Application of Etiolation of Grape Explants in Vitro Culture

Abstract

Micropropagation of grapes is used to obtain original and certified planting material for planting mother plants. The decrease in the efficiency of microclonal reproduction is associated with difficulties at the stages of introducing explants into in vitro culture and rooting. The method of etiolation is simple to use and allows you to prevent phenolic oxidation of explants, has a positive effect on increasing the length of shoots and the number of roots. The purpose of the research was to determine the effectiveness of etiolation at the stages of introducing explants and rooting microcuttings in in vitro culture. The work was carried out in the laboratory of in vitro grape culture of the department of grape nursery, propagation and biotechnology of the National Scientific Centre «V.Ye. Tairov Institute of Viticulture and Winemaking» NAAS of Ukraine according to the generally accepted method on table, technical and grafted varieties of grapes. Etiolation was applied in two stages: introduction of initial grape explants into in vitro culture (for 7, 15, and 25 days) and rooting of grape microcuttings (for 7 and 15 days). The survival and proliferation of explants, the main biometric indicators of growth and development of microclones were determined. It was established that at the stage of introduction of initial explants of grapes into in vitro culture, the use of etiolation for 15 days, and at the stage of micropropagation - for 7 days, had a positive effect on rooting, proliferation of axillary buds and further development of explants and microcuttings. The height of the stem of the experimental microclones was 0.6-2.6 cm higher, and the number of leaves was 0.4-0.9 more than that of the control plants. Also, etiolation had a positive effect on the increase in the length and biomass of roots in microcuttings. In the future, it is planned to analyze changes in the in vitro reproduction coefficient of various grape varieties under the influence of etiolation.