In vitro ovicidal activity of Brongniartia montalvoana against small ruminant gastrointestinal nematodes

Cortes-Morales, Jorge Alberto; Olmedo-Juárez, Agustín; González-Cortazar, Manasés; Zamilpa, Alejandro; López-Arellano, M A; Blé-González, Ever A.; Tapia‐Maruri, Daniel; Flores‐Franco, Gabriel; Salinas-Sánchez, David Osvaldo

doi:10.1016/j.exppara.2022.108336

Cited by 6 publications

(6 citation statements)

References 49 publications

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“…The interaction of some phenolic compounds with the egg membrane causes structural changes affecting its permeability, oxygen exchange, and release of substances and enzymes involved in eggshell degradation and this allows no release of the larvae ( Rogers & Brooks, 1977 ; Vargas-Magaña et al, 2014 ; Engström et al, 2016 ). Recent studies with confocal laser microscopy scanning analysis have demonstrated important scientific evidence concerning the possible mechanism of action of the flavonol isokaempferide and coumaric acid on the H. contortus egg hatching process, where there is colocalization of the compounds with the egg membrane ( Cortes-Morales et al, 2019 , 2022 ). These findings indicate that these compounds pass through the external cuticle of the eggs and there is a chemical interaction between the compounds with the H. contortus morula or embryo ( Cortes-Morales et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching

Becerril-Gil,

Estrada-Flores,

González-Cortazar

et al. 2024

Rev. Bras. Parasitol. Vet.

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The aim of this study was to assess the in vitro ovicidal activity of an ethyl acetate extract from Arceuthobium vaginatum (EtOAc-E) and their subfractions (AvR5-AvR14) against Haemonchus contortus using the egg hatching inhibition (EHI) test. The EtOAc-E and subfractions were tested at 0.12-2.00 and at 0.015-2.0 mg/mL, respectively. Distilled water and methanol (2%) were used as negative controls and Thiabendazole (0.10 mg/mL) as a positive control. Treatments with a dependent effect on concentration were subjected to regression analysis to determine the effective concentrations (EC50 and EC90). The major secondary compounds present in the extract and subfractions were identified by high performance liquid chromatography (HPLC). The EtOAc-E and AvR9 exhibited the best ovicidal effect recording 97.5 and 100% of EHI at 0.25 mg/mL, respectively. The EtOAc-E and AvR9 displayed an EC50= 0.12 and 0.08 mg/mL, respectively. The HPLC analysis in the EtOAc-E and bioactive fractions indicated the presence of a polyphenol, glycosylated flavanones, quercetin glucoside, cinnamates, coumarin, cinnamic acid derivative, ferulic acid, coumarate, naringenin, protocatechuic acid and naringin. Results demonstrated that A. vaginatum extract and fraction is able to inhibit the egg hatch process of H. contortus and could be a viable option for the control of small ruminant haemonchosis.

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Section: Discussionmentioning

confidence: 99%

Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching

Becerril-Gil,

Estrada-Flores,

González-Cortazar

et al. 2024

Rev. Bras. Parasitol. Vet.

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“…Likewise, there is scientific evidence that the organic fraction displays the better biological activity in other plant families like Fabaceae. In a recent study, an EtOAc fraction from another Fabaceae: Brongniartia montalvoana inhibited the egg hatching of small ruminant GIN by 99.1% using the lowest concentration (0.8 mg/mL) [ 28 ]. The results observed in our study revealed that the organic fraction was more effective against H. contortus eggs (100% ovicidal effect at 0.62 mg/mL).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Anthelmintic Activity of a Hydroalcoholic Extract from Guazuma ulmifolia Leaves against Haemonchus contortus

et al. 2022

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The purpose of the present study was to assess the ovicidal and larvicidal activity of a hydroalcoholic extract (HAE) and their fractions (aqueous, Aq-F and organic, EtOAc-F) from Guazuma ulmifolia leaves using Haemonchus contortus as a biological model. The egg hatching inhibition (EHI) and larval mortality against infective larvae (L3) tests were used to determine the anthelmintic effect of the treatments. The extract and fractions were tested at different concentrations against eggs and L3. Additionally, distilled water and methanol were used as negative controls and ivermectin as a positive control. The extract and fractions were subjected to HPLC analysis to identify the major compounds. The HAE displayed the highest ovicidal activity (100% EHI at 10 mg/mL). Fractionation of the HA extract allowed increasing the nematicidal effect in the EtOAc-F (100% EHI at 0.62 mg/mL and 85.35% mortality at 25 mg/mL). The phytochemical analysis of the extract and fractions revealed the presence of kaempferol, ethyl ferulate, ethyl coumarate, flavonol, luteolin, ferulic acid, luteolin rhamnoside, apigenin rutinoside, coumaric acid derivative, luteolin glucoside, and quercetin glucoside. These results suggest that G. ulmifolia leaves could be potential candidates for the control of H. contortus or other gastrointestinal parasitic nematodes.

