In vitro photoautotrophic acclimatization, direct transplantation and ex vitro adaptation of rubber tree (Hevea brasiliensis)

Tisarum, Rujira; Samphumphung, Thapanee; Theerawitaya, Cattarin; Prommee, Wittaya; Cha–um, Suriyan

doi:10.1007/s11240-017-1374-5

Cited by 21 publications

(15 citation statements)

References 32 publications

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“…Using bioreactors in Populus hybrids, sucrose provided higher percentages of survival and rooting when compared without the carbon source (Arencibia et al, 2017). In contrast to the aforementioned results, for in vitro cultivation and ex vitro acclimatization of Hevea brasiliensis, better results for root length and number, survival and rooting were obtained without sucrose and flasks that allowed natural ventilation (Tisarum et al, 2018). Sometimes gradual adaptation to the ex vitro condition may be required for plants grown in in vitro mixotrophic systems to undergo autotrophic growth in greenhouse (Perez et al, 2015).…”

Section: Mixotrophic Effect On In Vitro Rootingmentioning

confidence: 73%

“…Oxidation evaluations according to grade scale evidenced lowest averages without sucrose supplementation (1.21) ( Figure 2D) and S/M ( Figure 2C), but no difference was observed otherwise (p > 0,05). In vitro conditions are stressful to plant growth, and high concentrations of exogenous sugar are a major cause of oxidation during in vitro culture (Tisarum et al, 2018).…”

Section: Mixotrophism Effect On In Vitro Elongation and Adventitious mentioning

confidence: 99%

“…Controlling environmental factors such as the use of mixotrophism is considered important for in vitro morphogenetic response (Heringer et al, 2017). Generally, a positive response to micropropagation among the various factors is due to the reduction or exclusion of the sucrose source, as well as the use of porous membranes, in which the relative humidity of the bottles, characterized by the presence of water and nutrients in the plant, is reduced (Bacillus et al, 2005), thus improving its ex vitro acclimatization process (Saldanha et al, 2012;Jiménez et al, 2015;Batista et al, 2017;Tisarum et al, 2018). Silva et al (2017) reported its positive effect in different species, besides the possibility of variations in the concentration of sucrose, which benefits the cultivation and reduces expenses.…”

Section: Introductionmentioning

confidence: 99%

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MIXOTROPHISM EFFECT ON IN VITRO ELONGATION AND ADVENTITIOUS ROOTING OF Eucalyptus dunnii

Souza

Fernandes

Avelar

et al. 2019

CERNE

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Section: Mixotrophic Effect On In Vitro Rootingmentioning

confidence: 73%

Section: Mixotrophism Effect On In Vitro Elongation and Adventitious mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MIXOTROPHISM EFFECT ON IN VITRO ELONGATION AND ADVENTITIOUS ROOTING OF Eucalyptus dunnii

Souza

Fernandes

Avelar

et al. 2019

CERNE

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“…Therefore, supplemental CO 2 and lighting improved photosynthesis by increasing quantum yield, consequently contributing to higher contents of primary and secondary metabolites (Figures 2 and 3). However, Tisarum et al [64] found that the quantum yield in Hevea brasiliensis cv. 'RRIT413' sharply declined by 39.0% under a CO 2 -enriched condition (1500 µmol·mol −1) , compared with that in ambient CO 2 conditions.…”

Section: Chlorophyll Content Quantum Yield and Stomatal Conductancementioning

confidence: 99%

Carbon Dioxide Enrichment Combined with Supplemental Light Improve Growth and Quality of Plug Seedlings of Astragalus membranaceus Bunge and Codonopsis lanceolata Benth. et Hook. f.

