In vitro propagation of Dendrobium aphyllum (Orchidaceae)—seed germination to flowering

Medicinal orchids and their uses: Tissue culture a potential alternative for conservation

“…Different protocols have been developed for the large-scale propagation of a number of orchid species and hybrids through in vitro culture of various parts including shoot tips, flower stalk, nodes, buds, stems, root tips and rhizome segments (Vij, 1993;Nayak , 1998;Kanjilal et al, 1999;Chang and Chang, 2000b;Chen et al, 2003b;Chugh et al, 2009;Pant and Shrestha, 2011;Deb and Pongener, 2012;Paudel and Pant, 2012). Mass propagation of medicinal orchids using in vitro culture technique has been reported by some workers (Sharma and Chandel, 1996;Liu and Zhang, 1998); Nalawade et al, 2003;Shiau et al, 2005;Basker and Bai 2006;Sharma et al, 2007;Pant et al, 2008;Hossain et al, 2009Hossain et al, , 2012Kaur andBhutani, 2009, 2010;Nongdam and Chongtham, 2011;Pant and Thapa, 2012;Pradhan et al, 2013). There are very few reports on reintroduction of in vitro propagated species of medicinal orchids to natural habitat (Stewart and Kane, 2006;Aggarwal and Zettler, 2010;Lesar, 2012.…”

Establishment of protocorm suspension cultures of Dendrobium candidum for the production of bioactive compounds

“…A large number of organic supplements like peptone, banana homogenate, coconut water and potato extract are commonly added in the orchid culture media and their beneficial effects have been reported (Paek and Murthy, 2002;Hossain et al, 2013). In the present study, we have tested the effect of banana homogenate (1-9%, v/v), coconut water (1-9%, v/v) and potato extract (1-9%, v/v) on protocorm biomass accumulation and production of bioactive compounds.…”

Ex Vitro Seedling Development from In Vitro Rhizome-Like Bodies in Eulophia promensis Lindl.: A New Technique for Orchid Propagation

“…The cause of maximum germination of seeds in P 723 medium compared to MS medium could be due to the fact that P 723 medium was enriched with peptone. Various complex substitutes including peptone with basal medium have been reported to improve seed germination and subsequent protocorm development in a number of orchids [6,8,13,22,23]. Peptone contains amino acids, amides, minor elements, and vitamins responsible for Mean values within a column followed by the same letter were not significantly different at = 0.05. enhancement of seed germination and growth of protocorms [24].…”

“…Germination of orchid seeds followed a peculiar metamorphogenetic pathway; that is, undifferentiated embryos swelled up by absorbing water and nutrients from the media and developed a compact mass of parenchymatous cells called spherule which gradually develop protocorm, an intermediate structure between seed and seedling [33]. At the initial stage of protocorm development, an appendicle, looking like a closed ridge, appeared at the upper part of the protocorms which leads to shoot formation while basal part escorts root development [6,8,13,14]. In E. promensis a different type of morphogenesis was observed.…”