In vitro propagation of Ceropegia thwaitesii Hook- an endemic species of Western Ghats of Tamil Nadu, India

Muthukrishnan, S.; Benjamin, JH Franklin; Muthukumar, M.; Sherif, N. Ahamed; Kumar, T. Senthil; Rao, M. S.

doi:10.5897/ajb11.3401

Cited by 9 publications

(5 citation statements)

References 32 publications

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“…Fresh plant parts (stem and leaves) of mother/ in vivo (IVP) as well as in vitro raised plants (IRP) of C. thwaitesii were thoroughly washed under running tap water and blotted with tissue paper. For using in vitro plant material, the plant culture has been adapted as described earlier [16]. One gram leaves and stems of in vitro and in vivo fresh material was homogenized in 25 mL of respective organic solvent (methanol, ethanol, petroleum ether, acetone, chloroform and water) and extractions were carried on orbital shaker (REMI, India) with constant stirring at 120 rpm for overnight.…”

Section: Methodsmentioning

confidence: 99%

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Phytochemical analysis, antioxidant and antimicrobial activity of wild and in vitro derived plants of Ceropegia thwaitesii Hook – An endemic species from Western Ghats, India

Muthukrishnan

Kumar

Gangaprasad

et al. 2018

Journal of Genetic Engineering and Biotechnology

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Ceropegia thwaitesii Hook (Asclepiadaceae), an endemic plant species, due to habitat destruction and over exploitation has a very restricted distribution in the Western Ghats of Tamil Nadu, India. The present wrok aimed to determine the chemical composition, the total phenolic (TPC), flavonoid (TFC) and tannin content (TEC), and to assess the antioxidant properties of various extracts of in vivo plants (IVP) and in vitro regenerated plants (IRP) of C. thwaitesii. Some phenolic compounds like gallic acid, cathechol, vanillin and salicylic acid were identified and quantified by HPLC. All the extracts possessed relevant radical scavenging activity on DPPH, Superoxide radical scavenging activity, and Nitric oxide radicals as well as total antioxidant ability. DPPH assay of in vitro methanol stems extracts and ethanol leaves extracts revealed the best antioxidant properties with important IC50 values of 0.248 ± 0.45 µg/mL and 0.397 ± 0.67 µg/mL, respectively, whereas in vivo chloroform stems extracts showed a lower antioxidant activity (IC50 of 10.99 ± 0.24 µg/mL). The IRP methanol extracts of stem and leaves had good inhibitory activity against all tested microorganisms in a dose-dependent manner. These results suggested that in vitro raised plants of C. thwaitesii are an excellent source of antioxidant compounds to be exploited on an industrial level as food additive.

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Section: Methodsmentioning

confidence: 99%

“…It inhabits bare slopes of Pambar shola, and is highly vulnerable due to habitat destruction and over exploitation [15]. Ex vivo protocol for its multiplication has been developed and reported earlier by the author [16], [17].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical analysis, antioxidant and antimicrobial activity of wild and in vitro derived plants of Ceropegia thwaitesii Hook – An endemic species from Western Ghats, India

Muthukrishnan

Kumar

Gangaprasad

et al. 2018

Journal of Genetic Engineering and Biotechnology

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“…Some other plant species also showed dependence on cytokinins viz; C. elegans [25], C. spiralis [26], Holostemma ada-kodien [27] and Hemidusmus indicus [28]. The average performance of TDZ was demonstrated in the present study as well as in C. thwaiitesii [29]. Enhanced shoot induction was obtained by using cytokinin BAP along with Auxin in some species like H. indicus [28] and Holostema annulare [30] while In vitro flowering in C. anjanerica was observed on MS ?…”

Section: Resultsmentioning

confidence: 50%

In Vitro Propagation of Ceropegia anjanerica Malpure et al.: A Rare Endemic Plant from Maharashtra

Nalawade

Desai

Gaikwad

et al. 2014

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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An in vitro micropropagation system has been developed for the Ceropegia anjanerica Malpure et al., a rare endemic plant species having ornamental as well as medicinal potential but a limited reproductive capacity. Axillary bud explants were cultured on Murashige and Skoog medium supplemented with 6-Benzylaminopurine, Thidiazuron and 6-Furfurylaminopurine along with combination of Indole-3-Butyric Acid. Although all concentrations showed multiple shoot formation, the best results (8.0 ± 0.5) were achieved with MS ? 6-Benzylaminopurine (8.87 lM) ? 6-Furfurylaminopurine (18.58 lM). Half strength Murashige and Skoog medium fortified with Indole-3-Acetic Acid (8.56 lM) showed good rooting response (4.0 ± 0.7). Well grown plantlets hardened, acclimatized and established in greenhouse exhibited 78 % survival.

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“…Best rooting was also observed in many Asclepideace ae members such as Gymnema sylvestre (Komalavalli & Rao 2000), Ceropegia candelabrum (Beena et al 2003), C. thwaitesii (Muthukrishnan et al 2012), C. bulbosa and C. juncea (Sub baiyan et al 2015). MS medium fortified either with indi vidual different concentrations of auxins or in combination with cytokinins could induce callus, somatic embryogenesis, proliferations of shoot and root, tuber formations and en hance the secondary metabolites productions as reported by many researchers (Ghanti et al 2004, Desai et al 2014.…”

Section: Discussionmentioning

confidence: 99%

Plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa Roxb., an endangered medicinal plant

Subbaiyan¹,

Thangapandian²

2017

Bot Pac

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An efficient protocol of callus induction, shoot multiplication and plant rege ne ra tion from cotyledon and leaf explants of Ceropegia bulbosa has been elaborated. The highest percentage (90.66) of callus induction was observed when cotyle do nary explants cultured on MS medium having 0.5 mg/L 2,4D in combination with 0.05 mg/L Kn followed by 86.25 % callus induction from leaf explant at 0.5 mg/l BAP in combination with 0.05 mg/l Kn. Shoot multiplication was ob served, cotyledon derived callus cultured on 1.0 mg/L BAP in combination with 0.10 mg/L NAA could induce number of shoots (4.71) and their lengths (6.13 cm). Cotyledonary callus derived microshoots gave the highest rooting percen tage (92.26 %) root numbers (13.14) and their lengths (8.87 cm) were observed on halfstrength MS medium fortified with 0.4 mg/L IBA. In vitro derived plant lets were hardened in the greenhouse and successfully established into the soil. Переведено редакцией.

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In vitro propagation of Ceropegia thwaitesii Hook- an endemic species of Western Ghats of Tamil Nadu, India

Cited by 9 publications

References 32 publications

Phytochemical analysis, antioxidant and antimicrobial activity of wild and in vitro derived plants of Ceropegia thwaitesii Hook – An endemic species from Western Ghats, India

Phytochemical analysis, antioxidant and antimicrobial activity of wild and in vitro derived plants of Ceropegia thwaitesii Hook – An endemic species from Western Ghats, India

In Vitro Propagation of Ceropegia anjanerica Malpure et al.: A Rare Endemic Plant from Maharashtra

Plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa Roxb., an endangered medicinal plant

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