Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice

Abstract

Lactiplantibacillus plantarum MTCC 2621 is a well-characterized probiotic strain and is reported to possess many health benefits. However, the wound healing potential of this probiotic is yet to be explored. Here, we have assessed the antibacterial, antioxidant, and wound healing activities of cell-free supernatant of Lactiplantibacillus plantarum MTCC 2621 (Lp2621). Lp2621 exhibited excellent antibacterial activity against the indicator bacteria in the agar well diffusion assay. Lp2621 did not show any hemolytic activity. The safety of Lp2621 gel was established using the skin irritation assay in BALB/c mice, and no dermal reactions were observed. The supernatant showed 60–100% protection of A549 cells against H2O2-induced stress. In the scratch assay, Lp2621 accelerated wound healing after 24 h of treatment. The percent wound healing was significantly higher in cells treated with Lp2621 at 18–24 h posttreatment. In an excision wound healing in mice, topical application of Lp2621 gel showed faster healing than the vehicle- and betadine-treated groups. Similar wound healing activity was observed in wounds infected with Staphylococcus aureus. Histological examination revealed better wound healing in Lp2621-treated mice. Topical treatment of the wounds with Lp2621 gel resulted in the upregulation of pro-inflammatory cytokine IL-6 in the early phase of wound healing and enhanced IL-10 expression in the later phase. These findings unveil a protective role of Lp2621 against bacterial infection, oxidative stress, and wound healing.

Hyaluronic Acid Modified Nanostructured Lipid Carrier for Targeting Delivery of Kaempferol to NSCLC: Preparation, Optimization, Characterization, and Performance Evaluation In Vitro

“…Clone formation assay was performed to explore the inhibitory ability of different preparations in the growth of tumor cells [ 57 ]. A549 cells were inoculated into 6-well plates (500 cells/well), with the treatment of KA, KA-NLC, HA 20 -KA-NLC, and HA 130 -KA-NLC for 14 days.…”

Synergistic Inhibitory Effect of Honey and Lactobacillus plantarum on Pathogenic Bacteria and Their Promotion of Healing in Infected Wounds

“…Moreover, topical administration of L. plantarum accelerated the healing of chronic diabetic foot ulcers and infected burn wounds by altering infection, angiogenesis, macrophage phenotype, and neutrophil response [ 12 , 13 ]. It was also found that L. plantarum supernatant has protective effects against bacterial infection, oxidative stress, and wound healing [ 14 , 15 ]. According to the studies mentioned above, probiotics and their metabolites may be able to treat bacteria that are resistant to antibiotics.…”

The Probiotic Strains Bifidοbacterium lactis, Lactobacillus acidophilus, Lactiplantibacillus plantarum and Saccharomyces boulardii Regulate Wound Healing and Chemokine Responses in Human Intestinal Subepithelial Myofibroblasts

“…Specifically, this bacterial strain and its metabolome mediate the production of both anti-inflammatory IL-10 and pro-inflammatory cytokines, such as IFN-γ, IL-1β, IL-2, IL-6, IL-10, IL-12, IL-17 and TNF-α [ 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. Heat-inactivated or live L. plantarum or its supernatants could also promote skin wound healing, while simultaneously downregulating fibrotic disposition [ 15 , 16 , 17 ].…”