In vitro production of potato microtubers in liquid medium using temporary immersion

Abstract

SummaryCIRAD developed a new apparatus for plant tissue culture, using temporary immersion in a liquid medium. This apparatus was adapted to the microtuber production in potato. The procedure is as follows: single node cultivation on MS medium containing 30 g/l sucrose in the light for 2 weeks, induction of microtuberisation with 80 g/l sucrose over a 2 week period in the light, followed by a further 6 weeks in the dark. All experiments were performed at 20 ~ The basic vessel had a capacity of approximately 1 I; 30 nodes were cultivated per vessel. Depending on the cultivars tested (Bintje, Ostara and D6sir6e) 47 to 115 microtubers were harvested per vessel. Between 30 and 60% of the microtubers weighted over 0.5 g and between 10 and 40% over 0.8 g. Sprouting is still under investigation. Preliminary results indicate that the dormancy period was relatively short and several stems were obtained per microtuber. These results seem to be better than those usually reported. Only one simple protocol has been tested and further improvements are probably easy to obtain.

Optimization of immersion frequency and medium substitution on microtuberization of Chlorophytum borivilianum in RITA system on production of saponins

“…The 0.9 liter RITA ® vessels were adjusted to twin flask system, as described in previous study on potato [11]. In vitro rooted shoots of C. borivilianum (2.0-2.5 cm in length with initial mean weight of 1.3 g), taken from cultures maintained on solid MS medium [12] without plant growth regulators, were used as explants.…”

Temporary immersion systems in plant biotechnology

“…One of the containers has the function of a culture chamber, whereas the other container is used as a medium storage tank. The culture chamber container may or may not be equipped with support material for explants (glass beads, polyurethane foam, metal or nylon sieves may be used) at its bottom [21,22,[24][25][26][27][28][29][30]. Each container is connected to its own pressurized-air line, controlled by two independent timer clocks, coupled with three-way solenoid valves.…”

Jasmonate effect on in vitro tuberization of potato (Solanum tuberosum L.) cultivars under light and dark conditions

“…The constraints of poor tuber uniformity, small size and low progeny yield, which might restrict their use as propagules of choice (Estrada et al, 1986;Struik & Lommen, 1990), are being overcome by manipulation of the tuberization conditions (Nowak & Asiedu, 1992;Struik & Wiersema, 1999) and the introduction of a bioreactor technology (Jimenez et al, 1999;Teisson & Alvard, 1999;Yu et al, 2000). Microtubers can be easily produced in vitro and could be used in greenhouse production of minitubers (nuclear seed) at the start of seed programs (Lommen, 1995).…”