In Vitro Anthelmintic Activity of a Hydroalcoholic Extract from Guazuma ulmifolia Leaves against Haemonchus contortus

Reséndiz-González, Guillermo; Higuera-Piedrahita, Rosa Isabel; Bueno, Alejandro Lara; González-Gardúño, Roberto; Cortes-Morales, Jorge Alberto; González-Cortazar, Manasés; Gives, Pedro Mendoza de; Romero-Romero, Sara Guadalupe; Olmedo-Juárez, Agustín

doi:10.3390/pathogens11101160

Cited by 6 publications

(3 citation statements)

References 35 publications

(40 reference statements)

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“…The effective concentrations 50 and 90 were lower for the EtOAc-F than the HAE, with values of 0.30 and 1.22 against 40.66 and 183.46 mg mL -1 , respectively (Figure 1). Worldwide, the nematicidal effect of hydroalcoholic extracts of diverse plant families such as Fabaceae, Asteraceae, Malvaceae, and Anacardiaceae has been proven on gastrointestinal nematodes in small ruminants, including H. contortus (de Campos-Añaña et al, 2022;López-Rodríguez et al, 2022;Reséndiz-González et al, 2022). The HAE showed ovicidal activity (67.7 % EHI at 100 mg mL -1 ) similar to a report by López-Rodríguez et al (2022) on H. contortus eggs using a hydroalcoholic extract of Leucaena leucocephala leaves.…”

Section: Resultssupporting

confidence: 75%

“…When carrying out the HAE bipartition with ethyl acetate, a mid-polarity solvent, the EtOAc fraction displays the best ovicidal effect against GINs, including H. contortus (Castillo-Mitre et al, 2017;Olmedo-Juárez et al, 2022). The ovicidal effect observed in this study with the EtOAc-F (0.31 mg mL -1 ) can be compared with a similar fraction obtained from a hydroalcoholic extract from Guazuma ulmifolia (Malvaceae), where an inhibiting effect of the hatching of H. contortus higher than 70 % was observed (Reséndiz-González et al, 2022). From 100 mg mL -1 , the HAE displayed 78.4 % larval mortality.…”

Section: Resultsmentioning

confidence: 67%

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OVICIDAL AND LARVICIDAL EFFECTS OF A HYDROALCOHOLIC EXTRACT FROM Cyrtocarpa procera LEAVES AGAINST Haemonchus contortus

De Jesús-Martínez,

Rivero-Pérez,

González-Cortazar

et al. 2024

agro

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This study evaluated the ovicidal and larvicidal effects of a hydroalcoholic extract (HAE) and two fractions—one aqueous (Aq-F) and the other organic (EtOAc-F)—from dehydrated Cyrtocarpa procera leaves on Haemonchus contortus. In addition, the primary compounds in the HAE and the fractions were identified. The egg hatching inhibition (% EHI) and L3 larval mortality (% Mortality) tests were performed. The treatments used in the EHI test were HAE (12.5–200 mg mL-1), Aq-F (5 and 10 mg mL-1), and EtOAc-F (0.62–10 mg mL-1); for larval mortality, HAE (50–200 mg mL-1), Aq-F (20–40 mg mL-1), and EtOAc-F (2.5–40 mg mL-1). Thiabendazole (0.1 mg mL-1) and ivermectin (5 mg mL-1) were used as positive controls, while methanol (3 %) and distilled water were negative controls. The results were analyzed using a completely randomized design and an ANOVA. The main compounds in the extract and fractions were identified using high-performance liquid chromatography. The HAE had a 100 % ovicidal effect at the highest concentration tested, while EtOAc-F had a nearly 100 % ovicidal effect at 1.25 mg mL-1, and Aq-F displayed the lowest ovicidal effect. Regarding larval mortality, the HAE exerted a larvicidal effect close to 80 % at 100 mg mL-1, while EtOAc-F displayed a larval mortality of 71.47 % at 20 mg mL-1. The chemical analysis indicated the presence of gallic acid, derivatives of gallic acid (gallates), kaempferol rutinoside, quercetin glycoside, and luteolin glycoside. This study shows evidence of the ovicidal and larvicidal properties of C. procera, which could make it useful as a natural anthelmintic in the control of H. contortus.

