In vitro platelet function of platelet concentrates prepared using three different apheresis devices determined by impedance and optical aggregometry

Abstract

Inlet-line diversion decreased bacterial contamination during two-arm collections by more than 46%. Concurrently, doubling the sample volume was associated with a 54% relative increase in culture sensitivity. These interventions act cooperatively to decrease bacterial risk.

In vitroassessment of platelet concentrates with multiple electrode aggregometry

“…Jilma-Stohlawetz et al used the method to compare different apheresis devices and to study the effect of plasma removal on platelet aggregation [13,14] and Ostrowski et al evaluated the effect of pathogen reduction technologies [15]. The latter study also found deterioration in platelet aggregation over time in platelet concentrates, as measured with MEA, but no previous studies have attempted to examine the relationship between in vitro aggregation and platelet function in an ex vivo transfusion model.…”

Function and activation state of platelets in vitro depend on apheresis modality

“…There exist a huge number of reports regarding the quality of both random‐donor and apheresis PLT concentrates (PCs) [1–15] but only a few published studies refer to products donated by MCC [16–19]. There are a limited number of published studies [10,15,17] that compare the quality of PCs donated by the two different cell separators Amicus ® (Version 2.52; Fenwal, Inc., Lake Zurich, IL) and Trima Accel ® (Version 5.1; CaridianBCT, Zaventem, Belgium). Skripchenko et al.…”

“…report on the increased activation markers of Amicus ® ‐derived PLTs [10], Jilma‐Stohlawetz et al. [15] show a significantly lower aggregation response of Amicus ® ‐derived PLTs. On the other hand, Picker et al.…”

Cold storage of platelets in platelet additive solution: an in vitro comparison of two Food and Drug Administration–approved collection and storage systems

“…Although several PASs exist, only two are currently licensed for use in the United States—Intersol (INT) PAS used with an Amicus collection system (Fenwal) and Isoplate (ISO) PAS used with a Trima collection system (Terumo BCT). The Amicus and Trima apheresis collection systems isolate PLTs by different centrifugation regimens and have been shown to cause differing degrees of PLT activation, which may affect PLT function . It is reported that Amicus‐derived PLTs in 100% plasma display significantly decreased aggregation response to thrombin the day after collection compared to Trima‐derived apheresis PLTs in 100% plasma .…”