In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium

Brondani, Gilvano Ebling; Araújo, Marla Alessandra de; Alcântara, Berenice Kussumoto de; Carvalho, Janice Guedes de; Gonçalves, Antônio Natal; Almeida, Marcílio de

doi:10.4025/actasciagron.v34i4.15143

Cited by 12 publications

(10 citation statements)

References 40 publications

(15 reference statements)

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“…Analyses for protein amino acids and storage protein distribution in maize grains will be the focus of a separate report since a more detailed proteomic analysis using 2D-PAGE is being currently conducted in which distinct maize lines are being used. The increase of total carbohydrate levels observed in this study may represent a side effect caused by the increased boron (B) in the grains because this element is involved in carbohydrate metabolism (Camacho-Cristóbal et al 2008) and is important for reproduction and organogenesis (Huang et al 2008, Brondani et al 2012.…”

Section: Discussionmentioning

confidence: 99%

Soluble amino acid profile, mineral nutrient and carbohydrate content of maize kernels harvested from plants submitted to ascorbic acid seed priming

Alcântara¹,

Rizzi²,

Gaziola³

et al. 2017

An. Acad. Bras. Ciênc.

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Both the scientific community and society have shown interest in improving the content of amino acids, carbohydrates and mineral nutrients in maize because it represents an important staple food in many developing countries. Earlier studies demonstrated that the treatment of seeds using ascorbic acid (AsAseed priming) enhanced soluble carbohydrates, proteins and soluble amino acids for other species. AsA seed priming in maize showed the potential for reducing abiotic stresses. The effects on grain quality have not been previously demonstrated. This study investigated the impacts of AsA seed priming on maize kernel quality of seeds produced by the plants generated from the primed seeds, based on the amino acid profile and carbohydrate and mineral nutrient contents. AsA seed priming improved the maize kernel quality with respect to the ascorbate content, boron allocation, total carbohydrate content and increased soluble amino acid levels, including serine, tyrosine, alanine, valine, glutamate, arginine, proline, aspartate, lysine and isoleucine, whereas soluble methionine was decreased. Therefore, AsA seed priming can represent a potential technique for improving maize grain quality.

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Section: Discussionmentioning

confidence: 99%

Soluble amino acid profile, mineral nutrient and carbohydrate content of maize kernels harvested from plants submitted to ascorbic acid seed priming

Alcântara¹,

Rizzi²,

Gaziola³

et al. 2017

An. Acad. Bras. Ciênc.

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“…La germinación in vitro se utiliza con éxito para muchas especies y ha demostrado ser superior a otras técnicas como la germinación ex vitro en sustratos o con papel filtro (Ghanbari et al 2012) para ello algunos investigadores emplean medios nutritivos en distintas concentraciones y otros a base de solo agar y agua (Gomes y Canhoto 2003, Brondani et al 2012. En algunos casos la germinación in vitro en medios enriquecidos con sales minerales resulta apropiada especialmente en semillas pequeñas como orquídeas (Roy et al 2011).…”

Section: Introductionunclassified

“…La planta posee tanto un sistema de propagación sexual como vegetativo lo que le proporciona una gran flexibilidad a su establecimiento y perpetuación. Las semillas se diseminan en forma endozoica, en ambiente natural germina después de cinco semanas entre los meses de octubre a diciembre y su germinación es baja respecto a otras mirtáceas (Figueroa 2003).La germinación in vitro se utiliza con éxito para muchas especies y ha demostrado ser superior a otras técnicas como la germinación ex vitro en sustratos o con papel filtro (Ghanbari et al 2012) para ello algunos investigadores emplean medios nutritivos en distintas concentraciones y otros a base de solo agar y agua (Gomes y Canhoto 2003, Brondani et al 2012. En algunos casos la germinación in vitro en medios enriquecidos con sales minerales resulta apropiada especialmente en semillas pequeñas como orquídeas (Roy et al 2011).…”

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Efecto de la concentración y de los componentes del medio de cultivo MS sobre la germinación in vitro de Ugni molinae

