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Abstract

Bacterial aggregation and hydrophobicity of lactic acid bacteria strains isolated from Chinese traditional fermented food were performed in order to assess a correlation with their adhesion abilities by using intestinal Caco-2 cell line in vitro model. In this study, no correlation existed between hydrophobicity/autoaggregation and adhesion of the strains belonging to different species, whereas a positive correlation (P < 0.01) existed between hydrophobicity/autoaggregation and adhesion of the strains belonging to the same species. When treated with 5 M LiCl, the autoaggregation and adhesion ability of some lactic acid bacteria strains decreased, indicating that surface-bound proteins and other macromolecules were involved in the adhesion and autoaggregation abilities. Hydrophobicity, autoaggregation, and coaggregation abilities of lactic acid bacteria strains could be used as the preliminary criteria for selecting strains with high adhesion ability.

Lead biosorption efficiency of Levilactobacillus brevis MZ384011 and Levilactobacillus brevis MW362779: A response surface based approach

“…All the selected isolates presented > 50 % hydrophobicity, while highest hydrophobicity (72 %) was recorded for L. brevis MZ384011. The hydrophobicity (%) was greater than the value of previously reported Pb resistant Lactobacillus species ( Duary et al, 2011 ) while results are consistent with the findings of other authors ( Li et al, 2015 , Pringsulaka et al, 2015 , Dlamini et al, 2019 ).…”

Isolation and characterization of lactic acid bacteria with potential probiotic activity and further investigation of their activity by α-amylase and α-glucosidase inhibitions of fermented batters

“…Colonization due to hydrophobicity, autoaggregation, and coaggregation play a vital role in the probiotic evaluation of LAB. In contrast to coaggregation, where they adhere intercellularly to various strains of bacteria, autoaggregation and hydrophobicity allow the microorganisms to adhere to the intestinal mucosa where they bind to colonies of the same group of microorganisms ( del Re et al, 2000 ; Li et al, 2015 ). Bacterial attachment to the human intestinal layer is a complex event involving a variety of elements, including the charges of both human and bacterial cells, their hydrophobicity, extracellular polysaccharides, and proteins (cell surface).…”

Determination of competition and adhesion abilities of lactic acid bacteria against gut pathogens in a whole-tissue model

“…Survival of passage through the challenging conditions in the digestive tract and adhesion to human cells are important for strains to show their health effects in the human gut. The resistance to such conditions is crucial, as exposure to bile salts causes dissociation of the lipid bilayer of cell membranes and disruption of the integrity of cells [45][46][47][48][49][50][51][52][53][54]. Consumption of approximately 1.0x10 6 to 1.0x10 10 viable cells per day is generally accepted as the minimum limit required in order to gain satisfactory probiotic functions in the intestine [15,[55][56][57].…”

“…Studies also concluded that coaggregation, autoaggregation and adhesion abilities are strain specific. This specificity is determined by surface proteins, glycoproteins, teichoic acids and lipoteichoic acids, as well as S-layer proteins [41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60]. The strains used in our study showed high rates of adhesion to intestinal epithelial cells (Table 4).…”

“…Rajoka et al [44] reported that L. rhamnosus showed the highest coaggregation capability with E. coli (49%), Salmonella (52%) and Staphylococcus (29%). Li et al [45] determined the ability of 18 LAB strains to aggregate and adhere to Caco-2 intestinal cells. In the same study, although E. faecalis and L. fermentum strains showed low rates of auto-and coaggregation with Salmonella spp., both strains showed high rates of adhesion to intestinal cells (104.13 and 84.43%, respectively).…”