In vitro plant regeneration of fig ( Ficus carica L. cv. gular) using apical buds from mature trees

Kumar, Virendra; Radha, A.; Chitta, Suresh Kumar

doi:10.1007/s002990050471

Cited by 42 publications

(20 citation statements)

References 14 publications

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“…A similar result was found by Demiralay et al [5]. Some researchers reported that root formation is high in media containing 2.5 µM IBA (Hepaksoy and Aksoy [8]), or 2 mg IBA·L -1 (Kumar et al [23] Günver and Ertan [6], and Yancheva et al [25]). As a result, medium 4 gave better results than the others.…”

Section: Discussionsupporting

confidence: 70%

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Meristem culture of two fig cultivars in Turkey

Çömlekçioğlu

Küden

Kacar

et al. 2007

Fruits

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--Introduction. Two Turkish fig cultivars, Alkuden and Bursa Siyahi, were propagated by meristem culture to eliminate the fig mosaic virus. The technique of dsRNA analysis was conducted on the in vitro propagated plants to test for virus-free status. Materials and methods. Four different Murashige and Skoog (MS) media complemented with different concentrations of growth hormones (GA 3 , BA and IBA) were compared to study the cultured meristems' survival rate, shoot formation and rooting. Short-and long-term thermotherapy treatments were also applied. Results. For survival rate, 0.2 mg GA 3 ·L -1 + 0.5 mg BA·L -1 gave the most favorable results; for shoot formation, it was the medium with 0.2 mg GA 3 ·L -1 + 2.0 mg BA·L -1 which was the best, while rooting rate was the highest for meristems cultured on MS medium with only 0.1 mg GA 3 ·L -1 . Conclusion. Meristem culture, together with thermotherapy treatment, is recommended to obtain virus-free fig plant material. Although cultured plants seemed to be very healthy, dsRNA virus tests are recommended for sensitive evaluation of the sanitary status of the plants obtained.

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Section: Discussionsupporting

confidence: 70%

“…Researchers such as Günver and Ertan [6], Demiralay et al [5] and Barbosa et al [22] stated that shoot formation was high in medium containing 1 mg BA·L -1 , while other researchers such as Kumar et al, [23] and Brum et al [24] found the best effect with 2 mg BA·L -1 .…”

Section: Discussionmentioning

confidence: 97%

Meristem culture of two fig cultivars in Turkey

Çömlekçioğlu

Küden

Kacar

et al. 2007

Fruits

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“…Trabalhos in vitro com diversas variedades de Ficus carica têm sido restritos à produção de plantas livres de vírus, mediante cultura meristemas (HAELTERMAN e DOCAMPO, 1994;NOBRE et al, 1998;DEMIRALAY et al, 1998;GUNVER et al, 1998;KUMAR et al, 1998). No Brasil, poucas são as pesquisas realizadas com o cultivo in vitro da figueira 'Roxo de Valinhos', embora os resultados observados por Barbosa et al (1992), Anjos Sobrinho et al (1998 e Brum (2001) confirmem a possibilidade da propagação in vitro da figueira.…”

Section: Introductionunclassified

“…A utilização da cinetina no meio de cultura diminuiu a formação de calos em Ficus carica (JORDAN e ITURRIAGA, 1980). Um procedimento viável para indução múltipla de brotos e regeneração de plântulas foi desenvolvido com gemas apicais coletadas de árvores adultas de Ficus carica 'Gular', utilizando meio MS suplementado com BAP 2 mg.L -1 e ANA 0,2 mg.L -1 (KUMAR et al, 1998). Resultado semelhante foi verificado por Brum (2001) com a cultivar Roxo de Valinhos, que observou menor incremento no número total de brotos, com a associação de concentrações superiores a 2 mg.L -1 de BAP e baixas concentrações de ANA.…”

Section: Introductionunclassified

Multiplicação in vitro DE Ficus carica L.: efeito da cinetina e do ácido giberélico

Fráguas

Pasqual²,

Pereira³

2004

Ciênc. agrotec.

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RESUMOA cultura da figueira é afetada pelo vírus-do-mosaico e a cultura de tecidos é uma alternativa para se proceder à limpeza clonal. Neste trabalho, objetivou-se estudar o efeito da cinetina e GA3 na multiplicação in vitro da figueira. Segmentos nodais foram inoculados em meio de cultura WPM contendo as seguintes combinações de cinetina (0; 0,5; 1; 2 e 4 mg.L -1 ) e GA3 (0, 2, 4, 6 e 8 mg.L -1 ). Avaliaram-se número e comprimento dos brotos, peso da matéria fresca e seca da parte aérea, nú-mero de raízes, peso da matéria fresca e seca do sistema radicular e de calos. A utilização de 0,5 mg.L -1 de cinetina promoveu melhor taxa de multiplicação in vitro de Ficus carica. O GA3 reduziu a formação e multiplicação dos brotos e induziu ao estiolamento, à hiperidricidade, clorose e necrose apical das plântulas.Termos para indexação: Propagação in vitro, figueira, reguladores de crescimento. ABSTRACTThe fig culture is affected by mosaic virus and the tissue culture is an alternative in the clonal cleaning. The kinetin and GA3 effects on in vitro fig multiplication was studied. Nodal segments were inoculated in WPM culture medium containing the following combination of kinetin (0, 0.5, 1, 2 and 4 mg.L -1 ) and GA3 (0, 2, 4, 6 and 8 mg.L -1 ). The number and length, fresh and dry weigh matter of aerial part, number of roots, fresh and dry weight matter of root system and fresh and dry weight matter of callus were evaluated. The use of kinetin 0.5 mg.L -1 promoted higher rates of in vitro Ficus carica multiplication. The GA3 reduced the formation and shoot multiplication, and induced etiolation, hyperhydricity, clorosis and apical necrosis at the plantlets.

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“…This kind of vegetative propagation insures uniformity, relatively low multiplication rates because those materials can be obtained only from upright branches which results in poor rooting (Kumar et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Use of standard and setup of non conventional techniques for the elimination of viruses associated with Fig Mosaic Disease (FMD) in fig germplasm (Ficus carica L.)

Yahyaoui¹

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In vitro plant regeneration of fig ( Ficus carica L. cv. gular) using apical buds from mature trees

Cited by 42 publications

References 14 publications

Meristem culture of two fig cultivars in Turkey

Meristem culture of two fig cultivars in Turkey

Multiplicação in vitro DE Ficus carica L.: efeito da cinetina e do ácido giberélico

Use of standard and setup of non conventional techniques for the elimination of viruses associated with Fig Mosaic Disease (FMD) in fig germplasm (Ficus carica L.)

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