In vitro platelet activation, aggregation and platelet–granulocyte complex formation induced by surface modified single-walled carbon nanotubes

Fent, János; Bihari, Péter; Vippola, Minnamari; Sarlin, Essi; Lakatos, Susan

doi:10.1016/j.tiv.2015.04.017

Cited by 7 publications

(5 citation statements)

References 47 publications

(60 reference statements)

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“…CNTs affected blood coagulation by shortening the fibrin clot formation times and inducing hardening (long NH2-MWCNTs) or softening (long COOH-MWCNTs and short NH2-MWCNTs) of the blood clot, respectively [166]. Pristine and functionalized (CONH2-; COOH-; OH-; and PEG-) SWCNTs were shown to activate platelets and to promote platelet-granulocyte complex formation with different intensity in human whole blood [162,167] with exception of OH-SWCNTs. PEG-coated SWCNTs presented the highest platelet activation and aggregation capacity, as well as abundant formation of PLT-granulocyte complex.…”

Section: Carbon Nanotubesmentioning

confidence: 99%

“…Pristine and functionalized (CONH 2 -; COOH-; OH-; and PEG-) SWCNTs were shown to activate platelets and to promote platelet-granulocyte complex formation with different intensity in human whole blood [162,167] with exception of OH-SWCNTs. PEG-coated SWCNTs presented the highest C 2020, 6, 12 of 36 platelet activation and aggregation capacity, as well as abundant formation of PLT-granulocyte complex.…”

Section: Carbon Nanotubesmentioning

confidence: 99%

“…Activation and formation of platelet-granulocyte complexes [162,167]. Aggregation and activation [160] -Activation through classical pathway (C1q binding) [145] No internalization, no toxicity [172].…”

Section: Sw Cnts Pristinementioning

confidence: 99%

“…Shape modification, fusion and hemolysis from bundled SWCNTs [175] Accelerated thrombus formation in the microcirculation [162,163]. Amplification of vascular thrombosis in rats (carotid artery) [160] -COOH Activation and formation of platelet-granulocyte complexes [167] Activation of the contact pathway [159] -Dose-and time-dependent hemolysis [173] Toxic effect on erythrocytes and transient anemia in mice [173] MW CNTs Pristine Aggregation, activation [160,165] and formation of PMPs [164]. Minimal activation [158] Activation of the intrinsic pathway [158] Activation through classical and alternative pathway [145].…”

Section: Sw Cnts Pristinementioning

confidence: 99%

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Hemocompatibility of Carbon Nanostructures

Fedel

2020

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Carbon nanostructures (CNs), such as carbon nanotubes, fullerenes, carbon dots, nanodiamonds as well as graphene and its derivatives present a tremendous potential for various biomedical applications, ranging from sensing to drug delivery and gene therapy, biomedical imaging and tissue engineering. Since most of these applications encompass blood contact or intravenous injection, hemocompatibility is a critical aspect that must be carefully considered to take advantage of CN exceptional characteristics while allowing their safe use. This review discusses the hemocompatibility of different classes of CNs with the purpose of providing biomaterial scientists with a comprehensive vision of the interactions between CNs and blood components. The various complex mechanisms involved in blood compatibility, including coagulation, hemolysis, as well as the activation of complement, platelets, and leukocytes will be considered. Special attention will be paid to the role of CN size, structure, and surface properties in the formation of the protein corona and in the processes that drive blood response. The aim of this review is to emphasize the importance of hemocompatibility for CNs intended for biomedical applications and to provide some valuable insights for the development of new generation particles with improved performance and safety in the physiological environment.

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Section: Carbon Nanotubesmentioning

confidence: 99%

Section: Carbon Nanotubesmentioning

confidence: 99%

Section: Sw Cnts Pristinementioning

confidence: 99%

Section: Sw Cnts Pristinementioning

confidence: 99%

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Hemocompatibility of Carbon Nanostructures

Fedel

2020

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“…Morphology of nanotubes was characterized by electron microscopy, and elementary compositions were analyzed by Energy-Dispersive X-ray Spectroscopy (EDS) as described elsewhere [9]. Stock (2 mg/ml) solutions of all the SWCNTs samples were prepared in distilled water using sonication with 4.2x 10 5 kJ/m 3 specific energy in three consecutive steps for 2 min each.…”

Section: Single-walled Carbon Nanotubesmentioning

confidence: 99%

Simultaneous determination of the ratio of live, dead and adherent bacteria in the presence of various single-walled carbon nanotubes

Fent¹,

Lakatos²

2018

J Nanomed

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Objectives: A decrease in bacterial counts as a consequence of their interactions with various carbon nanotubes are usually interpreted as the destruction of viable bacteria. However, changes in adherent properties can also result in a decrease of the detectable bacterial counts.Methods: This paper provides a simple experimental setup and a mathematical formulation for simultaneous determination of ratios of the live/dead and adherent/non-adherent bacteria based on impedance changes. This method is especially useful in case of colored samples where traditional optical methods fail. The usefulness of our method is demonstrated by analyzing the effect of single-walled carbon nanotubes bearing various functional groups exerted on Staphylococcus aureus bacteria.Results: According to our results, pristine single-walled carbon nanotubes destroy Staphylococcus aureus resulting in a decrease in the bacterial counts and do not change the adherent properties of the bacteria. At the same time, amidated single-walled carbon nanotubes have no killing effect and they inhibit the adherence of bacteria to the wall of the sample container. Presence of carboxylated single-walled carbon nanotube neither influences the viability nor the adherent properties of Staphylococcus aureus. Discussion: Our data reveals that nanotubes do not only have the ability to kill bacteria, reducing their viability, but may also affect other deeper details of bacterial life, for example, may change their adhesion ability.

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