In vitro propagation of Dendrobium aphyllum (Orchidaceae)—seed germination to flowering

Hossain, M. M.; Sharma, Madhu; Pathak, Pankaj

doi:10.1007/s13562-012-0124-3

Cited by 34 publications

(17 citation statements)

References 51 publications

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“…Different protocols have been developed for the large-scale propagation of a number of orchid species and hybrids through in vitro culture of various parts including shoot tips, flower stalk, nodes, buds, stems, root tips and rhizome segments (Vij, 1993;Nayak , 1998;Kanjilal et al, 1999;Chang and Chang, 2000b;Chen et al, 2003b;Chugh et al, 2009;Pant and Shrestha, 2011;Deb and Pongener, 2012;Paudel and Pant, 2012). Mass propagation of medicinal orchids using in vitro culture technique has been reported by some workers (Sharma and Chandel, 1996;Liu and Zhang, 1998); Nalawade et al, 2003;Shiau et al, 2005;Basker and Bai 2006;Sharma et al, 2007;Pant et al, 2008;Hossain et al, 2009Hossain et al, , 2012Kaur andBhutani, 2009, 2010;Nongdam and Chongtham, 2011;Pant and Thapa, 2012;Pradhan et al, 2013). There are very few reports on reintroduction of in vitro propagated species of medicinal orchids to natural habitat (Stewart and Kane, 2006;Aggarwal and Zettler, 2010;Lesar, 2012.…”

Section: Propagation Of Orchids Using Different Explantsmentioning

confidence: 99%

Medicinal orchids and their uses: Tissue culture a potential alternative for conservation

Pant¹

2013

Afr. J. Plant Sci.

164

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Section: Propagation Of Orchids Using Different Explantsmentioning

confidence: 99%

Medicinal orchids and their uses: Tissue culture a potential alternative for conservation

Pant¹

2013

Afr. J. Plant Sci.

164

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“…A large number of organic supplements like peptone, banana homogenate, coconut water and potato extract are commonly added in the orchid culture media and their beneficial effects have been reported (Paek and Murthy, 2002;Hossain et al, 2013). In the present study, we have tested the effect of banana homogenate (1-9%, v/v), coconut water (1-9%, v/v) and potato extract (1-9%, v/v) on protocorm biomass accumulation and production of bioactive compounds.…”

Section: Resultsmentioning

confidence: 99%

Establishment of protocorm suspension cultures of Dendrobium candidum for the production of bioactive compounds

Cui

Murthy

Moh³

et al. 2015

Hortic. Environ. Biotechnol.

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Dendrobium candidum is an important medicinal plant rich in polysaccharides, phenols, flavonoids and its bioactive compounds are known for various pharmacological activities. Natural populations of D. candidum are depleting due to overexploitation and increasing demand for medicinal and health products. An efficient protocol for protocorm suspension cultures of Dendrobium candidum in shake flasks was developed and the effect of auxins, basal media, nitrogen concentration, sucrose concentration, organic supplements like banana homogenate, coconut water and potato extract were investigated for the production of biomass and bioactive compounds. For the production of polysaccharides, polyphenolics, and flavonoids, the optimized culture conditions were found to be MS basal medium supplemented with 0.5 mg·L -1 NAA, 5:25 mM NH4:NO3 as nitrogen source, 2.5% (w/v) sucrose, and 1% (v/v) banana homogenate. Results of the current study could be used for scale-up process.

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“…The cause of maximum germination of seeds in P 723 medium compared to MS medium could be due to the fact that P 723 medium was enriched with peptone. Various complex substitutes including peptone with basal medium have been reported to improve seed germination and subsequent protocorm development in a number of orchids [6,8,13,22,23]. Peptone contains amino acids, amides, minor elements, and vitamins responsible for Mean values within a column followed by the same letter were not significantly different at = 0.05. enhancement of seed germination and growth of protocorms [24].…”

Section: Resultsmentioning

confidence: 99%

Ex Vitro Seedling Development from In Vitro Rhizome-Like Bodies in Eulophia promensis Lindl.: A New Technique for Orchid Propagation

Hossain

2015

Journal of Botany

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This communication describes in vitro seed germination, embryo differentiation, and ex vitro seedling production from in vitro rhizome-like bodies of a terrestrial orchid, Eulophia promensis Lindl. Effects of two nutrient media, namely, Murashige and Skoog (MS) and Phytotechnology Orchid Seed Sowing medium (P723) supplemented with 6-benzylaminopurine (BAP; 0.5–1.0 mgL−1) and/or α-naphthalene acetic acid (NAA; 0.5–1.0 mgL−1) and activated charcoal (2.0 gL−1), were studied on seed germination and subsequent development of embryos. Maximum seed germination (100%) was recorded in P723 medium fortified with 1.0 mgL−1 BAP + 2.0 gL−1 activated charcoal. The different developmental stages of protocorm morphogenesis were traced out. In subsequent subcultures, the protocorms proliferated profusely and developed rhizome-like bodies (RLBs) with numerous hair-like structures. These RLBs were transferred to pots containing potting mixture composed of humus + coir dust + saw dust (1 : 1 : 1) where ∼80% of RLBs survived and produced 1–3 seedlings per RLB. This is the first time report for in vitro germination of seeds and ex vitro seedling production from in vitro raised RLBs in Eulophia promensis. This is a time saving and cost effective protocol that could be extended to other economically important, rare, and endangered orchids for propagation and conservation.

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In vitro propagation of Dendrobium aphyllum (Orchidaceae)—seed germination to flowering

Cited by 34 publications

References 51 publications

Medicinal orchids and their uses: Tissue culture a potential alternative for conservation

Medicinal orchids and their uses: Tissue culture a potential alternative for conservation

Establishment of protocorm suspension cultures of Dendrobium candidum for the production of bioactive compounds

Ex Vitro Seedling Development from In Vitro Rhizome-Like Bodies in Eulophia promensis Lindl.: A New Technique for Orchid Propagation

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