In vitro probiotic properties of Lactobacillus fermentum SK5 isolated from vagina of a healthy woman

Kaewnopparat, Sanae; Dangmanee, Nattakan; Kaewnopparat, Nattha; Srichana, Teerapol; Chulasiri, Malyn; Settharaksa, Sukanya

doi:10.1016/j.anaerobe.2013.04.009

Cited by 102 publications

(81 citation statements)

References 34 publications

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“…It was reported that coaggregation abilities may allow lactic acid bacteria strains inhibit the growth of pathogenic strains in the gastrointestinal and urogenital tracts in a very close proximity (Botes et al, 2008). Moreover, lactic acid bacteria strains could control a microenviroment around the pathogens and increase the concentration of excreted antimicrobial substances in the process of coaggregating (Kaewnopparat et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…LABs (Lactic acid bacteria) are normal inhabitants of human gastrointestinal tract and play a quite important role in the maintenance of the colonic microbial ecosystem (Kirtzalidou et al, 2011). Lactic acid bacteria could exert beneficial effect on humans due to their potential health-promoting properties, such as inhibition on invasion of pathogens, improvement of the epithelial barrier function, and so on (Saxelin et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Lactic acid bacteria could exert beneficial effect on humans due to their potential health-promoting properties, such as inhibition on invasion of pathogens, improvement of the epithelial barrier function, and so on (Saxelin et al, 2005). Adhesion and colonization of lactic acid bacteria strains in the gastrointestinal tract is considered one of the main criteria for the selection of potential probiotics, as it may increase their persistence in the intestine and thus allow their exertion of probiotic effects (Kolida et al, 2006;von Ossowski et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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2015

IJAPR

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Bacterial aggregation and hydrophobicity of lactic acid bacteria strains isolated from Chinese traditional fermented food were performed in order to assess a correlation with their adhesion abilities by using intestinal Caco-2 cell line in vitro model. In this study, no correlation existed between hydrophobicity/autoaggregation and adhesion of the strains belonging to different species, whereas a positive correlation (P < 0.01) existed between hydrophobicity/autoaggregation and adhesion of the strains belonging to the same species. When treated with 5 M LiCl, the autoaggregation and adhesion ability of some lactic acid bacteria strains decreased, indicating that surface-bound proteins and other macromolecules were involved in the adhesion and autoaggregation abilities. Hydrophobicity, autoaggregation, and coaggregation abilities of lactic acid bacteria strains could be used as the preliminary criteria for selecting strains with high adhesion ability.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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2015

IJAPR

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“…The use of natural antimicrobials may conduce to control the disordered growth of oral microbiota, thus overwhelming problems caused by species resistant to conventional antimicrobials. 10,11 Natural materials have proved antibacterial action mainly because most plants used in alternative medicine are composed of flavonoids, which act on bacterial cells disrupting the cytoplasmic membrane and inhibiting the enzymatic activity. 12 Turkey is a Mediterranean country rich in medicinal and aromatic plants.…”

Section: Introductionmentioning

confidence: 99%

Investigation of antimicrobial activities of some herbs containing essential oils and their mouthwash formulations

