In vitro propagation and bioactive compound accumulation in regenerated shoots of Dioscorea birmanica Prain &amp; Burkill

Jirakiattikul, Yaowapha; Rithichai, Panumart; Songsri, Onouma; Ruangnoo, Srisopa; Itharat, Arunporn

doi:10.1007/s11738-016-2268-6

Cited by 6 publications

(8 citation statements)

References 17 publications

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“…Total phenolic content was measured using the Folin-Ciocalteu method as described in Jirakiattikul et al (2016). Gallic acid was used as the standard.…”

Section: Secondary Metabolite Determinationmentioning

confidence: 99%

“…The DPPH radical scavenging activity of the extracts was determined based on the methods of Yamasaki et al (1994), as described by Jirakiattikul et al (2016). BHT (butylated hydroxyltuluene) was used as the standard.…”

Section: Secondary Metabolite Determinationmentioning

confidence: 99%

“…Plant tissue culture is an alternative technique used to produce secondary metabolites in many medicinal plants (Sauerwein et al 1991;Amoo et al 2012;Tan et al 2010;Jirakiattikul et al 2016). The advantages of this technique over conventional production are independence from environmental factors, continuous sourcing of products of consistent quality and quantity, and production of novel compounds (Ramachandra Rao and Ravishankar 2002).…”

Section: Introductionmentioning

confidence: 99%

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Improvement of dioscorealide B production by elicitation in shoot cultures of Dioscorea membranacea Pierre ex Prain & Burkill

Jirakiattikul

Rithichai

Boonyeun

et al. 2020

Physiol Mol Biol Plants

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Dioscorealide B is an important secondary metabolite isolated from Dioscorea membranacea Pierre ex Prain & Burkill. The effect on secondary metabolite content of different concentrations of two elicitors [jasmonic acid (JA) and salicylic acid (SA)], and of medium status and JA exposure period were investigated. In the JA and SA concentration experiment, 6-week-old shoots were cultured on MS medium supplemented with 8.87 lM BA (6-benzyladenine) in combination with 100-500 lM JA or 50-200 lM SA for 3 weeks. MS medium supplemented only with 8.87 lM BA was used as a control. The highest dioscorealide B content was recorded in the 100 lM JA shoots. To determine the optimal medium status and JA exposure period, shoots were cultured on solid and in liquid MS media supplemented with 8.87 lM BA and 100 lM JA for 2, 3, 4 and 5 weeks. No interaction was found between the medium status and the elicitor exposure period in the dioscorealide B production. Shoots cultured on the solid MS medium supplemented with 100 lM JA had a higher dioscorealide B content (0.57 ± 0.35% w/w) than those cultured in liquid medium (0.36 ± 0.40% w/w) and 5-week JA exposure produced the highest dioscorealide B content of 1.05 ± 0.15% (w/w).

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“…Total phenolic content was measured using the Folin-Ciocalteu method as described in Jirakiattikul et al (2016). Gallic acid was used as the standard.…”

Section: Secondary Metabolite Determinationmentioning

confidence: 99%

Section: Secondary Metabolite Determinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improvement of dioscorealide B production by elicitation in shoot cultures of Dioscorea membranacea Pierre ex Prain & Burkill

Jirakiattikul

Rithichai

Boonyeun

et al. 2020

Physiol Mol Biol Plants

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“…The total phenolic content was determined using Folin-Ciocalteu colorimetric method as described by Jirakiattikul et al [20]. The extract was dissolved in absolute ethanol to a concentration of 1 mg/ml and sonicated for 1 min.…”

Section: Total Phenolic Contentmentioning

confidence: 99%

Enhancement of seed quality and bioactive compound accumulation in sunflower sprouts by dielectric barrier discharge plasma treatment

