RESUMOObjetivou-se com este trabalho verificar o efeito da matéria orgânica como componente de substratos para mudas micropropagadas de abacaxizeiro cv. Pérola em fase de aclimatização. Mudas micropropagadas de abacaxizeiro cv. Pérola, selecionadas de acordo com o peso (aproximadamente 2 g), foram plantadas em bandejas de isopor de 72 células de 120 cm 3 contendo proporções de substratos com terra, esterco bovino, Plantmax e matéria orgânica. O delineamento estatístico utilizado foi o inteiramente casualizado com 4 repetições e 3 plantas por parcela. As avaliações foram feitas 90 dias após o plantio, quando se avaliou: altura da planta, comprimento de raiz, peso de matéria fresca de raiz e parte aérea, peso de matéria seca de raiz e parte aérea e número de folhas. Com os resultados obtidos, conclui-se que a matéria orgânica tem efeito significativo no desenvolvimento das mudas. Com o substrato contendo terra, esterco e Plantmax foi obtido o melhor desenvolvimento das raízes e parte aérea.Termos para indexação: Cultura de tecidos, Ananas comosus, matéria orgânica. ABSTRACTThe objective of this work was to evaluate the effect of the organic matter as component of substrata for micropropagated plants of pineapple cv. Pérola in the acclimatization phase. Micropropagated plants were selected according to the weight (approximately 2g) and planted in trays with 72 cels of 120 cm 3 , containing substrata made of soil, bovine manure, Plantmax and organic matter at different proportions. At 90 days after planting, the following parameters were evaluated: height of the plant, root length, weight of fresh matter of root and aerial part, dry matter weight of root and aerial part and number of leaves. The obtained results allowed to conclude that the organic matter has significant effect on the development of the seedlings. The best results for the development of the roots and aerial part were obtained with substratum containing soil, manure and Plantmax.
ETIOLATED IN MICROPROPAGATION OF CV. PÉROLA PINEAPPLE PLANT ABSTRACTIt was aimed to produce micropropagated plantlets of pineapple cv. Pérola by using the etiolated technique and subsequent recovery of etiolated shoots. Two experiments were carried out. In the first, the stalks used as explant were obtained from in vitro shoots established without leaves. media (10.26 shoots at 40 days in the darkness). The MS medium without growth regulators, was significantly better for etiolated shoots when the explants were maintained for 80 days in the darkness, presenting 10.86cm mean length. In the second experiment, the etiolated shoots, with or without apex, were put horizontally in plates containing the three culture media described before. The MS medium supplemented with ANA 1.8mg.L -1 and BAP 2mg.L -1 induced the best result for total shoots number (10.61 shoots when the apex were eliminated).
RESUMOCom o objetivo de induzir a multiplicação em explantes de oliveira, segmentos nodais oriundos de plântulas mantidas in vitro foram excisados e inoculados em tubos de ensaio contendo meio de cultura MS suplementado com 2 g L -1 de carvão ativado, BAP (0, 1, 2 e 4 mg L -1 ) e ANA (0; 0,01; 0,1 e 1 mg L -1 ), solidificado com 6 g L -1 de ágar e pH ajustado para 5,8. Durante 100 dias, os explantes foram mantidos em sala de crescimento a 25±1ºC, intensidade luminosa de 32 µmoles.m -2 .s -1 e fotoperíodo de 16 horas. Não houve indução de brotações nos segmentos nodais. O maior comprimento da parte aérea foi obtido com 0,1 mg L -1 de ANA na ausência de BAP. O meio de cultura sem BAP proporcionou maior peso de matéria fresca da parte aérea. Termos para indexação:Cultivo in vitro, proliferação, segmentos nodais, BAP, ANA. ABSTRACTThis work had the objective to induce olive multiplication. Nodal segments from in vitro plantlets were excised and inoculated in test tubes containing MS culture medium supplemented with activated charcoal (2 g L -1 ), BAP (0, 1, 2 and 4 mg L -1 ), NAA (0; 0.01; 0.1 and 1 mg L -1 ), agar (6 g L -1 ) and pH adjusted to 5.8. The explant were maintained in growth room to 25±1°C, 32 µmoles.m -2 .s -1 light intensity and 16 hours photoperiod for 100 days. There was not shoots induction in the nodal segments. Larger length of aerial part were obtained with ANA 0.1 mg L -1 in the BAP absence. Culture medium without BAP provides larger weight of fresh matter of the aerial part.
RESUMOA cultura da figueira é afetada pelo vírus-do-mosaico e a cultura de tecidos é uma alternativa para se proceder à limpeza clonal. Neste trabalho, objetivou-se estudar o efeito da cinetina e GA3 na multiplicação in vitro da figueira. Segmentos nodais foram inoculados em meio de cultura WPM contendo as seguintes combinações de cinetina (0; 0,5; 1; 2 e 4 mg.L -1 ) e GA3 (0, 2, 4, 6 e 8 mg.L -1 ). Avaliaram-se número e comprimento dos brotos, peso da matéria fresca e seca da parte aérea, nú-mero de raízes, peso da matéria fresca e seca do sistema radicular e de calos. A utilização de 0,5 mg.L -1 de cinetina promoveu melhor taxa de multiplicação in vitro de Ficus carica. O GA3 reduziu a formação e multiplicação dos brotos e induziu ao estiolamento, à hiperidricidade, clorose e necrose apical das plântulas.Termos para indexação: Propagação in vitro, figueira, reguladores de crescimento. ABSTRACTThe fig culture is affected by mosaic virus and the tissue culture is an alternative in the clonal cleaning. The kinetin and GA3 effects on in vitro fig multiplication was studied. Nodal segments were inoculated in WPM culture medium containing the following combination of kinetin (0, 0.5, 1, 2 and 4 mg.L -1 ) and GA3 (0, 2, 4, 6 and 8 mg.L -1 ). The number and length, fresh and dry weigh matter of aerial part, number of roots, fresh and dry weight matter of root system and fresh and dry weight matter of callus were evaluated. The use of kinetin 0.5 mg.L -1 promoted higher rates of in vitro Ficus carica multiplication. The GA3 reduced the formation and shoot multiplication, and induced etiolation, hyperhydricity, clorosis and apical necrosis at the plantlets.
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