Kulaksiz

Okur

et al. 2018

tjps

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Amaç: Türkiye'de halk arasında yaygın olarak kullanılan bitkilerden elde edilen uçucu yağların antimikrobiyal etkilerini araştırmak ve bu etkilerden yola çıkarak ağız enfeksiyonlarında kullanılmak üzere gargara formülasyonu hazırlamaktır. Gereç ve Yöntemler: Uçucu yağlar, bitkilerden (Origanum vulgare L. spp. hirtum, Rosmarinus officinalis L., Laurus nobilis L., Salvia fruticosa Mill.) su distilasyonu metodu ile Clevenger apareyi kullanılarak elde edilmiştir. Elde edilen uçucu yağlar ve %4,5 ve %9,0 oranlarında uçucu yağların karışımını içeren gargara formülasyonlarının antimikrobiyal aktiviteleri disk difüzyon metodu ve mikrobroth dilüsyon metodu ile araştırılmıştır. Bulgular: Bu çalışmada antimikrobiyal aktivite disk difüzyon metodu ve mikrobroth dilüsyon metoduyla değerlendirilmiştir. İnhibisyon zon çaplarının 7-59 mm arasında değişkenlik gösterdiği belirlenmiştir. Uçucu yağlar, mikroorganizmalar üzerine saf olarak uygulandığında statik ve sidal etkinliğin genellikle %50 ve %50'nin üzerinde olduğu gözlenmiştir. %4,5 oranında uçucu yağ karışımı içeren F2 formülasyonunun Staphylococcus aureus ATCC 25923 üzerindeki minimum bakterisidal etkisinin %6,25 olduğu ve diğer tüm mikroorganizmalar üzerinde minimum inhibitör konsantrasyon ve minimum bakterisidal konsantrasyonunun %3,125 olduğu bulunmuştur. Uçucu yağ örneklerinin mikroorganizmalar üzerine saf olarak Objectives: The aim of this study was to prepare pharmaceutical formulations of mouthwashes and examine the antimicrobial activities of essential oils obtained from plants used traditionally in Turkey for oral infections. Materials and Methods: Essential oils were obtained from herbal drugs with water distillation method using of Clevenger apparatus. Antimicrobial capacities of mouthwash formulations containing mixture of essential oils with proportions of 4.5% and 9.0% were examined by disc diffusion method and microbroth dilution method. Results: In study, antimicrobial activity was evaluated by disc diffusion method and microbroth dilution method. The inhibition zone diameters were determined to vary between 7-59 mm. It has been observed that the static and cidal activity was generally 50% and greater than 50%, when pure essential oil samples were applied on microorganism specimens. Formulation F2 containing mixture of essential oils with proportions of 4.5%, showed 6.25% minimum bactericidal effect on Staphylococcus aureus ATCC 25923 and 3.125% the minimum inhibitor concentration and minimum bactericidal concentration on all other microorganisms. It is found that the antimicrobial effect of pure essential oil samples applied on microorganisms, was lower than mouthwashes formulations; the antimicrobial effect of F2 formulation containing mixture of essential oils with proportions of 4.5% was higher than formulation F1 containing mixture of essential oils with proportions of 9%. Conclusion:The results obtained by these methods allow us to conclude that the essential oils and prepared F1 and F2 mouthwash formulations exert activity against microorganis...

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“…Probiotics have recently emerged as the most powerful food grade microbial agents comprise the ability to express numerous health promoting functions of considerable commercial value and therapeutic potential. These are live microorganisms, when administered in adequate amounts confer several health benefits on the host [1], which includes improving cholesterol assimilation [2], lactose tolerance [3], anti-inflammatory [4], antioxidative [5], anticancer [6], antidiarrheal [7], antiallergic [8], antidiabetic [9], antihypertensive effects [10], controlling obesity [11], and also helps in mineral absorption [12]. Probiotics received generally-regarded-as-safe status, due to its safety profile.…”

Section: Introductionmentioning

confidence: 99%

Screening, Characterization, and in Vitro Evaluation of Probiotic Properties of Lactobacillus Strains

Jain

Mehata

Bharti

2017

Asian J Pharm Clin Res

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Objective: The aim of the present investigation was to isolate and identify Lactobacillus strains from dairy and cattle dung samples. Potent isolates were selected for screening by antimicrobial activity; selected lactobacilli were further tested for probiotic properties and adhesive attributes.Methods: Lactobacilli were isolated aseptically on specific de man, rogosa and sharpe medium from dairy and cattle dung samples. Isolates were identified by Gram-staining, motility, catalase, endospore, and carbohydrate fermentation tests. Further, the isolates were screened for antimicrobial activity by disk diffusion assay, and potent lactobacilli were observed for probiotic properties: Acid and bile salt tolerance, gelatinase activity, and autolytic activity. For analyzing the adhesive attributes, isolates were observed for autoaggregation, coaggregation and microbial adhesion to solvents assay. Results: About 12Lactobacillus strains among 98 isolates exhibited maximum antimicrobial activity were further selected for identifying their probiotic and adhesive attributes. Among 12 selected isolates, cell-free supernatant (CFS) of buffalo milk BM10 and goat milk GM10 showed excellent antimicrobial activity, 20.34±0.02 mm against Staphylococcus aureus and 18.65±0.11 mm against Escherichia coli. Isolates showed survival at pH 2 and 3 and can tolerate 0.2-0.3% bile salt concentrations. The GM5 showed maximum autoaggregation (67.04±0.61%) and minimum coaggregation (11.51±0.50%) showed by GM3. The BM10 exhibited maximum adherent value 64.84±1.41% for n-hexadecane. Conclusion:The two lactobacilli, BM10 and GM10 identified as Lactobacillus fermentum and Lactobacillus pentosus on the basis of phenotypic and sugar utilization tests. The CFS of both lactobacilli can be used as antimicrobial agent. Both isolates showed significant results of probiotic and adhesive attributes, therefore, can be evaluated for clinical and therapeutic applications.

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In vitro probiotic properties of Lactobacillus fermentum SK5 isolated from vagina of a healthy woman

Cited by 102 publications

References 34 publications

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Investigation of antimicrobial activities of some herbs containing essential oils and their mouthwash formulations

Screening, Characterization, and in Vitro Evaluation of Probiotic Properties of Lactobacillus Strains

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