2014

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SUMMARYThe objective of the study was to evaluate the effect of concentration and components of the Murashige and Skoog (MS) medium on in vitro germination of Ugni molinae. Two tests were performed; in the first one, different concentrations of the MS medium were used (full-strength MS, ½ MS, ¼ MS, ⅛ MS and MS free). In the second experiment, one of the component was excluded (macro salts, micro salts or vitamins). A tetrazolium test was performed to assess viability. After the first two weeks of sowing, all treatments started germination, which was ascending until approximately 20 days, and then a slowdown was observed, which remained stable between days 40 and 60. All treatments containing any concentration of MS presented lower germination than that presented by the MS free medium, as the media containing some of its components, especially macro salts. The tetrazolium test showed 87 % of viability of the seeds: the maximum germination was 42%, which showed a strong latency. Germination of U. molinae is affected by the presence of the MS medium salts. Based on these results the use of only water and agar are recommended as the in vitro germination medium.Key words: Myrtaceae, tissue culture, MS medium, murtilla, viability. RESUMENEl objetivo de este estudio fue evaluar el efecto de la concentración y los componentes del medio Murashige y Skoog (MS) sobre la germinación in vitro de Ugni molinae. Para ello se realizaron dos ensayos, en el primero, se utilizaron distintas concentraciones del medio MS (MS completo, ½ MS, ¼ MS, ⅛ MS y sin MS) y, en el segundo, se excluyó uno de sus componentes (macrosales, microsales o vitaminas). La viabilidad se determinó mediante la prueba de tetrazolio. Después de las dos primeras semanas de siembra, todos los tratamientos iniciaron la germinación, la que fue en aumento hasta aproximadamente los 20 días, luego se observó una desaceleración hasta mantenerse estable entre los 40 a 60 días. Todos los medios de cultivo que contenían alguna concentración de MS obtuvieron una germinación más baja que el medio sin MS, como también los medios que contenían algunos de sus componentes, especialmente macrosales. La prueba de tetrazolio determinó un 87 % de viabilidad en las semillas en tanto que la germinación máxima alcanzada fue de 42 % lo que evidenció una fuerte latencia. La germinación de U. molinae es muy sensible a la presencia de sales del medio MS razón por la cual se aconseja solo el uso de agua y agar como medio de germinación in vitro.Palabras clave: mirtáceas, cultivo de tejidos, medio MS, murtilla, viabilidad. INTRODUCCIÓNLa murtilla (Ugni molinae Turcz.) es un arbusto endé-mico de Chile, perteneciente a la familia de las mirtáceas. La planta posee tanto un sistema de propagación sexual como vegetativo lo que le proporciona una gran flexibilidad a su establecimiento y perpetuación. Las semillas se diseminan en forma endozoica, en ambiente natural germina después de cinco semanas entre los meses de octubre a diciembre y su germinación es baja respecto a otras mirtáceas (Figue...

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“…organogenesis [121]. Standard and double MS concentrations of boron and calcium have both been used for organogenic culture of E. grandis [122], whereas CaCl 2 has been eliminated from N7 medium during callogenesis, but incorporated during shoot regeneration, of E. urophylla [86]. An MS medium with 4.90 mM NH 4 NO 3 , 5.68 mM K 2 SO 4 , 1 µM CuSO 4 ·5H 2 O, and no KNO 3 has been employed for organogenesis and subsequent shoot development of E. grandis × E. urophylla [123].…”

Section: Organogenesismentioning

confidence: 99%

Tissue Culture of Corymbia and Eucalyptus

Trueman

Hung

Wendling

2018

Forests

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Eucalypts are among the world's most widely planted trees, but the productivity of eucalypt plantations is limited by their often-low amenability to true-to-type propagation from cuttings. An alternative approach to cutting propagation is tissue culture, which can be used to micropropagate valuable genotypes rapidly while simultaneously preserving germplasm in vitro. This review describes the use of tissue culture methods such as shoot culture, organogenesis, and somatic embryogenesis for micropropagating eucalypts. This review also discusses the use of cool storage, encapsulation, and cryopreservation methods for preserving eucalypt germplasm and delaying tissue maturation under minimal-growth conditions.

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In vitro organogenesis of Eucalyptus grandis: effects of boron and calcium

Cited by 12 publications

References 40 publications

Soluble amino acid profile, mineral nutrient and carbohydrate content of maize kernels harvested from plants submitted to ascorbic acid seed priming

Soluble amino acid profile, mineral nutrient and carbohydrate content of maize kernels harvested from plants submitted to ascorbic acid seed priming

Efecto de la concentración y de los componentes del medio de cultivo MS sobre la germinación in vitro de Ugni molinae

Tissue Culture of Corymbia and Eucalyptus

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