A Controlled Environmental Culture System for the Production of Erythropoietin

Zucali, James R.; Mirand, E. A.

doi:10.1111/j.1600-0609.1976.tb01133.x

Scandinavian Journal of Haematology

1976

DOI: 10.1111/j.1600-0609.1976.tb01133.x

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A Controlled Environmental Culture System for the Production of Erythropoietin

James R. Zucali

E. A. Mirand

Abstract: A controlled environmental culture system in which consistent growth of fetal liver cells typical of the tissue of origin and capable of producing erythropoietin in culture is described. This system attempts to duplicate the major components of the natural habitat of the mammalian cell allowing for meaningful studies to be conducted on the regulation and control of erythropoietin biosynthesis. The direct effects of changes in pO2, pCO2, pH and various cell nutrients on cells producing erythropoietin is now pos… Show more

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Cited by 5 publications

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Exceptionally high serum erythropoietin activity in an anephric patient with severe anemia

Ortega¹,

Malekzadeh²,

Dukes³

et al. 1977

American J Hematol

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An exceptionally high serum erythropoietin (EPO) activity was documented in an anephric patient with severe anemia who required transfusions every 4 weeks. The patient's serum EPO was comparable to normal human urinary EPO in the polycythemic mouse assay and was neutralized by an antiserum against EPO. The patient's serum inhibited EPO stimulated-heme synthesis by normal human marrow cells in vitro. This finding suggests that an inhibitor played an important role in causing the anemia of this uremic patient.

show abstract

Exceptionally high serum erythropoietin activity in an anephric patient with severe anemia

Ortega¹,

Malekzadeh²,

Dukes³

et al. 1977

American J Hematol

View full text Add to dashboard Cite

show abstract

Production of anchorage‐dependent cells—problems and their possible solutions

Jensen

1981

Biotech & Bioengineering

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In vitro cell culture has proven to be a valuable procedure for the study of cellular function as well as the production of veterinary and human vaccines. In recent years, interest in cell culture technology has grown due to the need for production of interferon, monoclonal antibodies, various hormones, and other cell‐secreted products with potential medical uses. Many of these products have been successfully produced only be cells which require attachment to a surface in order to grow and function. This requirement places severe limitations on the economics and feasibility of large‐scale culture. In response, a number of new technologies have been proposed and developed in attempts to increase the surface area available within a given culture volume, to increase the cell density per surface area, to decrease medium and serum requirements per unit of product, to increase product production by control of the cellular environment, and to automate the production process. Some of the techniques and described along with their ability to control the environment of cells in culture.

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Production of erythropoietin in vitro: A review

1978

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The in vitro production of the important regulator of erythropoiesis, erythropoietin (Epo), is reviewed. It is concluded that it is possible to produce almost routinely small quantities of Epo in tissue culture. Although such procedures offer the potential to provide large quantities of the hormone for clinical use, the optimum culture conditions and mechanisms for triggering Epo production have yet to be resolved.

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A Controlled Environmental Culture System for the Production of Erythropoietin

Cited by 5 publications

References 7 publications

Exceptionally high serum erythropoietin activity in an anephric patient with severe anemia

Exceptionally high serum erythropoietin activity in an anephric patient with severe anemia

Production of anchorage‐dependent cells—problems and their possible solutions

Production of erythropoietin in vitro: A review

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