In vitro production of cattle×buffalo hybrid embryos using cattle oocytes and African buffalo (Syncerus caffer caffer) epididymal sperm

Owiny, O. D.; Barry, D.M.; Agaba, Morris; Godke, R.A.

doi:10.1016/j.theriogenology.2008.10.016

Cited by 12 publications

(13 citation statements)

References 38 publications

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“…However, the biological usefulness of oocytes reduces during further embryonic development with interspecific extracorporeal fertilization (even with closely related species) [17]. One solution to the problem is adjustment of the method depending on the specificity of source gametes and embryos.…”

Section: Abstract European Bison or Wisent (Bison Bonasus)mentioning

confidence: 99%

“…Typically, the schemes developed for domestic animals are used for extracorporeal hybridization involving the gametes of rare and wild species as basic IVM (in vitro maturation)/IVF (in vitro fertilization)/IVC (in vitro culture) protocols [17][18][19]. Own technology for producing embryos in vitro for wisent has not been modeled yet.…”

Section: Abstract European Bison or Wisent (Bison Bonasus)mentioning

confidence: 99%

“…Domestic cattle (Bos taurus) is the leader in the efficient and large-scale obtaining of embryos in vitro. There is evidence of extracorporeal fertilization of bovine oocytes by the semen of different species [6][7][8][9], including closely related ones: gaur (Bos gaurus), banteng (B. javanicus) [5], yak (B. grunniens) [5,18], African (Syncerus caffer caffer) and Asian (Bubalus bubalis) buffalo [17,19], and American bison (Bison bison) [23].…”

Section: Abstract European Bison or Wisent (Bison Bonasus)mentioning

confidence: 99%

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IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

Сингина¹,

Багиров²,

Danch³

et al. 2016

S-h. biol.

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A b s t r a c t European bison or wisent (Bison bonasus)is a rare species that is endangered. The technology for producing in vitro embryos using oocytes of cows and wisent semen can be an effective tool for conservation of genetic resources of this species and their rational use in basic research on the physiology of development and to create the new animals' types. However, the methods for production of in vitro embryos using the above-mentioned germ cells are currently not described in the literature. In this work, we first attempted to obtain the hybrid cow-wisent embryos in vitro using the IVM/IVF/IVC protocol, developed for domestic cattle (Bos taurus). Frozen epididymal sperm derived from wisent was thawed and prepared by the swim-up procedure. Matured oocytes were coincubated during 18 h together with prepared sperm in Fert-Talp medium, which was replaced then by embrional medium where the embryos were cultured until the blastocyst state. Additionally, for positive control, a part of matured bovine oocytes were fertilized with frozen/thawed ejaculated bull sperm. Fertilizing capacity of male cells of both species was assessed according to the nature of spermatozoa-egg interactions, as well as the capacity of fertilized bovine oocytes to subsequent embryonic development. It was found that the rate of oocytes with sing polyspermy was higher for fertilization with wisent sperm comparing to allogenic insemination (21.6 vs. 8.5 %, P < 0.05). There were no differences between two types of fertilizations nether for the sperm penetration rate (90.0±0.3 vs. 93.3±1.7 % for bull and wisent, respectively), nor for the rate of normal fertilization (78.3±1.7 % vs. 73.2±2.3 %). The similar cleavage and blastocyst formation rates were observed for two fertilization types. Our data indicate the similarity in the mechanisms of oocyte activation and embryonic development in cow and wisent females. In addition, it is obvious that IVM/IVF/IVP protocol, in general, allows in vitro producing cattle-wisent hybrid embryos, however, high levels of polyspermy observed in heterogeneous fertilization, indicates the necessity to adjust the method.Keywords: cattle oocytes, the European bison sperm, fertilization in vitro, hybrid embryos European bison, or wisent, (Bison bonasus) is a rare endangered species. Current European bison population is strongly inbred. All modern wisents come from 12 individuals kept in the zoos and reserves in the early XX century [1]. Low genetic variability is one of the main threats to the long-term maintenance of the species. Assisted reproductive technology such as production of in vitro embryos using oocytes of domestic cattle and wisent semen will not only help to maintain the existing genetic resources of the species, but also to involve this species in basic research on the physiology of development and use in creations of new bred forms [2][3][4].Cow oocytes are a universal object for interspecific hybridization. In publications, there is evidence of their in vitro fertilization using semen of gaur...

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Section: Abstract European Bison or Wisent (Bison Bonasus)mentioning

confidence: 99%

Section: Abstract European Bison or Wisent (Bison Bonasus)mentioning

confidence: 99%

Section: Abstract European Bison or Wisent (Bison Bonasus)mentioning

confidence: 99%

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IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

Сингина¹,

Багиров²,

Danch³

et al. 2016

S-h. biol.

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“…Interspecies hybridization occurs between horse and donkey, domestic cattle and at least five other species; American bison (Bison bison), Yak (Bos grunniens), Banteng (Bos banteng), Sheep (Ovis aries) and Goat (Capra hircus). Birth of cattle (Bos taurus and Bos indicus) x buffalo (Bubalus bubalis) hybrid has reportedly occurred in Russia and China (Owiny et al 2009). European and Asian domestic cattle readily produce fertile hybrids with greater heat tolerance and disease resistance than that of Bos taurus.…”

Section: Introductionmentioning

confidence: 99%

In vitro maturation of buffalo oocytes and fertilization by cattle spermatozoa

Khandoker

Reza

Asad

et al. 2012

Bang. J. Anim. Sci.