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Section: Resultssupporting

confidence: 75%

Section: Resultsmentioning

confidence: 67%

OVICIDAL AND LARVICIDAL EFFECTS OF A HYDROALCOHOLIC EXTRACT FROM Cyrtocarpa procera LEAVES AGAINST Haemonchus contortus

De Jesús-Martínez,

Rivero-Pérez,

González-Cortazar

et al. 2024

agro

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“…The larvicidal activity of the combination extracts was determined using H. contortus infective larvae (L3), which were obtained from a donor animal. The L3 was obtained from faecal cultures by the modified technique of Corticelli and Lai, reported by Reséndiz- González [ 38 ]. Larvae were activated with light and washed with 4% sucrose.…”

Section: Methodsmentioning

confidence: 99%

Synergistic Interaction Effect of Artemisia cina n-hexane Extract and Tagetes lucida Ethyl Acetate Extract on Haemonchus Contortus

Santiago-Figueroa,

González-Cortazar,

Estrada-Flores

et al. 2024

Acta Parasit.

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Purpose We analysed the possible synergistic activity among active extracts from Artemisia cina and Tagetes lucida combinations on Haemonchus contortus, a nematode parasitising sheep. Methods The work was carried out in vitro on eggs and infective larvae (L3) of H. contortus. The results were analysed with SAS 9.1, applying the ANOVA and Tukey test, and the lethal concentration (LC) values LC50 and LC90 were determined with regression analysis, employing Proc Probit of SAS 9.1. Additionally, the lethal concentration (LC) was calculated with LC50 and LC90 to determine the synergistic effect. Results The results demonstrated a high efficacy of the two plants studied on both nematode eggs and L3 larvae as well as of their combinations. The highest egg hatching inhibition was obtained with a 50/50 combination, and the best larvae mortality was obtained with 25% A. cina and 75% T. lucida at 10 mg/mL. Additionally, this combination showed a synergistic effect. Conclusion The two plant species studied here can be applied as natural anthelmintic alternatives due to their high bioactive effect and synergistic response.

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Preliminary screening of plant essential oils from an oceanic climate zone (NW Spain) for the control of equine cyathostomins

Garduño,

Vázquez,

Monteiro

et al. 2024

Discov Appl Sci

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Cyathostomins (Strongylida: Cyathostominae) are gastrointestinal nematodes (GIN) that affect equines in many parts of the world. Due to anthelmintic resistance (AR), it is necessary to search for control alternatives. The objective of the study was to carry out a screening of 26 essential oils (EOs) from plants to determine in vitro their action on cyathostomins. Essential oils were obtained by hydro-distillation from leaves, flowers, fruits, and seeds of the selected species and used against the eggs of cyathostomins by means of the tests of egg hatching and larval motility inhibition. For each EO, different concentrations were prepared (250, 125, 62.5, 31.3, 15.6, 7.8, 3.9, 2.0, 1.0, 0.5 and 0.24 mg/mL). Oxfendazole at 2.5% mixed with closantel 5% was used as positive control, and PBS served as negative control. Cyathostomin eggs were obtained from feces of infected horses and infective larvae were recovered after coproculture. The in vitro test was carried out 36 h after the challenge with the EOs. The highest production of EOs was obtained from leaves of bay, eucalyptus, mallow, and basil, as well as from seeds of black pepper, coriander, cinnamon, anise, and peel of orange and cloves, cumin, and Lawson’s cypress. The oils extracted from laurel leaves, ground cinnamon, anise, cumin, and coriander seeds had a very high effect on cyathostomins eggs up to concentrations lower than 3.9 mg/mL. Therefore, in addition to the uses against other pathogens such as fungi and bacteria, some EOs might attain great importance as an alternative control strategy in the control of nematode eggs.

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In Vitro Anthelmintic Activity of a Hydroalcoholic Extract from Guazuma ulmifolia Leaves against Haemonchus contortus

Cited by 6 publications

References 35 publications

OVICIDAL AND LARVICIDAL EFFECTS OF A HYDROALCOHOLIC EXTRACT FROM Cyrtocarpa procera LEAVES AGAINST Haemonchus contortus

OVICIDAL AND LARVICIDAL EFFECTS OF A HYDROALCOHOLIC EXTRACT FROM Cyrtocarpa procera LEAVES AGAINST Haemonchus contortus

Synergistic Interaction Effect of Artemisia cina n-hexane Extract and Tagetes lucida Ethyl Acetate Extract on Haemonchus Contortus

Preliminary screening of plant essential oils from an oceanic climate zone (NW Spain) for the control of equine cyathostomins

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