by 16 publications

References 10 publications

Micropropagation and Reintroduction of the Endemic Mammillaria mathildae (Cactaceae) to Its Natural Habitat

Micropropagation and Reintroduction of the Endemic Mammillaria mathildae (Cactaceae) to Its Natural Habitat

Tissue culture of ornamental cacti

Germinação in vitro e aclimatização de Hamatocactus setispinus

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