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The present research was undertaken to explore the maturation of buffalo oocytes using bovine follicular fluid (BFF) and bovine serum albumin (BSA), as well as subsequent fertilization using cattle spermatozoa. The cumulus oocyte complexes (COCs) were collected by aspiration of slaughterhouse buffalo ovaries. Maturation was performed in TCM 199 supplemented with 10% BFF, 5% BSA or without supplementation (control). The COCs were fertilized in Brackett and Oliphant (BO) medium using capacitated fresh cattle spermatozoa. It was observed that the percentage of COCs reached to M-II stages were 40.78±3.84, 65.74±2.39 and 67.52±0.85; normal fertilization (formation of 2 pronuclei) were 23.28±3.00, 29.30±0.73 and 30.52±1.21 for control, 10% BFF and 5% BSA supplementation, respectively. The supplementation of BFF (10%) and BSA (5%) were given similar results on maturation and increased significantly (p<0.05) than that of the control. It was observed that cattle spermatozoa were fertilized by the buffalo oocytes and the fertilization rate was 23.28% to 30.52% in BFA and BSA supplemented media, respectively. It can be concluded that buffalo oocytes might be fertilized using capacitated cattle spermatozoa and both 10% BFF and 5% BSA could be supplemented in maturation media to enhance the maturation rate as well as fertilization of buffalo oocytes.

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“…However, this methodology results in the production of nuclear-cytoplasmic hybrids [2,3]. Despite numerous attempts in a wide variety of species, the number of live births of cloned offspring is still limited in instances that combine genetic compartments of closely related species [4,5]. The generation of animals through interspecies/intergeneric SCNT (iSCNT) poses several problems including mitochondrial/genomic DNA incompatibility, embryonic genome activation of the donor nucleus by the recipient oocyte, and availability of suitable foster mothers for iSCNT embryos.…”

mentioning

confidence: 99%

Influence of Intergeneric/Interspecies Mitochondrial Injection; Parthenogenetic Development of Bovine Oocytes after Injection of Mitochondria Derived from Somatic Cells

Takeda

Srirattana

Matsukawa

et al. 2012

Journal of Reproduction and Development

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Abstract. Interspecies/intergeneric mitochondrial heteroplasmy can occur in interspecies/intergeneric hybrid embryos or following nuclear transfer. In the present study, intergeneric buffalo (Bubalus bubalis) mitochondria (WB-mt) or interspecies murine (Mus spretus) mitochondria (M-mt) were injected into bovine (Bos taurus) oocytes, and the subsequent embryonic development was characterized. Fibroblast mitochondria (WB-mt or M-mt) were microinjected into in vitro matured bovine oocytes followed by oocyte activation by a combination of electrical stimulation and 6-dimethylaminopurine treatment. After seven days of culture, embryo development was evaluated. The copy number of specific mtDNA populations (introduced and native mtDNA) from heteroplasmic oocytes was estimated using real-time PCR. The results illustrated that oocytes injected with either WB-mt or M-mt can develop to the blastocyst stage (20.6% and 19.6%). Cleavage division rates and development to the morula stage in oocytes injected with WB-mt were lower (76.2% and 45.9%, respectively) in comparison with uninjected oocytes (89.2% and 59.1%, respectively) (P<0.05). However, no differences were found in comparing M-mt injected oocytes and controls (P>0.05). An increase in bovine mtDNA copy number was observed at the expanded blastocyst stage of injected embryos (P<0.01), while the number of injected mtDNA was stable throughout development. This study demonstrates that interspecies/intergeneric mitochondrial injected bovine oocytes have the ability to develop to the blastocyst stage after parthenogenetic activation and that injected mtDNA was neither selectively destroyed nor enhanced through development. Moreover, injected intergeneric mitochondria had a demonstrated influence on bovine parthenogenetic development and mtDNA replication. Key words: Bovine oocyte, Mitochondria, Mouse, mtDNA, Water buffalo (J. Reprod. Dev. 58: [323][324][325][326][327][328][329] 2012) S omatic cell nuclear transfer (SCNT) offers the possibility of preserving endangered species, and SCNT has been applied to many mammalian species [1]. However, owing to the limited availability of oocytes from wild animals, the cloning of endangered species benefits from the use of recipient oocytes from a related domestic species. However, this methodology results in the production of nuclear-cytoplasmic hybrids [2,3]. Despite numerous attempts in a wide variety of species, the number of live births of cloned offspring is still limited in instances that combine genetic compartments of closely related species [4,5]. The generation of animals through interspecies/intergeneric SCNT (iSCNT) poses several problems including mitochondrial/genomic DNA incompatibility, embryonic genome activation of the donor nucleus by the recipient oocyte, and availability of suitable foster mothers for iSCNT embryos. The species-specific nature of mitochondrial biogenesis and function makes this modeling particularly significant for iSCNT.F1 hybrids and iSCNT live offspring obtained using river buffalo (Bubal...

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In vitro production of cattle×buffalo hybrid embryos using cattle oocytes and African buffalo (Syncerus caffer caffer) epididymal sperm

Cited by 12 publications

References 38 publications

IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

IN VITRO PRODUCTION OF INTERSPECIES HYBRID EMBRYOS OF CATTLE (Bos taurus) AND WISENT (Bison bonasus)

In vitro maturation of buffalo oocytes and fertilization by cattle spermatozoa

Influence of Intergeneric/Interspecies Mitochondrial Injection; Parthenogenetic Development of Bovine Oocytes after Injection of Mitochondria Derived from Somatic Cells

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