In vitro probiotic potential of Enterococcus species isolated from tungrymbai, a fermented soybean product of Meghalaya, India

Malakar, Barasa; Das, Ashis; Deka, Sankar Chandra

doi:10.1556/066.2016.0017

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…For bile salts tolerance, all four isolates retained viability, though with a decreasing tendency during 3 hour of incubation, Similar observations have been made in other studies by [20],who observed that the Enterococcus faecium can survive up till 3.5% of bile salt. It has been argued, that the high tolerance to acid and bile salts by Enterococcus faecium is due to the commensalism of this type of bacteria in the gastrointestinal tract of human and animals [21] .…”

Section: Discussionsupporting

confidence: 90%

Rep PCR Characterization of Lactic Acid Bacteria Isolated From Raw Cows’ Milk in Algeria.

Lomani

Nielsen²,

Amine

et al. 2021

Preprint

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Milk and indigenous fermented, milk based products are a rich source of lactic acid bacteria (LAB) and may serve as a source of potential probiotics. In the present study LAB were isolated from raw cows’ milk sampled at five different Algerian farms. A total of 24 Gram positive, catalase negative isolates where identified to species level using a combination of (GTG) 5-based rep-PCR fingerprinting and 16S rRNA gene sequencing. All isolates were identified as Enterococcus faecium and all isolates had highly similar rep-PCR profiles. Four representative isolates were screened for acid tolerance, bile salts tolerance, antimicrobial susceptibility, antibacterial activity and haemolysis. The four selected strains all exhibited good tolerance to low pH (2, 3, and 4), and to bile salts (concentrations of 0.5%, 1%, and 2%) and were sensitive to chloramphenicol, vancomycin, tetracycline, gentamicine and peniciline G, but were resistant to oxaciline. Cell-free supernatants of the four tested strains all inhibited Staphylococcus aureus, Escherichia coli and Listeria monocytogenes but not Salmonella Typhi. No haemolytic activity was observed.

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Section: Discussionsupporting

confidence: 90%

Rep PCR Characterization of Lactic Acid Bacteria Isolated From Raw Cows’ Milk in Algeria.

Lomani

Nielsen²,

Amine

et al. 2021

Preprint

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“…Our findings are consistent with the nontolerance in L. delbruekii isolated from dairy products (Hassanzadazar et al, ), but others have demonstrated the probiotic properties of L. delbrueckii , isolated from home‐made yogurt (Balamurugan et al, ; Handa & Sharma, ). Among the nonstarters, the Enterococcus species (NPL289 and NPL294) were acid‐tolerant, an outcome that aligns with other findings (Malakar, Das, & Deka, ) (Hanchi, Mottawea, Sebei, & Hammami, ). None of the LAB species or strains isolated from commercial yogurt brands had all the desirable probiotic attributes.…”

Section: Discussionsupporting

confidence: 87%

Microbial safety and probiotic potential of packaged yogurt products in Pakistan

Aziz

Zaidi

Bakht

et al. 2019

Journal of Food Safety

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Yogurt is a health food with notable market production and demand. Because of this, we conducted a study on prominent commercial brands of yogurts in Pakistan for microbial content and the probiotic potential of the contained lactic acid bacteria (LAB), in the context of their label claims. All contained viable LAB, but the numbers (cfu g −1 ) varied considerably. Three of the products made explicit probiotic claims, but LAB from these displayed no probiotic attributes per WHO-FAO guidelines. The yogurt starter and nonstarter Lactobacillus strains had no gelatinase or hemolytic activity and exhibited significant antibacterial activity against some human pathogens. One brand with a probiotic claim contained an L. acidophilus strain that showed cholesterol assimilation activity in vitro. Some potential human pathogens that were hemolytic and resistance to β-lactam antibiotics were also detected in the products. The findings demonstrate a need for better quality control and regulation to ensure safety and efficacy of yogurt products.Values are mean of three replicates. ‡ LAB isolated on MRSc and M17 media. | Non-LAB isolated on nutrient and MacConkey media. § Genus and species-specific primers for Lactobacillus, Bifidobacterium, Lactobacillus delbrueckii, Lactococcus lactis, and Streptococcus thermophilu. ¶ Expiry and manufacturing date and contact information provided except lot/batch number. **a: de Man, Rogosa and Sharpe (MRS) medium supplemented with L-cysteine in anaerobic condition; b: M17 in aerobic conditions. † † Neg for the primers used.

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“…plantarum ] ( Zheng et al, 2020 )} used in this study (equivalent to Lpb. plantarum ATCC 8014) has been characterized for its probiotic properties ( Tambekar and Bhutada 2010 ; Sreevani and Kumari 2013 ; Pop et al, 2016 ; Malakar et al, 2017 ; Khalil et al, 2018 ; Monteiro et al, 2019 ). Lpb.…”

Section: Introductionmentioning

confidence: 99%

Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice

et al. 2021

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Lactiplantibacillus plantarum MTCC 2621 is a well-characterized probiotic strain and is reported to possess many health benefits. However, the wound healing potential of this probiotic is yet to be explored. Here, we have assessed the antibacterial, antioxidant, and wound healing activities of cell-free supernatant of Lactiplantibacillus plantarum MTCC 2621 (Lp2621). Lp2621 exhibited excellent antibacterial activity against the indicator bacteria in the agar well diffusion assay. Lp2621 did not show any hemolytic activity. The safety of Lp2621 gel was established using the skin irritation assay in BALB/c mice, and no dermal reactions were observed. The supernatant showed 60–100% protection of A549 cells against H2O2-induced stress. In the scratch assay, Lp2621 accelerated wound healing after 24 h of treatment. The percent wound healing was significantly higher in cells treated with Lp2621 at 18–24 h posttreatment. In an excision wound healing in mice, topical application of Lp2621 gel showed faster healing than the vehicle- and betadine-treated groups. Similar wound healing activity was observed in wounds infected with Staphylococcus aureus. Histological examination revealed better wound healing in Lp2621-treated mice. Topical treatment of the wounds with Lp2621 gel resulted in the upregulation of pro-inflammatory cytokine IL-6 in the early phase of wound healing and enhanced IL-10 expression in the later phase. These findings unveil a protective role of Lp2621 against bacterial infection, oxidative stress, and wound healing.

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In vitro probiotic potential of Enterococcus species isolated from tungrymbai, a fermented soybean product of Meghalaya, India

Cited by 5 publications

References 16 publications

Rep PCR Characterization of Lactic Acid Bacteria Isolated From Raw Cows’ Milk in Algeria.

Rep PCR Characterization of Lactic Acid Bacteria Isolated From Raw Cows’ Milk in Algeria.

Microbial safety and probiotic potential of packaged yogurt products in Pakistan

Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice

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