In Vitro Osmotic Stress Tolerance in Potato and Identification of Major QTLs

Gorji, Ahmad Mousapour; Mátyás, Kinga; Dublecz, Zsuzsanna; Decsi, Kincső; Cernák, István; Hoffmann, Borbála; Taller, János; Polgár, Zsolt

doi:10.1007/s12230-012-9268-x

Cited by 17 publications

(12 citation statements)

References 51 publications

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“…It is also possible to tag specific genes related to environmental factors that could have useful applications, for example in molecular ecology. This is because IT uses primers based on allele sequences of functionally characterized genes, and thus specific banding patterns corresponding to plant phenotypes can be identified [103,104]. However, development of such markers depends on the availability of genomic databases with several target sequences for IT markers.…”

Section: Gene-targeted and Functional Markers (Gtms And Fms)mentioning

confidence: 99%

“…The utility of primer pairs in SCoTs was advocated by Gorji et al [210]. SCoT markers are usually reproducible, while primer length and annealing temperature are not the sole factors determining reproducibility [104,210]. They are dominant markers, however, while a number of co-dominant markers are also generated during amplification, and thus could be used for genetic diversity analysis.…”

Section: Targeted Fingerprinting Markers (Tfms)mentioning

confidence: 99%

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Advances in plant gene-targeted and functional markers: a review

et al. 2013

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Public genomic databases have provided new directions for molecular marker development and initiated a shift in the types of PCR-based techniques commonly used in plant science. Alongside commonly used arbitrarily amplified DNA markers, other methods have been developed. Targeted fingerprinting marker techniques are based on the well-established practices of arbitrarily amplified DNA methods, but employ novel methodological innovations such as the incorporation of gene or promoter elements in the primers. These markers provide good reproducibility and increased resolution by the concurrent incidence of dominant and co-dominant bands. Despite their promising features, these semi-random markers suffer from possible problems of collision and non-homology analogous to those found with randomly generated fingerprints. Transposable elements, present in abundance in plant genomes, may also be used to generate fingerprints. These markers provide increased genomic coverage by utilizing specific targeted sites and produce bands that mostly seem to be homologous. The biggest drawback with most of these techniques is that prior genomic information about retrotransposons is needed for primer design, prohibiting universal applications. Another class of recently developed methods exploits length polymorphism present in arrays of multi-copy gene families such as cytochrome P450 and β-tubulin genes to provide cross-species amplification and transferability. A specific class of marker makes use of common features of plant resistance genes to generate bands linked to a given phenotype, or to reveal genetic diversity. Conserved DNA-based strategies have limited genome coverage and may fail to reveal genetic diversity, while resistance genes may be under specific evolutionary selection. Markers may also be generated from functional and/or transcribed regions of the genome using different gene-targeting approaches coupled with the use of RNA information. Such techniques have the potential to generate phenotypically linked functional markers, especially when fingerprints are generated from the transcribed or expressed region of the genome. It is to be expected that these recently developed techniques will generate larger datasets, but their shortcomings should also be acknowledged and carefully investigated.

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Section: Gene-targeted and Functional Markers (Gtms And Fms)mentioning

confidence: 99%

Section: Targeted Fingerprinting Markers (Tfms)mentioning

confidence: 99%

Advances in plant gene-targeted and functional markers: a review

et al. 2013

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“…Lower recombination levels between SCoT markers and the gene/trait than random markers such as RAPDs, ISSRs or SSRs, making it possible to be used directly in marker-assisted breeding programs [ 10 ]. SCoT markers have been successfully used to assess genetic diversity and structure, identify cultivars, and for quantitative trait loci (QTL) mapping and DNA fingerprinting in different species, including rice, tritordeums, sugarcane, grape, potato, mango, myrica rubra, peanut, and garbanzo [ 9 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Potential of Start Codon Targeted (SCoT) Markers to Estimate Genetic Diversity and Relationships among Chinese Elymus sibiricus Accessions

et al. 2015

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Elymus sibiricus as an important forage grass and gene pool for improving cereal crops, that is widely distributed in West and North China. Information on its genetic diversity and relationships is limited but necessary for germplasm collection, conservation and future breeding. Start Codon Targeted (SCoT) markers were used for studying the genetic diversity and relationships among 53 E. sibiricus accessions from its primary distribution area in China. A total of 173 bands were generated from 16 SCoT primers, 159 bands of which were polymorphic with the percentage of polymorphic bands (PPB) of 91.91%. Based upon population structure analysis five groups were formed. The cluster analysis separated the accessions into two major clusters and three sub-clusters, similar to results of principal coordinate analysis (PCoA). The molecular variance analysis (AMOVA) showed that genetic variation was greater within geographical regions (50.99%) than between them (49.01%). Furthermore, the study also suggested that collecting and evaluating E. sibiricus germplasm for major geographic regions and special environments broadens the available genetic base and illustrates the range of variation. The results of the present study showed that SCoT markers were efficient in assessing the genetic diversity among E. sibiricus accessions.

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“…The size of bands ranged from 180 bp by the primer (7) to 1250 bp. by the primer (10). All the Twelve SCoT primers produced 133 band in the species of Caranx melampygus.…”

Section: Resultsmentioning

confidence: 99%