Optimization of immersion frequency and medium substitution on microtuberization of Chlorophytum borivilianum in RITA system on production of saponins

Ashraf, Mehdi Farshad; Aziz, Maheran Abdul; Stanslas, Johnson; Kadir, Mihdzar Abdul

doi:10.1016/j.procbio.2012.12.001

Cited by 21 publications

(9 citation statements)

References 28 publications

(31 reference statements)

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“…Twin‐Flask systems have been successfully applied in the propagation of plant seedlings, shoots, nodule cluster, and embryo cultures . Recently, Twin‐Flask systems have been used in research work on secondary metabolites accumulation by differentiated in vitro cultures as well .…”

Section: Design and Operation Of Tismentioning

confidence: 99%

“…One of the containers has the function of a culture chamber, whereas the other container is used as a medium storage tank. The culture chamber container may or may not be equipped with support material for explants (glass beads, polyurethane foam, metal or nylon sieves may be used) at its bottom [21,22,[24][25][26][27][28][29][30]. Each container is connected to its own pressurized-air line, controlled by two independent timer clocks, coupled with three-way solenoid valves.…”

Section: Twin-flask Systemmentioning

confidence: 99%

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Temporary immersion systems in plant biotechnology

Georgiev

Schumann

Pavlov

et al. 2014

Engineering in Life Sciences

157

111

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Plant tissue and organ cultures in vitro usually face technological challenges. When submerged cultivation of plant cells in a controlled environment is desired, the characteristic growth morphology and physiology of differentiated organ cultures present a problem in process scale‐up. Temporary immersion systems (TIS) were developed several decades ago. These systems are providing the most natural environment for in vitro culture of plant shoots and seedlings. Over the past few years, TIS have been recognized as a perspective technology for plant micropropagation, production of plant‐derived secondary metabolites, expression of foreign proteins, and potential solutions in phytoremediation. Nowadays, several TIS, operating on similar or divergent technological principles, have been developed and successfully applied in the cultivation of various plant in vitro systems, including somatic embryos and transformed root cultures. In this article, the operational principle and technological design of the most popular TIS are reviewed. In addition, recent examples of the application of temporary immersion technology for in vitro cultivation of plant tissue and organ cultures at laboratory and pilot scales are discussed. Finally, future prospects and challenges to the industrial realization of that fast‐developing technique are outlined.

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Section: Design and Operation Of Tismentioning

confidence: 99%

Section: Twin-flask Systemmentioning

confidence: 99%

Temporary immersion systems in plant biotechnology

Georgiev

Schumann

Pavlov

et al. 2014

Engineering in Life Sciences

157

111

View full text Add to dashboard Cite

show abstract

“…Purohit et al [11] used young shoot bases as explants, even though shoot regeneration through immature inflorescence cultures was reported by Samantaray et al [12]. Latest study reported microtuberization of C. borivilianum by using RITA system with saponin enhancement of in vitro tubers [13,14], but there is no sufficient study on a rapid method for shoot multiplication and elongation. This study reports on the attainment of improved shoot multiplication and elongation of C. borivilianum based on the interaction between the cytokinins BAP and Kn.…”

Section: Introductionmentioning

confidence: 99%

Effect of cytokinin types, concentrations and their interactions on in vitro shoot regeneration of Chlorophytum borivilianum Sant. & Fernandez

Ashraf

Aziz

Kemat

et al. 2014

Electronic Journal of Biotechnology

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Background: Chlorophytum borivilianum is a rare medicinal plant originally distributed throughout the forest of India. The tubers of C. borivilianum are used as an aphrodisiac and impotence supplement. The propagation of C. borivilianum is possible through seeds and tubers, but conventional methods may take several months. Hence in vitro technique of shoot regeneration could be an efficient alternative means of propagating the species. Latest study reported microtuberization of C. borivilianum but there is no sufficient study on a rapid method for shoot multiplication and elongation. Results: Young shoot buds of C. borivilianum were cultured on MS medium containing 6-benzylaminopurine (BAP) and Kinetin (Kn), both at 0, 8.88, 17.8 and 26.6 μM, either individually or in combinations. Proliferated shoots were subcultured on fresh medium of the same constituents on week 3 of culture for further shoot multiplication and elongation. BAP alone (8.88-26.6 μM) was significantly effective on shoot multiplication, while Kn alone (8.88-26.6 μM) was significantly effective on shoot elongation compared to the control containing MS basal medium without any plant growth regulator. However, combination of both cytokinins stimulated an interaction producing higher shoot number and shoot length compared to their individual application. Conclusions: The most suitable combination was 8.88 μM BAP + 8.88 μM Kn, reaching a mean shoot number of 10.83 and shoot length of 6.85 cm.

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“…This was expected because of the better availability of nutrients as well as the faster and efficient uptake of nutrients from the liquid medium in the TIS 28 . Some other medicinal plant species were also successfully micropropagated via TIS [29][30][31] .…”

Section: Resultsmentioning

confidence: 99%

Untitled

2014

IJPSR

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Arnica montana L. (Asteraceae) is a valuable medicinal plant species, endemic to Europe, which is threatened in many countries due to its overharvesting. The increasing market demand requires development of an effective method for A. montana rapid propagation, offering the possibility for its field cultivation as an alternative to plant gathering from nature. Three invitro culture systems were compared to determine the best shoot multiplication of A. montana: agar-gelled medium, static liquid medium and Temporary Immersion System (TIS RITA ® ). Murashige and Skoog (MS) nutrient medium supplemented with 1 mg/l BA and 0.1 mg/l IAA was used in all experiments. The highest micropropagation rate (18.2 shoots/explant, for 5 weeks) was obtained via TIS culture. Besides, these plants showed higher sesqiuterpene lactones content than those derived from the other tested systems. The shoots were successfully in -vitro rooted and ex-vitro adapted. The elaborated method for mass multiplication of A. montana allows significant enhancement of the invitro process and could be applied to produce planting material, thus contributing to the conservation of the species as well. QUICK RESPONSE CODE

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Optimization of immersion frequency and medium substitution on microtuberization of Chlorophytum borivilianum in RITA system on production of saponins

Cited by 21 publications

References 28 publications

Temporary immersion systems in plant biotechnology

Temporary immersion systems in plant biotechnology

Effect of cytokinin types, concentrations and their interactions on in vitro shoot regeneration of Chlorophytum borivilianum Sant. & Fernandez

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