Medicinal orchids and their uses: Tissue culture a potential alternative for conservation

Pant, Bijaya

doi:10.5897/ajps2013.1031

Cited by 177 publications

(116 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Por la intensa explotación debido a que tiene gran valor ornamental y medicinal, en algunos países los tubérculos secos son comúnmente utilizados como un tónico, pero rara vez se ha propagado sexualmente o mejorado debido a la dificultad de propagación de sus semillas (Hirano et al 2009, Pant y Shrestha 2011, Pant 2013), ya que la misma presenta muy baja germinación aún en condiciones in vitro (Pant et al 2011).…”

Section: Introductionunclassified

Tamaño y viabilidad de semillas: implicancias en la evolución y conservación de Phaius tankervilleae (Orchidaceae)

Duarte¹,

Mangeón²,

Küppers³

et al. 2017

Caldasia

View full text Add to dashboard Cite

Tamaño y viabilidad de semillas: implicancias en la evolución y conservación de Phaius tankervilleae (Orchidaceae)Seed size and viability: implications on the evolution and conservation of Phaius tankervilleae (Orchidaceae) Argentina. vane.mangeon@hotmail.com, guillo_lq@hotmail.com, procha910@gmail.com, fernandoniella@gmail.com EvElyn RESUMENPhaius tankervilleae es una orquídea terrestre altamente apreciada como ornamental y medicinal, razón por la cual se encuentra en peligro de extinción en algunos países como Australia y Japón. Generar información sobre las dimensiones y el vigor de la semilla es importante para entender aspectos de la evolución de la especie y encontrar métodos de conservación. Este estudio tuvo como finalidad poner a prueba la hipótesis, de que el tamaño y viabilidad de las semillas de P. tankervilleae están positivamente relacionados con el tamaño del fruto. Para realizar el estudio se cosecharon frutos de diferentes tamaños, de los cuales se tomaron muestras de 2,5 mg de semillas por frutos. Se realizó la caracterización morfológica de las semillas mediante observaciones en microscopio. Las semillas de cada tamaño de fruto fueron sometidas a un test de Tetrazolio para determinar viabilidad y se les tomaron mediciones del largo, ancho y volumen tanto de la testa como del embrión. Las semillas con embrión son fusiformes y las vanas son filiformes, el embrión es relativamente pequeño y las semillas de fruto mediano son las que presentan menor espacio de aire. Los resultados confirman la hipótesis planteada, cuanto más pequeño es el fruto menor es el tamaño y la cantidad de semillas viables. Por consiguiente, si se desea tener mayor cantidad de semillas de buena calidad es recomendable cosechar frutos de mayor tamaño.Palabras clave. Embrión, orquídea, tetrazolio, testa, volumen. ABSTRACTPhaius tankervilleae is a terrestrial orchid highly prized as ornamental and medicinal, which is why it is in danger of extinction in some countries such as Australia and Japan. Generating information on seed size and vigor is important in understanding aspects of the evolution of the species and finding conservation methods. This study aimed to test the hypothesis that the size and viability of P. tankervilleae seeds are positively related to fruit size. In order to carry out the study, fruits of different sizes were harvested, from which samples of 2.5 mg of seeds per fruit were taken. Morphological characterization of the seeds was carried out by means of microscope observations. A Tetrazolium test was carried out to determine the viability of seeds and measurements were taken of the length, width, and volume of both the testa and the embryo. Seeds with embryos are fusiform and the empty one are filiform, the embryo consERvación

show abstract

Section: Introductionunclassified

Tamaño y viabilidad de semillas: implicancias en la evolución y conservación de Phaius tankervilleae (Orchidaceae)

Duarte¹,

Mangeón²,

Küppers³

et al. 2017

Caldasia

View full text Add to dashboard Cite

show abstract

“…It is used as tonic and in general debility. [11][12][13][14][15][16][17][18] It has been found that in spite of its ethnomedicinal value, little work has been done on this plant. In the current investigation carried out, pharmacognostic evaluation and screening of different extracts of C. acuminatum pseudobulbs have been used against various types of bacteria in order to screen new sources of antimicrobial agents.…”

