Function and activation state of platelets in vitro depend on apheresis modality

Abstract

In multicomponent apheresis, standardized PLT collection is effective and well tolerated. The higher activation of Amicus(®)-derived PLTs may be because of the divergent centrifugation modalities during collection. Possible consequences for the clinical outcome of thrombocytopenic patients will be evaluated in further trials.

Comparative in vitro evaluation of apheresis platelets stored with 100% plasma or 65% platelet additive solution III/35% plasma and including periods without agitation under simulated shipping conditions

“…Percent CD63positive PLTs was somewhat higher with PAS-III compared to plasma components (determined only with Scenarios 3 and 4, data not shown). As found in other studies, [23][24][25] the percent CD63-positive PLTs were markedly less on Day 5 than those of percent CD62P-positive PLTs.…”

Monitoring of Platelet Activation in Platelet Concentrates Using Transmission Electron Microscopy

“…This is not case in PC which are not subjected to pathogen inactivation and in which PLT activation takes place with increasing storage time depending on the suspension medium (plasma or additive solution), leukodepletion, and the quality of storage containers [41,42]. In contrast to cell activation, the number of dead PLT increased with storage time, a result which agrees with recently published papers demonstrating the induction of apoptotic signals [43] and cytokine accumulation [44].…”

High platelet content can increase storage lesion rates following Intercept pathogen inactivation primarily in platelet concentrates prepared by apheresis

“…Finally, it is difficult to speculate on the reasons why apheresis but not buffy coat‐derived concentrates would be more vulnerable in this particular context and for this outcome (low swirling), especially because differences in typical storage lesion markers between buffy coat‐derived and apheresis‐derived platelet concentrates are minor . We do think that the actual platelet content in the final (split) apheresis product is of matter and not necessarily the targeted (high dose) one before splitting because double‐dose or triple‐dose apheresis procedures do not yield products with quickly declining quality, at least when these are split within a couple of hours after donation . In our study, there was no difference between the number of double apheresis donations in the non‐swirling group (2/28) versus the normal swirling group (4/26).…”