In vitro production of erythropoietin by mouse fetal liver

Zucali, JR; Stevens, V. C.; Mirand, E. A.

doi:10.1182/blood.v46.1.85.85

Cited by 38 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, macrophages present in the culture can produce and release Epo. This has been shown for fetal liver cells (Zucali & Mirand, 1975;Rich et al, 1980) and indeed for most macrophage-containing cell suspensions, including spleen and bone marrow (Rich et al, , 1981. Low, but constant, amounts of Epo produced by macrophages in vitro could account for the observed effects produced by conditioned media, particularly when substances are present which could stimulate macrophages.…”

Section: Discussionmentioning

confidence: 79%

The effect of reduced oxygen tension on colony formation of erythropoietic cells in vitro

Rich

Kubanek

1982

Br J Haematol

View full text Add to dashboard Cite

The effect of reduced oxygen tension and the role of cellular components known to protect the cell against oxygen toxicity has been studied with respect to erythropoietic colony formation in vitro. Alphathioglycerol can be partially replaced by vitamin E and completely replaced by reduced glutathione (GSH) at physiological concentrations. Incubation of bone marrow and fetal liver early (BFU-E) and late (CFU-E) erythropoietic progenitor cells, in the presence of GSH, in an atmosphere containing 5% oxygen, 5% carbon dioxide and 90% nitrogen, as opposed to air supplemented with 5% carbon dioxide, resulted in an increase in colony numbers and response to erythropoietin (Epo). The number of colonies derived from bone marrow and fetal liver CFU-E increased by 1.2--2.8-fold with a relative Epo sensitivity increase of 3.5--4-fold. Bursts obtained from bone marrow and fetal liver BFU-E increased from 2.6- to 3.8-fold with an increased response to Epo of 2--3-fold. The effects of GSH and low oxygen tension are interpreted as causing a reduction in oxygen toxicity of the cells, thereby increasing the life span in vitro and so increasing the number of cells capable of forming colonies. The heightened response of BFU-E to Epo, analogous to the effect seen for CFU-E, implies that BFU-E may be responsive to physiological Epo concentrations at physiological oxygen tensions.

show abstract

Section: Discussionmentioning

confidence: 79%

The effect of reduced oxygen tension on colony formation of erythropoietic cells in vitro

Rich

Kubanek

1982

Br J Haematol

View full text Add to dashboard Cite

show abstract

“…Epo is produced in the murine fetal liver ( Jacobs et al , 1985 ; Koury et al , 1991 ). Since unfractionated liver cells were cultured for this study, it is possible that the Epo‐independent CFU‐E growth and the SCF‐dependent BFU‐E growth described here were mediated by Epo released in the culture by the accessory cells ( Zucali et al , 1975 ). This possibility was excluded, at least for SCF, by comparing its effects on the proliferation and differentiation of progenitors cells purified from FL and AM (CFU‐E are not enriched by the purification strategy used in this study; Migliaccio et al , 1988 ).…”

Section: Discussionmentioning

confidence: 99%

Stem cell factor induces proliferation and differentiation of fetal progenitor cells in the mouse

et al. 1998

View full text Add to dashboard Cite

Summary.We have investigated the kinetics of the amplification of the progenitor cell compartments (CFC) in haemopoietic organs during murine ontogenesis and compared the growth requirements of fetal and adult CFC. Two haemopoietic phases were recognized in the fetal liver (FL): an exponential growth phase, from 11·5 to 15·5 d post conception (p.c.), during which the mean number of nucleated cells and of CFC in the FL increased from 4·9 × 10 5 to 7·0 × 10 7 and from 4·5 × 10 3 to 2·7 × 10 5 , respectively, and a recessive phase after 15·5 d p.c., during which the CFC number in the FL gradually decreased, although some CFC were still detectable in the liver after birth. In serum-deprived cultures, FL and adult marrow (AM) CFC had similar responses to GM-CSF, and did not respond to G-CSF or IL-3. In contrast, FL, but not AM, erythroid colonies grew Epo-independently whereas SCF alone induced formation of maximal numbers of erythroid bursts from FL, but not from AM cells. The proliferative and differentiative effect of SCF alone on fetal cells was confirmed in serum-deprived cultures of purified early progenitor cells isolated by cell sorting on the basis of multiple parameters from FL and AM light-density cells. In culture of purified FL cells, SCF alone induced a similar amplification of total cells (maximal amplification at day 12: 800-300-fold) and total CFC (11-38-fold of maximal amplification at day 6) to the combination of SCF plus IL-3 (1300-800-fold amplification of total cells and 31-88-fold amplification of CFC). In contrast, SCF alone allowed only survival of purified AM early progenitor cells. Therefore FL early progenitor cells have an intrinsic higher potential than their adult counterpart to respond to SCF, confirming the potent role of this growth factor in the development of the murine haemopoietic system.

show abstract

“…When he exposed the cells to a low O2 environment, the culture media contained significantly elevated erythropoietin levels when measured in the polycythaemic mouse assay. Burlington et al (1972) have also reported on the production of erythropoietin from goat renal glomeruli cultures and Zucali & Mirand (1975) have demonstrated the production of an erythropoietically active substance from cultures of mouse fetal liver. These studies, therefore, certainly point to the feasibility of using cell culture systems in studies of the regulation of erythropoietin production.…”

mentioning

confidence: 99%

A Controlled Environmental Culture System for the Production of Erythropoietin

Zucali

Mirand

1976

Scandinavian Journal of Haematology

View full text Add to dashboard Cite

A controlled environmental culture system in which consistent growth of fetal liver cells typical of the tissue of origin and capable of producing erythropoietin in culture is described. This system attempts to duplicate the major components of the natural habitat of the mammalian cell allowing for meaningful studies to be conducted on the regulation and control of erythropoietin biosynthesis. The direct effects of changes in pO2, pCO2, pH and various cell nutrients on cells producing erythropoietin is now possible.

show abstract

In vitro production of erythropoietin by mouse fetal liver

Cited by 38 publications

References 20 publications

The effect of reduced oxygen tension on colony formation of erythropoietic cells in vitro

The effect of reduced oxygen tension on colony formation of erythropoietic cells in vitro

Stem cell factor induces proliferation and differentiation of fetal progenitor cells in the mouse

A Controlled Environmental Culture System for the Production of Erythropoietin

Contact Info

Product

Resources

About