Section: Pharmacogostic Evaluationmentioning

confidence: 99%

“…[14][15][16][17][18][19][20][21][22][23][24]25 whereas in another study Piperitone, Citronellal, Eugenol, Limonene, 1,8-Cineole, p-Cymene, o-Methylbatatasin, has been reported by thin layer chromatography. [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] whereas in another study metal content and volatile constituents in Microstylis wallichii (C. acuminatum) which were analyzed by Atomic Absorption Spectrophotometer and GC and GC-MS. Not much work has been done on the phytoconstituents of this plant. Sharma, P and coworkers 27 studied The antimicrobial activity of pseudobulbs of Malaxis acuminata using the Butanol extract against some gram (+)ve, Gram (-)ve bacteria and fungi by agar cup diffusion method against Escherichia coli, Klebsiella aerogenes, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus and fungus, Candida albicans.…”

mentioning

confidence: 99%

Pharmacognostic Evaluation & Antimicrobial Activity of Endangered Ethnomedicinal Plant Crepidium acuminatum (D. Don) Szlach

Arora¹,

Kaur²,

Kahlon³

et al. 2017

View full text Add to dashboard Cite

Background:Crepidium acuminatum (D. Don) Szlach (family Orchidaceae) is an ethnomedicinal plant. It is used in breathing disorders, burning sensation, Cough, decrease in bone tissue, blood disorders, tuberculosis, as refrigerant, aphrodisiac, in insect bites, rheumatism, as tonic and in general debility. It is vital component of Ayurvedic formulation "Astavarga" with trade name "Jeevak means vitality of life. Despite the common utilization of this plant, no conclusive study has been reported so far regarding the pharmacognostic evaluation and antimicrobial activity. Aim: The present study was carried to evaluate pharmacognostic evaluation and the potential of C. acuminatum as antimicrobial. Materials and Methods: Organoleptic, histochemical, microscopic, physicochemical, extractive yield were studied to standardise pharmacognostic characters and well diffusion method were carried out for antimicrobial activity. Five extracts viz. Hexane, Chloroform, Ethanol, Ethyl acetate and aqueous were evaluated against 4 bacterial strains viz. E. coli (MTCC 40), S. aureus (MTCC 87), P. aeruginosa (MTCC 424), B. subtilis (MTCC 121). Results: The diagnostic characters were evaluated and documented. All the extracts showed good antimicrobial activity. Conclusion: Obtained standards will provide referential information for correct identification, purity, standardization and preparation of monograph. The work confirms that the studied plant has potent antimicrobial activity and has potential for antimicrobial drug. These results may constitute a basis for promising future applied research that could investigate the use of this plant as antimicrobial drug.

show abstract

“…Nepal and other Asian countries, such as Indonesia, Malaysia, Taiwan, Singapore, Vietnam, Shri Lanka, Thailand, Myanmar, etc., have been using orchids in traditional medicine [10].…”

mentioning

confidence: 99%

Ethnobotany, Pharmacology and Chemistry of Medicinal Orchids from Veracruz

Asseleih¹,

García²,

Cruz³

2015

JAST-A

View full text Add to dashboard Cite

Medicinal orchids and their uses: Tissue culture a potential alternative for conservation

Cited by 177 publications

References 60 publications

Tamaño y viabilidad de semillas: implicancias en la evolución y conservación de Phaius tankervilleae (Orchidaceae)

Tamaño y viabilidad de semillas: implicancias en la evolución y conservación de Phaius tankervilleae (Orchidaceae)

Pharmacognostic Evaluation & Antimicrobial Activity of Endangered Ethnomedicinal Plant Crepidium acuminatum (D. Don) Szlach

Ethnobotany, Pharmacology and Chemistry of Medicinal Orchids from Veracruz

Contact Info

Product

Resources

About