In vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesis

Osorio, Catalina Restrepo; Velásquez, Felipe Andrés Gómez; Correal, Alejandro Gil; Bonilla, Javier Mauricio Torres; Trujillo, Aura Inés Urrea

doi:10.15446/acag.v67n1.61474

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…cv. Hass ( Osorio et al., 2018 ), have achieved plantlet elongation by lowering the concentration of basic medium or the phytohormone concentration. In the present study, selecting the appropriate medium and reducing the nitrogen and phosphorus contents were conducive to adventitious shoot elongation at the stage of obvious stem differentiation ( Table 1 ; Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

An efficient in vitro organogenesis protocol for the endangered relic tree species Bretschneidera sinensis and genetic fidelity assessment using DNA markers

Yan,

Zheng,

et al. 2024

Front. Plant Sci.

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Bretschneidera sinensis is a monotypic species of rare and tertiary relic trees mainly distributed in China. B. sinensis is a potentially valuable horticultural plant, which has significant ornamental and research value, and is a crucial tool for the study of phylogeography. The artificial cultivation of B. sinensis is of great scientific value and practical significance. In this study, we developed a direct organogenesis process of B. sinensis using mature zygotic embryos as initial materials. The highest sterile germination induction (54.5%) from the mature zygotic embryo was obtained in a Murashige and Skoog (MS) medium with 2.0 mg·L−1 6-benzylaminopurine (6-BA) and 0.2 mg·L−1 α-naphthaleneacetic acid (NAA). The highest percentage of shoot regeneration (90.37%) was attained using 1.0 mg·L−1 6-BA and 0.01 mg·L−1 NAA in the MS medium. The Woody Plant Medium (WPM) had the greatest adventitious shoot elongation rate of 93.33%. The most optimized rooting rate was 88.89% in a half-strength MS medium containing 2.0 mg·L−1 indole-3-butyric acid (IBA) and 1.0 mg·L−1 NAA. The genetic fidelity of in vitro regenerated plantlets was assessed using inter-simple sequence repeats and random amplified polymorphic DNA molecular markers, confirming the genetic uniformity and stability of regenerated B. sinensis plantlets. Our research presents an effective in vitro propagation system for B. sinensis, laying the groundwork for its germplasm conservation and large-scale production while maintaining high genetic integrity.

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Section: Discussionmentioning

confidence: 99%

An efficient in vitro organogenesis protocol for the endangered relic tree species Bretschneidera sinensis and genetic fidelity assessment using DNA markers

Yan,

Zheng,

et al. 2024

Front. Plant Sci.

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Regeneración de explantes de hojas de cinco genotipos de frambuesa

Rosas-Rojas,

Ochoa-Alejo,

Rocha-Granados

2023

Remexca

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La eficiencia en la regeneración de frambuesa, a partir de esplantes de hojas, se ve limitada debido a diversos factores, entre que los que destacan la edad del explante y el genotipo. El objetivo de esta investigación fue determinar el efecto de los reguladores de crecimiento sobre la oxidación y la regeneración in vitro a partir de explantes de hojas de cinco genotipos de frambuesa, el año del estudio fue en 2021. Se probaron dosis y combinaciones de auxinas y citocininas para inducir organogénesis directa en explantes foliares de los genotipos de frambuesa; ‘C-6’, ‘Joan J.’, ‘A-1’, ‘UM-702’ y ‘Heritage’. Los resultados mostraron que el regulador bencilaminopurina (BAP) disminuyó la oxidación en los genotipos ‘C-6’, ‘Joan J.’, ‘A-1’ y ‘Heritage’ en 36, 48, 60 y 68% respectivamente, los que se suplementaron con cinetina tuvieron una reducción de la oxidación en el genotipo ‘C-6’ (56%), cuando se adicionó tidiazuron (TDZ) la oxidación disminuyó en los genotipos evaluados en 72, 64, 72, 84 y 68% respectivamente. La mayor regeneración (número de brotes/explate) fue con BAP (0.5 mg L-1) y TDZ (0.2 mg L-1) + ácidoindolbutírico (AIB) (0.1 mg L-1) para el genotipo ‘C-6’, y TDZ (0.2 mg L-1) + AIB (0.1 mg L-1) para ‘Joan J.’ y ‘Heritage’. En ‘A-1’y ‘UMC-702’ se sugiere el uso de TDZ (0.2 mg L-1) solo. Se concluye que el uso de reguladores de crecimiento, solos o combinanados, disminuyen la oxidación en los explantes de hojas, y aumentan la sobrevivencia y regeneración de brotes en todos los genotipos evaluados.

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Application of Etiolation of Grape Explants in Vitro Culture

Zelenyanska,

Gogulinska,

Artiukh

et al. 2024

Naukovì dopovìdì Nacìonalʹnogo unìversitetu bìoresursìv ì priro

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Micropropagation of grapes is used to obtain original and certified planting material for planting mother plants. The decrease in the efficiency of microclonal reproduction is associated with difficulties at the stages of introducing explants into in vitro culture and rooting. The method of etiolation is simple to use and allows you to prevent phenolic oxidation of explants, has a positive effect on increasing the length of shoots and the number of roots. The purpose of the research was to determine the effectiveness of etiolation at the stages of introducing explants and rooting microcuttings in in vitro culture. The work was carried out in the laboratory of in vitro grape culture of the department of grape nursery, propagation and biotechnology of the National Scientific Centre «V.Ye. Tairov Institute of Viticulture and Winemaking» NAAS of Ukraine according to the generally accepted method on table, technical and grafted varieties of grapes. Etiolation was applied in two stages: introduction of initial grape explants into in vitro culture (for 7, 15, and 25 days) and rooting of grape microcuttings (for 7 and 15 days). The survival and proliferation of explants, the main biometric indicators of growth and development of microclones were determined. It was established that at the stage of introduction of initial explants of grapes into in vitro culture, the use of etiolation for 15 days, and at the stage of micropropagation - for 7 days, had a positive effect on rooting, proliferation of axillary buds and further development of explants and microcuttings. The height of the stem of the experimental microclones was 0.6-2.6 cm higher, and the number of leaves was 0.4-0.9 more than that of the control plants. Also, etiolation had a positive effect on the increase in the length and biomass of roots in microcuttings. In the future, it is planned to analyze changes in the in vitro reproduction coefficient of various grape varieties under the influence of etiolation.

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In vitro propagation of avocado (Persea americana Mill. cv. Hass) through morphogenesis

Cited by 3 publications

References 7 publications

An efficient in vitro organogenesis protocol for the endangered relic tree species Bretschneidera sinensis and genetic fidelity assessment using DNA markers

An efficient in vitro organogenesis protocol for the endangered relic tree species Bretschneidera sinensis and genetic fidelity assessment using DNA markers

Regeneración de explantes de hojas de cinco genotipos de frambuesa

Application of Etiolation of Grape Explants in Vitro Culture

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