Rithichai¹,

Jirakiattikul²,

Singhawiboon³

et al. 2021

ScienceAsia

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Dielectric barrier discharge (DBD) plasma treatment has been reported to increase the quality of seeds and seedlings. In order to improve sprout quality, this study was conducted to investigate the effect of DBD plasma treatment on seed germination, vigour, growth, and bioactive compound content of sunflower (Helianthus annuus) sprouts. Two sunflower cultivars, Arfael and Jumbo, were treated using atmospheric DBD plasma at a discharge voltage of 20 kV for 15, 30, 60, 120, and 240 s. The non-treated seeds were used as control. The responses of seeds to the treatment depended on the exposure time and the cultivar. While the treatment stimulated seed germination and improved the vigour of the Jumbo cultivar at all exposure times, the Arfael cultivar responded only to exposures of 60 s, 120 s, and 240 s. Sprouts derived from 60 s, 120 s, and 240 s DBD plasma treatments exhibited greater dry weight than those from other treatments. Moreover, the Arfael sprouts showed a higher bioactive compound level than the Jumbo sprouts. The DBD plasma treatments of both cultivars for 120 s and 240 s enhanced the accumulation of total phenolics and flavonoids. However, plasma treatment did not affect DPPH radical scavenging. The results from this study indicate that the 120 s DBD plasma treatment is effective in enhancing seed germination, growth, and bioactive compound of sunflower sprouts. This study is the first of its kind to illustrate the potential benefits of the DBD plasma treatment to enhance the quality of sunflower sprouts.

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“…In vitro tissue culture has been widely used for rapid plant propagation and obtaining bioactive compounds from cell culture [33]. There are previous studies of a successful induction and propagation of calluses of C. ensiformis under in vitro conditions [34].…”

Section: Introductionmentioning

confidence: 99%

Preliminary study of the production of metabolites from in vitro cultures of C. ensiformis

2020

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Background Canavalia ensiformis is a legume native to Central and South America that has historically been a source of protein. Its main proteins, urease, and lectin have been extensively studied and are examples of bioactive compounds. In this work, the effect of pH and light effects on the growth of C. ensiformis were analyzed. Also, the bioactive compounds such as phenols, carotenoids, chlorophyll a/b, and the growth of callus biomass of C. ensiformis from the effect of different types of light treatments (red, blue and mixture) were evaluated. Likewise, the antioxidative activity of C. ensiformis extracts were studied and related to the production of bioactive compounds. For this, a culture of calluses obtained from seeds were carried out. For the light experiments, polypropylene boxes with red, blue, combination (1/3, 3/1 and 1/1 R-B, respectively) lights and white LED were used as control. In each treatment, three glass containers with 25 ml of MS salts containing 0.25 g of fresh callus were seeded. Results The results have shown that the pH of the culture medium notably affects the increase in callogenic biomass. It shows that the pH of 5.5 shows better results in the callogenic growth of C. ensiformis with an average increase of 1.3051 g (198.04%), regarding the initial weight. It was found that the pH 5.5 and the 1/3 R-B LED combination had higher production of bioactive compounds and better antioxidant activity. At the same time, the red-light treatment was least effective. Conclusions It was possible to find the ideal conditions of important growth under conditions of pH and light of C. ensiformis. Likewise, it is evaluated whether the production of compounds of interest, such as phenolic compounds and carotenoids, occurs under these conditions. The highest production of calluses occurs in the 1/3 R-B LED combined light treatment, which showed a significant increase in biomass, followed by B. From this study, it could be demonstrated that C. ensiformis produces compounds such as phenols and carotenoids in vitro culture that are essential for the antioxidant activity of the plant.

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In vitro propagation and bioactive compound accumulation in regenerated shoots of Dioscorea birmanica Prain & Burkill

Cited by 6 publications

References 17 publications

Improvement of dioscorealide B production by elicitation in shoot cultures of Dioscorea membranacea Pierre ex Prain & Burkill

Improvement of dioscorealide B production by elicitation in shoot cultures of Dioscorea membranacea Pierre ex Prain & Burkill

Enhancement of seed quality and bioactive compound accumulation in sunflower sprouts by dielectric barrier discharge plasma treatment

Preliminary study of the production of metabolites from in vitro cultures of C. ensiformis

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