Ren

Jeong

2019

Agronomy

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Astragalus membranaceus Bunge and Codonopsis lanceolata Benth. et Hook. f. are two medicinal species used to remedy inflammation, tumor, and obesity in Eastern medicine. Carbon dioxide (CO2) and supplemental lighting are two methods to enhance the growth, yield, and quality of crops. However, few studies have focused on the synergistic effects of CO2 and the supplemental light source on plug seedlings of medicinal species. In this study, uniform seedlings were grown with no supplemental light (the control) or under one of three supplemental light sources [high pressure sodium (HPS), metal halide (MH), or mixed light-emitting diodes (LEDs)] combined with one of three levels of CO2 (350, 700, or 1050 μmol·mol−1). The supplemental light (100 μmol·m−2·s−1 photosynthetic photon flux density) and CO2 were provided simultaneously from 10:00 pm to 2:00 am every day. The results showed that the supplemental lighting (LEDs, MH, and HPS) greatly improved the seedling quality with greater dry weights (of the shoot, root, and leaf), stem diameter, leaf area, and Dickson’s quality index (DQI) than those of the control in both species. An enriched CO2 at 1050 μmol·mol−1 accelerated the growth and development of plug seedlings, evidenced by the increased root and leaf dry weights, stem diameter, and DQI compared to the those from the other two CO2 enrichment levels. Moreover, LEDs combined with 1050 μmol·mol−1 CO2 not only increased the contents of soluble sugars but also the starch content. However, an enriched CO2 at 700 μmol·mol−1 was more suitable for the accumulation of total phenols and flavonoids. Furthermore, LEDs combined with 700 or 1050 μmol·mol−1 CO2 increased the chlorophyll, quantum yield, and stomatal conductance at daytime and nighttime for A. membranaceus and C. lanceolata, respectively. In conclusion, the data suggest that LEDs combined with CO2 at 1050 μmol·mol−1 is recommended for enhancing the growth and development of plug seedlings of A. membranaceus and C. lanceolata.

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“…Micropropagation is a clonal propagation technique that aseptically propagates plants in vitro using culture media as substrate under controlled environmental conditions (Cobo et al, 2018;Nunes et al, 2018;Singh et al, 2019;Tisarum et al, 2018). To approach the challenge of producing plant material for commercial planting of arrow cane (G. sagittatum), the Plant Biotechnology Research Group (GIBV, for its acronym in Spanish) at the Universidad de Córdoba has worked to develop a micropropagation protocol for arrow cane cv.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of arrow cane, Gynerium sagittatum (Aubl.) P. Beauv. cv. Criolla, Criolla 1, and Martinera, in a double-phase medium

Diaz¹,

Padrón²,

Álvarez

2021

CTA

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To evaluate the micropropagation response of arrow cane, Gynerium sagittatum (Aubl.), plants using a double-phase medium in the multiplication stage, explants consisting of stem sections with axillary meristems from cultivars Criolla, Criolla 1, and Martinera were established in vitro in a semisolid medium. Then, they were multiplied using a double-phase medium supplied at several Benzylaminopurine (BAP) concentrations (0.0, 0.5, 1.0, 1.5, and 2.0 mg/L), followed by rooting in a culture medium supplied at several Naphthaleneacetic acid (NAA) concentrations (0.0, 0.5, 1.0, 1.5, and 20 mg/L). Both multiplied unrooted and rooted microshoots were transferred ex vitro. Treatments were distributed with a completely randomized design; data were analyzed with an ANOVA and means separated with Tukey’s test. Explants from Criolla and Martinera cultured with 0.5 mg/L BAP resulted in higher multiplication rates. All microshoots transferred to the rooting medium rooted, although NAA significantly increased the number of roots and reduced root length. Plants from all three cultivars, in vitro rooted or unrooted transferred to ex vitro conditions, showed 100 % survival and adaptation. For Criolla and Martinera, 0.5 mg/L BAP statistically increased shoot multiplication rates and NAA increased adventitious root formation and reduced root length. Plants of all cultivars survived and adapted 100 % to ex vitro conditions.

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In vitro photoautotrophic acclimatization, direct transplantation and ex vitro adaptation of rubber tree (Hevea brasiliensis)

Cited by 21 publications

References 32 publications

MIXOTROPHISM EFFECT ON IN VITRO ELONGATION AND ADVENTITIOUS ROOTING OF Eucalyptus dunnii

MIXOTROPHISM EFFECT ON IN VITRO ELONGATION AND ADVENTITIOUS ROOTING OF Eucalyptus dunnii

Carbon Dioxide Enrichment Combined with Supplemental Light Improve Growth and Quality of Plug Seedlings of Astragalus membranaceus Bunge and Codonopsis lanceolata Benth. et Hook. f.

Micropropagation of arrow cane, Gynerium sagittatum (Aubl.) P. Beauv. cv. Criolla, Criolla 1, and Martinera, in a double-phase medium

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