In vitro photodynamic antimicrobial activity of methylene blue and endoscopic white light against Helicobacter pylori 26695

Choi, Sang-Bang; Lee, Hae Kyung; Chae, Hiun Suk

doi:10.1016/j.jphotobiol.2010.07.004

Cited by 30 publications

(16 citation statements)

References 24 publications

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“…This result indicated that 4i-PACT can break DNA into pieces. This phenomenon is consistent with the previous results ( Menezes et al, 1990 ; Choi et al, 2010 ; Lin et al, 2012 ). Generally, the reason for multidrug resistance in cellular level is due to the drug only blocked or weakened the partial binding site or signal pathway, while the whole cellular bodies were kept, so it is easy to develop multidrug resistance.…”

Section: Resultssupporting

confidence: 94%

Mechanism and In Vivo Evaluation: Photodynamic Antibacterial Chemotherapy of Lysine-Porphyrin Conjugate

Gao

Meng

et al. 2016

Front. Microbiol.

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Lysine-porphyrin conjugate 4i has potent photosensitive antibacterial effect on clinical isolated bacterial strains such as Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa. The mechanism of photodynamic antibacterial chemotherapy of 4i (4i-PACT) in vitro and the treatment effect in vivo was investigated in this paper. Atomic force microscopy (AFM) revealed that 4i-PACT can effectively destroy membrane and wall of bacteria, resulting in leakage of its content. This was confirmed by dual fluorescent staining with acridine orange/ethidium bromide and measuring materials absorption at 260 nm. Agarose gel electrophoresis measurement showed that 4i-PACT can damage genomic DNA. Healing of wound in rat infected by mixed bacteria showed that the efficiency of 4i-PACT is dependent on the dose of light. These results showed that 4i-PACT has promising bactericidal effect both in vitro and in vivo.

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Section: Resultssupporting

confidence: 94%

Mechanism and In Vivo Evaluation: Photodynamic Antibacterial Chemotherapy of Lysine-Porphyrin Conjugate

Gao

Meng

et al. 2016

Front. Microbiol.

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“…There has been some evidence that treatment of bacteria, such as E.coli (39), H. pylori (40) with MB and light leads to the damage of DNA. In this study, MB was also found to cause L. monocytogenes genomic DNA break‐up.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic Inactivation of Methylene Blue and Tungsten‐Halogen Lamp Light against Food Pathogen Listeria monocytogenes

Lin

Shu-shu

et al. 2012

Photochem & Photobiology

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The aim of this study was to verify the bactericidal effect and the damage of photodynamic inactivation (PDI) using methylene blue (MB) and tungsten‐halogen lamp over Listeria monocytogenes via atomic force microscopy, absorption spectrophotometry, agarose gel electrophoresis, real‐time PCR and SDS‐PAGE. The obtained data indicated that the viability of L. monocytogenes was ca 7‐log reduced by illumination with 10 min tungsten‐halogen lamp light under the presence of 0.5 μg mL−1 MB, and this bactericidal activity against L. monocytogenes of PDI increased proportionally to the concentration of MB and the duration of irradiation. Moreover, after irradiation with MB and visible light, the leakage of intracellular contents was estimated by spectrophotometer at OD260 and OD280, which correlated with morphological alterations. Furthermore, genomic DNA cleavage and protein degradation were also detected after PDI treatment. Consequently, breakage of the membrane, damage of the genomic DNA and degradation of bacterial proteins may play an important role in the mechanisms involved in PDI‐MB bactericidal activity on L. monocytogenes.

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“…mirabilis, P . aeruginosa ) when directly irradiated at considerable power densities (180J/cm2) and in combination with photosensitizing compounds (methylene blue) have been confirmed [Choi et al, 2010;Abu Sayem, 2014]. The use of specific frequency peaks in the blue-violet band (400-420nm) without photosensitizing factors shows microbicidal effect at lower values (36J/cm2) on a wide range of bacterial species [Maclean et al, 2009].…”

Section: Introductionmentioning

confidence: 99%

“…Photodynamic inactivation of microorganisms with the use of light energy emitted in the visible spectrum region (VIS) has recently been validated as a powerful means of contrasting the development of multi-drug resistant bacterial species [St Denis et al, 2011]. Evidences of the microbicidal effect of white light on bacterial strains (H.pylori, P.mirabilis, P.aeruginosa) when directly irradiated at considerable power densities (180J/cm2) and in combination with photosensitizing compounds (methylene blue) have been confirmed [Choi et al, 2010;Abu Sayem, 2014]. The use of specific frequency peaks in the blue-violet band (400-420nm) without photosensitizing factors shows microbicidal effect at lower values (36J/cm2) on a wide range of bacterial species [Maclean et al, 2009].…”

Section: Introductionmentioning

confidence: 99%

Rapid inactivation of SARS-CoV-2 with LED irradiation of visible spectrum wavelenghts

Santis¹,

Luca

Faggioni³

et al. 2020

Preprint

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The COVID-19 pandemic has caused a global health crisis. The difficulty to control the viral spread due to the absence of vaccines and prophylactics measures has raised concerns about prevention and control of SARS-CoV-2. Therefore, it becomes more and more crucial the reduction of environmental risk factors through viral inactivation of aerosols and fomites. Photodynamic inactivation of microorganisms by light energy emitted in the visible spectrum region (VIS), not harmful for mammalian cells and safe for humans, has recently been described. A LED-device emitting a special combination of frequencies in the visible light spectrum was tested on SARS-CoV-2 infected cell surnatant dilutions in order to evaluate the antiviral efficacy. This preliminary in vitro study showed for the first time the ability to inactivate SARS-CoV-2 through LED irradiation of visible spectrum wavelengths. If further and more extensive studies will confirm these data, the usage of this LED could potentially have a big impact on the sanitization of virtually all human environments.

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In vitro photodynamic antimicrobial activity of methylene blue and endoscopic white light against Helicobacter pylori 26695

Cited by 30 publications

References 24 publications

Mechanism and In Vivo Evaluation: Photodynamic Antibacterial Chemotherapy of Lysine-Porphyrin Conjugate

Mechanism and In Vivo Evaluation: Photodynamic Antibacterial Chemotherapy of Lysine-Porphyrin Conjugate

Photodynamic Inactivation of Methylene Blue and Tungsten‐Halogen Lamp Light against Food Pathogen Listeria monocytogenes

Rapid inactivation of SARS-CoV-2 with LED irradiation of visible spectrum wavelenghts

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