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“…The sheep were housed in metabolic cages and after 2 weeks of adaptation, they have been dewormed in the following scheme: 0.2 mg/mL doramectin (Dectomax®), 5 mg/mL levamisole (Ripercol®), and 2.5 mg/mL monepantel (Zolvix®) orally at 48 h intervals between doses. After 10 days were performed fecal egg counts (epg) using a MacMaster camera, according to Ueno and Gonçalves, [55] for determining a total clearance of natural infection. After confirming a total clearance of parasites by epg and three serials negative coproculture, [55] animals were infected with 5,000 H. contortus third‐sage larvae (L3) of the Kokstad isolated.…”

Section: Methodsmentioning

confidence: 99%

“…After 10 days were performed fecal egg counts (epg) using a MacMaster camera, according to Ueno and Gonçalves, [55] for determining a total clearance of natural infection. After confirming a total clearance of parasites by epg and three serials negative coproculture, [55] animals were infected with 5,000 H. contortus third‐sage larvae (L3) of the Kokstad isolated.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, this effect was attributed to the adhesion of these compounds to the shell of the eggs, having been visualized by confocal microscopy with blue, red and orange fluorescence. [54] Coumarin derivates, from hydroalcoholic extract and fractions obtained from Brongniartia montalvoana, observed as blue fluorescence in the interior of treated eggs by CSLM, caused the inhibition of egg hatch, allowing the larvae development in the egg, but caused deformation of the larvae morphology [55] Nevertheless, in the current study it was not observed other types of fluorescence that could be related to the LdEO constituents adhered to the eggshell or at the interior of eggs, as evidenced in the CLSM micrographs.…”

Section: Confocal Laser Scanning Microscopy (Clsm)mentioning

confidence: 99%

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Chemical Composition, Anthelmintic Activity, and Mechanism of Action of Lippia dominguensis Mold. Essential Oil on Haemonchus contortus

Espino Ureña,

Katchborian‐Neto,

Trinidad

et al. 2023

Chemistry & Biodiversity

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This study is dedicated to God, our families, Ing. Rosina Taveras Macarrulla and the Dominican Republic's sheep and goat farmers.Gastrointestinal nematode parasitism is a major burden to small ruminant production globally, compounded by increasing anthelmintic resistance. Previous studies have identified essential oils (EOs) from the Lippia genus with antiprotozoal and anthelmintic effects. Lippia dominguensis Moldenke (Ld), an endemic specie from the Dominican Republic, has similar popular uses, however, is chemically and pharmacologically yet uncharacterized. Here, we investigated the in vitro anthelmintic activity of LdEO and its ultrastructural effects on eggs and adult nematodes of Haemonchus contortus multidrug-resistant isolated. The GC/MS analysis showed linalool (33.85 %), 1,8-cineole (30.88 %), and δ-terpineol (10.61 %) as the main EO constituents.The LdEO showed an IC 50 = 0.523 mg/mL in the egg hatch test, and the motility in the adult worm motility test was 95.8 % at 1 mg/mL. The confocal scanning laser microscopy of eggs indicated permeabilization or disruption of egg cell membranes as the possible mechanism of action of LdEO. The scanning electron microscopy of adult worms showed wrinkling, undulations, and cuticular disruptions. The LdEO displayed significant in vitro anthelmintic activity on eggs and adult worms of H. contortus. Additionally, the LdEO showed low oral toxicity in mice at 2,000 mg/kg. Thus, additional in vivo studies are justified to determine its anthelmintic efficacy in small ruminants.

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In vitro ovicidal activity of Brongniartia montalvoana against small ruminant gastrointestinal nematodes

Cited by 6 publications

References 49 publications

Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching

Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching

In Vitro Anthelmintic Activity of a Hydroalcoholic Extract from Guazuma ulmifolia Leaves against Haemonchus contortus

Chemical Composition, Anthelmintic Activity, and Mechanism of Action of Lippia dominguensis Mold. Essential Oil on Haemonchus contortus

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