In vitro propagation and field establishment of Eulophia cullenii (Wight) Bl., a critically endangered orchid of Western Ghats, India through culture of seeds and axenic seedling-derived rhizomes

Decruse, S. William; Shylajakumari, S.; Krishnan, P. N.

doi:10.1007/s11627-013-9521-0

Cited by 18 publications

(7 citation statements)

References 15 publications

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“…The number of shoots produced per explant in VW and KC was significantly lower than in ½MS. Decruse et al (2013), upon studying the in vitro propagation of Eulophia cullenii, verified that shoot proliferation was higher on ¼MS and KC than on the other media surveyed. Different results were reported by Duan & Yazawa (1994) for ×Doriella Tiny (Doritis pulcherrima × Kingiella philippinensis).…”

Section: Effect Of Culture Media On Multiplication and Growthmentioning

confidence: 90%

Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro

et al. 2018

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Catasetum macrocarpum is an epiphytic orchid that has been subjected to strong environmental pressure in the state of Tocantins. This investigation aimed at studying the germination, growth and morpho-anatomical development of C. macrocarpum under in vitro conditions. The effects of three culture media on the in vitro germination and on the multiplication and growth of 90-day-old seedlings were studied: Murashige & Skoog (full- and half-strength), Knudson C, and Vacin & Went. The effects of different concentrations of benzyladenine (BA) and naphthaleneacetic acid (NAA) on the multiplication and growth of 120-day-old plants were evaluated. Anatomical studies were conducted on protocorms at different developmental stages. Acclimatization was also carried out. Knudson C was the best medium for seed germination whereas Vacin & Went promoted the greatest protocorm development. Half-strength Murashige & Skoog was the most effective medium for seedling multiplication and growth. The results revealed that 1 mg L-1 BA was the best treatment for shoot proliferation and leaf production. NAA at 0.5 mg L-1 strongly favored root formation. The anatomical study revealed that the early stages of C. macrocarpum development do not always coincide with the morphological phases described. The acclimatization of C. macrocarpum plants provided successful results regarding plant survival.

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Section: Effect Of Culture Media On Multiplication and Growthmentioning

confidence: 90%

Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro

et al. 2018

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“…KN and VW provided very similar outcomes. Upon studying the in vitro propagation of Eulophia cullenii Whight, Decruse et al (2013) also verifi ed that shoot proliferation was higher on diluted MS medium (¼MS) than on the other surveyed media. Diff erent results were reported by Duan & Yazawa (1994) for Doriella Tiny (Doritis pulcherrima Lindl.…”

Section: Eff Ects Of Culture Media Sucrose and Growth Regulators On mentioning

confidence: 97%

“…Although longer shoots were observed on ½MS than on MS, no statistical diff erence was detected between them. Decruse et al (2013) reported that MS was statistically superior to ½MS and KN for shoot length of E. cullenii. Suzuki et al (2009) did not fi nd diff erences for shoot length in Hadrolaelia tenebrosa among MS, VW and KN.…”

Section: Eff Ects Of Culture Media Sucrose and Growth Regulators On mentioning

confidence: 99%

Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Ferreira¹,

Vasconcelos²,

Silva³

et al. 2017

Iheringia Ser Bot

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-This investigation aimed at studying the germination and initial growth of Alatiglossum fuscopetalum (Hoehne) Baptista under in vitro conditions. The eff ects of Murashige/Skoog (MS and ½MS), Knudson (KN), and Vacin/Went (VW) culture media on the in vitro germination of A. fuscopetalum were analyzed. The infl uence of these media on the multiplication and initial growth of 90-day-old seedlings, and of diff erent sucrose, benzyladenine [BA] and indole-butyric acid [AIB] concentrations on the multiplication and growth of 120-day-old plants were also evaluated. The results revealed that ½MS was the most appropriate medium for germination. Regarding the growth of 90-day-old seedlings, MS provided the best outcome. The most eff ective medium for the multiplication and growth of 120-day-old plants was ½MS. Sucrose ranging from 1% to 3% favored plant growth. BA at 1 mg L -1 and IBA at 2 mg L -1 were the best growth regulator treatments for shoot proliferation and root growth, respectively.

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“…However, E. flava plantlets visibly wilted during transfer to ex vitro acclimatization. Related reports found that wilting symptoms caused by temporary dormancy during transplantation could occur in some species, i.e., Eulophia cullenii [35] and Calopogon tuberosus [36]. Plantlets of E. flava from all culture systems were transferred at the acclimatization step to cultivation in artificial streams for a further eight weeks.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

Kunakhonnuruk

Inthima

Kongbangkerd

2019

Biology

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Epipactis flava Seidenf. is an endangered Thai rheophytic orchid that has recently shown a rapid decrease in its natural habitat, prompting an urgent need for conservation using ex situ reintroduction methods. Temporary immersion system (TIS) has been successfully applied for large-scale propagation in various plants species. Propagation efficiency of E. flava using TIS was investigated and compared with conventional semi-solid system (SSS) and liquid continuous immersion system (CIS). The highest percentage of new shoot and shoot bud formation was obtained from TIS, followed by CIS and SSS, respectively. Growth parameters as indicated by number of new shoots, shoot buds, shoot height and leaves per explant were significantly higher using TIS than with SSS and CIS. Moreover, the maximum number of new shoots and shoot buds per replication were reliably obtained from TIS higher than SSS and CIS. After acclimatization, the highest survival percentage of plantlets was observed in TIS (76.7%), with 60% surviving after eight weeks of transplantation in artificial stream. TIS was determined as the most suitable culture system for in vitro mass propagation of E. flava compared to CIS and SSS.

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In vitro propagation and field establishment of Eulophia cullenii (Wight) Bl., a critically endangered orchid of Western Ghats, India through culture of seeds and axenic seedling-derived rhizomes

Cited by 18 publications

References 15 publications

Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro

Germination, growth and morpho-anatomical development of Catasetum macrocarpum (Orchidaceae) in vitro

Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

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