Improvement of dioscorealide B production by elicitation in shoot cultures of Dioscorea membranacea Pierre ex Prain & Burkill

Abstract

Dioscorealide B is an important secondary metabolite isolated from Dioscorea membranacea Pierre ex Prain & Burkill. The effect on secondary metabolite content of different concentrations of two elicitors [jasmonic acid (JA) and salicylic acid (SA)], and of medium status and JA exposure period were investigated. In the JA and SA concentration experiment, 6-week-old shoots were cultured on MS medium supplemented with 8.87 lM BA (6-benzyladenine) in combination with 100-500 lM JA or 50-200 lM SA for 3 weeks. MS medium supplemented only with 8.87 lM BA was used as a control. The highest dioscorealide B content was recorded in the 100 lM JA shoots. To determine the optimal medium status and JA exposure period, shoots were cultured on solid and in liquid MS media supplemented with 8.87 lM BA and 100 lM JA for 2, 3, 4 and 5 weeks. No interaction was found between the medium status and the elicitor exposure period in the dioscorealide B production. Shoots cultured on the solid MS medium supplemented with 100 lM JA had a higher dioscorealide B content (0.57 ± 0.35% w/w) than those cultured in liquid medium (0.36 ± 0.40% w/w) and 5-week JA exposure produced the highest dioscorealide B content of 1.05 ± 0.15% (w/w).

Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview

“…An increased accumulation of anti-inflammatory alkaloids (pteropodine, isopteropodine, speciophylline, rumberine, hameline and palmirine) was reported in JA elicited Hamelia patens ( Flores-Sanchez et al, 2016 ). The highest dioscorealide B (a phenolic compound) contentwas recorded in the 100 μM JA elicited in vitro shoots of Dioscorea membranacea ( Jirakiattikul et al, 2020 ).…”

Enhanced in vitro production of diosgenin in shoot cultures of Dioscorea deltoidea by elicitation and precursor feeding

“…The results were inconsistent with Hypericum hirsutum and Hypericum maculatum shoot cultures, in which jasmonic acid (JA) at higher concentration signi cantly inhibited biomass production and SA in uenced biomass production slightly in relation to control [34]. Recently, Jirakiattikul et al [35] also indicated JA to have minor effect on D. membranacea shoot cultures, whereas SA demonstrated no effect at all. In another study, it was found that MeJa reduced the growth of Centella asiatica culture at concentrations above 0.1 mM [36].…”

“…The production of psoralen was enhanced when MeJa and SA at 100 µM were added to the suspension culture of Psoralea corylifolia [42]. Therefore, MeJa has been well established as a key signal compound which was positively involved in the signal transduction pathway that leads to secondary metabolite production [43,35]. Diosgenin production was also favoured by SA which is a widely studied signaling molecule to trigger secondary metabolite production in plants [44].…”

Enhanced in vitro production of diosgenin in shoot cultures of Dioscorea deltoidea by elicitation and precursor feeding

“…The results were inconsistent with Hypericum hirsutum and Hypericum maculatum shoot cultures, in which jasmonic acid (JA) at higher concentration signi cantly inhibited biomass production and SA slightly effected biomass production relative to control [34]. Recently, Jirakiattikul et al [35] also indicated that, JA had low effect on D. membranacea shoot cultures, whereas SA had no effect. In an another study, it was found that MeJa reduced the Centella asiatica culture growth at concentration above 0.1 mM [36].…”

“…The production of psoralen was enhanced when MeJa and SA at 100 µM was added to the suspension culture of Psoralea corylifolia [42]. Therefore, MeJa have been well established as key signal compounds and positively involved in the signal transduction pathway that leads to secondary metabolite production [43,35]. Diosgenin production was also favoured by SA which is widely studied signaling molecule to trigger secondary metabolite production in plants [44].…”